Future of CRISPR (base & prime) and epigenome editing (Interview with Prof David R. Liu)
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- Опубликовано: 10 июл 2024
- Here I had the delight in chatting to Prof David R. Liu, Professor at Harvard University, the Broad Institute, and HHMI. In the interview we discuss his interests in genome editing, how to make precise genome editing safe & efficient using the latest CRISPR tech advances in base editing and prime editing and taking it to the clinic (e.g Beam Therapeutics). Then we talk about the next frontier, epigenome editing.
To see David's publications and lab members visit his website (liugroup.us/) or follow him on Twitter ( davidrliu?lang=en )
TIMESTAMPS
Introduction - 00:00:00
Genome editing before CRISPR - 01:05
Using CRISPR-Cas9 - 07:00
Base editing - 0:10:20
Treating genetic diseases - 28:30
Prime editing & precise genome editing - 34:30
Cas9-SHEE (Cas9-fusions) - 54:00
In vivo delivery of CRISPR - 56:00
Epigenome editing & aging - 59:00
Predictions for next 10 years - 1:04:20
Thank you to my patreon supporters & to L-Nutra for the support in starting the interview series!
Icons in intro; "www.freepik.com/free-photos-v..."Background vector created by freepik - www.freepik.com 
Наука
MY FIRST INTERVIEW!!! Hope you enjoyed!
Phenomenal interview. I fully admit some of the science is over my noggin, but this is like interviewing Henry Ford in 1907, just before the first Model T was released
Really enjoyed this interview Eleanor! I’m following your youtube channel now (and also your twitter). Keep up the good work! 🙏🏼
It was really good. You have some interview skills. Nice you don’t interrupt and allow speaker to fully express thoughts. Seems like you are considering your audience and our less then fully developed knowledge of the subject. Thank you.
Nice first interview. I hope to see more interview on genome and epigenetic editing Maybe Dr. Sinclair next ? Dr. Liu is right publish the paper because in academia it always publish or perish more papers means more "paper". Yeah I expect them to reduce off targeting editing over time, this will be huge for correcting a wide range of genetic aliments. Keep up the good work.
Great job !!!
I have been binge-watching most of your videos this weekend, and I am so happy to finally put a face on such an amazing voice! Been a non-scientist (and a non-native English speaker), I really appreciate that you explain complicated subjects in such a didactic way, as well as the fact that you write while you speak. I wonder, as many others, why your channel does not have more subscribers... Anyway, I am happy I am one of them! A final note, I love when you are smiling while talking, it really ads to your unique way of sharing science! All the best from Argentina.
Thank you!! 😊
De-animated, and not deaminated - I love it.
I appreciate and enjoy the regular animated content, but when you can, more interviews!
Thanks, i hope to!
Omg so this is the man!! He’s much younger than I expected. I wouldn’t be surprised if he goes down in history as the scientist whose work revolutionises modern medicine !
As a science student myself I’m so happy I found your Channel!!
Incredible first interview on your awesome channel! Thanks so much for doing the interview with me!
Wow, great interview! And you are a real person! And congrats on getting David Liu, he´s an awesome scientist.
lol, yes i am real and thank you, totally agree! :)
very nice interview, thank you both for your great works, hope to see more interviews like this one.
Great interview. Great job Eleanor! Thanks to Dr. Liu as well. I appreciate you taking your time to do this for the public! I was especially interested at the end when you started talking about epigenome editing. That is a really cool idea that I hadn’t heard of before. Thanks!
Congratulations of your first interview! This was fantastic. thank you so much for getting good science out there
Amazing interview Sheekey. Well done getting the most out of the time with astounding questions 😊
I’m just starting to learn about DNA editing and anti-aging, and I thoroughly enjoyed this interview. Thank you! Most of the science and terminology went over my head, but I can tell this is a fundamental and powerful field of study! And I could tell that this interview was between two honest and open people, which is powerful. Thank you!
This Interview is the Best Update in genome editing! I’m not specialist in this field but I like science and find all your videos are excellent, mostly because you don’t underestimate your publicum . 👏 thank you
Great interview! Thanks you so much for doing this.
I really enjoyed your interview with Prof David., super digestible and insightful. This is my new favorite channel:)
Oi you are doing great. This is one the most comprehensive channels on aging. Thanks from Dunder.
I am watching your channel very often. So happy to see someone creating videos about aging biology!
It must be a bit overwhelming to be a PhD student and a science RUclipsr at the same time!
Respect from me :)
LOVED THIS INTERVIEW - FINALLY A HIGH LEVEL BIO CHEM INTERVIEW THAT DOESNT SUGAR COAT EVERYTHING!! THANK YOU - SUBSCRIBED NOW!
Found it very interesting. Thank you for making and sharing science all around.
Excellent. Agree 100% with Prof Liu's closing comments.
Thank you! 😊
You guys are awesome and much appreciated. Richar
Excellent interview. Thanks for sharing!
Wow, what a treat! Digging in currently! Thank you!
Love the fact you added time stamps.
Fun and packed with info!
Amazing Interview. Super exciting
Thanks for this interview
Why would three people dislike this video? Such a fascinating topic and well discussed. Thanks
Wow I love your work. Im trying to study aging research and your videos are very helpful. Keep it up!
Keep up the good work! All the best!
I love your work, Sheekey.
Great interview! So much thanks for this!! On a side note, for your later interviews, you might want to make the angle of the cam even with the guest's.
Ms. Sheekey, I am so impressed with you, your intellect and very advanced interviewing skills. So many veteran vloggers have the annoying habit of responding to the interviewee with some sort of sound, like an « um, hummm, yes or the like. » You just nod your head silently. Very professional. And Ms Sheeky, forgive me if I point out that you are far more attractive as a human being than your animation would have ever suggested.
Great Show. I was referred here by Tommy who runs the "Crisper Talk" channel here on RUclips. Lots of good info there too.
But no interviews with genomic stars!!!
Very very good! Above excellent! You are great!
Great interview, crispr technology is the future of health care!
The future is what we join to make the future
Bravo !!! I’m hope to have Epigenetic modulators in five years !!!!
Great job!!!
Absolutely amazing channel!!! Thank you for sharing your vast knowledge. You have made connections for me that have my head spinning!! This interview in particular has monumental gems and confirmed developments that are taking epic historic discoveries like Crispr to its inevitable level of “Greatest discovery in human history” people have no idea what’s coming!! #chromamedicine 👏🏼👏🏼👏🏼🏆🏆🏆🙏🏼🙏🏼🙏🏼
Great interview! Thanks from new subscriber🙂
Loved this interview, and Prof Liu is an amazing researcher.
I was most interested in epigenetics and aging, but I sort of got the impression that he wasn't aware of in vivo reprogramming? He seemed to dodge your question on aging (though this was not the focus of what was a comprehensive interview of his field).
Good job sheekey
Just a suggestion for the future. Could you make a 10 min explanation video to be published prior to the interview of the key things being discussed? It's not easy to understand what's up if you're not in the field.
Great Sheekey!
I have high hopes for Crispr, helping individuals with their conditions, around the world and if it does what it’s meant to do by curing so many people, that have genetic diseases then this could be very positive and a massive breakthrough but like all medicines there’s always risks involved, but I can’t wait to see it curing many and doing it’s work! :)
Peter refer your channel to us. Both of your animated videos and interview are great, learned a lot, thank you!
Thank you!!
Oh yes, your first interview you just did
That's why I saw that I was laughing a lot
Subscribed!
HOW?!?! Not bad for a first guest...
Can you do a video on using stem cells or Exosomes as a delivery vehicle? I know stem cells are promising for crispr. But Im curious if it can be applied to prime or base editing.
Can you tell me how to reset your epigenome. David Sinclair seems to says that he has done so. That I believe should be the priority discussion on reversing aging
Yep. Publish!
I'm planning my future on CRISPR gene editing. I did okay with RNAi, but got out way too early. I'm in this for the next 20+ years
What companies are you invested in
As an investor I always try to learn more about what I invest in. Your videos break complex science down into manageable pieces, so thanks.
One thing...When doing your next interview, can you limit the amount of back and forth between zooms and animations, its a little distracting.
Other than that, great video.
Hi Sheeky love your videos. It seems like you have not set up your computer so that it picks up your mic. The sound is the same according to me! If it is that mic, I suggest a Blue Snowball from Blue microphones. It's probably $50 and totally worth it.
Great job Sheekey. Giving us geezers some hope.
If you do another interview, I'd love to see one with George Church. He's said a few times that there are certain genetic tweaks that are known to reverse aging. But after a year of studying his youtube appearances I am still not really sure what he means. He IS doing a lot of interesting things though: better delivery vectors, editing pigs to grow human organs (successfully apparently), as well as an idea for solving the planetary climate emergency by editing cyanobacteria to be cyanophage resistant & thereby creating a massive carbon sink in the ocean. Go George. Go Sheekey.
Here is a recent aging interview he did with students: ruclips.net/video/hpYOQVpvRW4/видео.html
When will be released the part 2?
😁
I don't mean to say something inappriorpate, but for all you knowledgable people. Do you think CRSP Thereaputics, Beam, or EDITAS is the best investment for a long term investment? (high risk I know)
Sheekey :)
Question: I'm a male and I suffer from a heritable form of Diabetes (MODY). This means that if I make children, 50% of them will inherit my Diabetes. The affected gene has 2 mutations close together. Question: is it possible right now to treat my male procreation cells to eliminate this genetic disease in my male cells, given that these cells are simpler than normal cells and are easy to put in a test tube for treatment? EDIT: I had to rewrite this post because of youtube censor.
Technically it is possible, but from current ethical perspective it's not allowed to edit cells that would be passed to the next generation. See story of Lulu and Nana.
@@ScientistInvests Good point and thank you for the reference. I wonder, however, as half my male reproductive cells have the correct gene, if it would be possible to separate the male reproductive cells with the correct genes from the cells with the incorrect genes. Technically that would not be gene-editing.
One more interviewee vote: Ana Maria Cuervo due to her awesome work in the field of autophagy. Doesn't it seem believable that if we can rescue autophagy, we can continue to ward off many of the diseases of aging?
can i get two votes? One time I voted twice in Wyoming, they were not happy, as it was two votes for Kerry.
Hey, I believe you left a comment on my latest video (I cannot reply to it as RUclips removes before I can do anything as I think you had a weblink).. Anyway, think it was on CMA and reminded me of this comment. Have yet to read the papers though am keen to for the senescence link!
@@TheSheekeyScienceShow huh, yes i've been an avid watcher for a little while now. I didn't realize we can't leave links. I was wondering why my posts were getting removed. weak sauce google. yes I provided some links to Ana Cuervo's lab and the recent paper whereby she shows proof of CMA's ability to tackle Alzheimer's.
Thank you so much for the work you do.
@@TheSheekeyScienceShow Yes it would be interesting to learn more about how CMA & senescence interplay.
Today was the day I learned that metformin & rapamyacin are senamorphics first from you & then later on the Stem-talk podcast with Dr. Kirkland. He also said that senescent cells start the chain that boosts CD38 and hence lowers NAD+.
I pray that we are able to cure so many disease hopefully soon
Hello fellow Sheekey!
Helllloooo!!
My take from this video is that beam and prime will eventually overtake intellia and crispr since they have a better approach
Dr. Sheeky, do you think it is wise to live life now as if I am never going to die. If we are getting there by 2035, someone in their twenties shouldnt even be concerned about aging.
It'd be nice to shut off that gene that moderates pain like they just did in mice.
In some circles of the longevity movement, "deanimate" has a very different meaning. I'd hate to hear that you've "deanimated" any time soon! Hopefully, you'll be active and "animated" for a long time to come, yet!
wow damn interesting, and you are in fact really beautiful
Crawler.
What!? I thought you were a cartoon!
😁
please, copy my comment then Erase it!
what an awesome progression. There you have, dr Eleanor in the flesh"
If you keep it up, you can be sure you will be as known as many other channels as "FoundMyFitness" and others.
* An interview with a Professor at Harvard University, congratulations.
*Oh come on, you are basically an PhD, you can purchase better equipment, now you see your channel is getting many views, especially when more guests start to come, as David Sinclair (this guy just maybe work together with him sometimes) (don't worry, all his interviews are exactly the same and he never tells something different, always the same texts, but he calls lots of attention), or Shin-Ishiro Amai, this one is very rare to give interviews and also a Key person in the area. Believe it or not, Walter O'Brien likes to give interviews too, he is kind of narcissistic, and also likes to talk about the future and himself a lot.
You can buy a new headPhone, a new Cam, Light, and just do a better camera angle.
* All, you will need to lose some time thinking how to make good thumbnails, the one on this video is good, but Close-up Photos of both the interviewers with the title in the middle call more attention! I know you don't have much time, but if your channel grows, is a must.
-*- Checkout *Dr Brad Stanfield* Channel. His main goal is to translate for common mortals like me, complex studies evolving longevity!! (just as you do sometimes)
Also just like you do, he started very small and unpretentiously, and little by little his channel is growing a lot too. Instead of animations, was all him on the couch (check the first videos) with a really bad cam. After changing the scenario his channel tripled the size.
(I detach his channel on mine, because he helps me a lot on the longevity field, and now he has Patrons!). I saw his channel grow from nothing, is so cool.
* You can do a video about the interviews you do, breaking up the ideas discussed, so dumb people like me can understand. Big videos like this are very difficult to normal people to see, but short videos commenting on your own video, not only sum more views, as give more details missed, or that you don't have the time or idea to tell on the interview.
* I know you don't have the time, editing sucks, but is something good if you can find a partner.
* If you earn in dollar or euro, is very sad, but you can pay people in 3º world countries for very cheap, for art or services.
* You can ask for your viewer's help, if you need to reach someone you want to interview!.
*
I make videos on Gene-editing too. Covering latest news. Let me know if it helps you!
Playing God. Rev 9:6 is the end game of CRISPR-CAS9
I hear lots off stuff positive years later nothing. Hoopla. Wishful thinking. 🤷🏻
People still suffering. Everything remains the same sorry.
Satan’s 180 to God’s plan of “everlasting life.”
Revelation 9:6And in those days people will seek death and will not find it. They will long to die, but death will flee from them.
Very clever,not very wise.