Refolding of Inclusion Body Proteins from E Coli

[@sabrinarun2515]

Fantastic video about the Refolding of Inclusion Body Proteins from E Coli, which is the main subject of our research. We also want to learn more the detailed information about its processes.

[@stevenrios6236]

Is it necessary to establish efficient refolding protocols?

[@MrCreativebiomart]

The protocols for dilution or chromatography methods are commonly used. The most important is the buffer and other conditions you used in the process.

[@carolinegreen4771]

Do you have general protocols for protein refolding?

[@MrCreativebiomart]

Unfortunately, no. The process is similar for different proteins, however, the condition details various greatly.

[@candyswift9088]

What refolding method do you suggest for cost effective purpose?

[@MrCreativebiomart]

Dilution is the cheaper way, but the effect is not as good as chromatography. And the subsequent concentration is also laborious.

[@andreabrook7581]

However, now the refolding of inclusion bodies has become a bottleneck in biopharmaceutical.

[@adarshguptak]

Auto voice is creepy, as if she's going to refold me.. left after a minute

[@okpolihenry983]

true