Molecular Biology Techniques

RNA/DNA Extraction - @1:20
PCR - @5:20
RACE - @11:40
qRT PCR - @14:40
Western/southern Blot - @25:40
Immunofluorescence Assay - @42:00
Microscopy - @53:30
Fluorescence In Situ - @1:05:00
ELISA - 1:17:20
Coimmunoprecipitation - @1:19:20
Affinity Chromatography - @1:27:00
Mass Spectrometry - @1:30:25
Microdialysis - @1:35:00
Flow Cytometry - @1:36:55
Plasmid Cloning - @1:40:36
Site Directed Mutagenesis - @1:56:10
Transfection/Transduction - @1:59:30
Monosynaptic Rabies Tracing - @2:12:25
RNA Interference - @2:19:27
Gene Knockin - @2:25:30
Cre/Lox + Inducible - @2:31:35
TALENs/CRISPR - @2:42:10
Bisulfite Treatment - @2:55:15
ChIP Seq - @2:58:25
PAR-CLIP - @3:01:58
Chromosome Conformation Capture - @3:05:05
Gel Mobility Shift - @3:07:37
Microarray - @3:09:20
RNA Seq - @3:21:10

[@habilidadesmedicas2]

You are THE best person on RUclips! I can't thank you enough! THANK YOU, THANK YOU!!!

[@ShakespeareCafe]

High quality and comprehensive coverage of the essential techniques
3 1/2 hours!!!
Thank you for the time index, very helpful

[@swsKnife]

Indeed, awesome work. Very benevolent of you @Aj Keefe

[@mumisdead]

This video is unreal, the ultimate resource for molecular biology techniques. Thank you AJ for putting this together!

[@HominidPetro]

Your videos are excellent and your communication is super clear and concise! Thanks so much!

[@kaym4389]

thank you so much for putting together such an insightful video.

[@nabarcheto7950]

Thank you so much for all your effort! Awesome video!

[@crostadiformaggio1]

Thank you so much, this was so useful to review all these techniques at once!

[@meghakalra4099]

very clearly and informatively presented. Thanks a lot! Would highly recommend it!!

[@HelloGTP]

Impressed! Thank you for this vid

[@ilovejuicyxx]

You are awesome! Thanks for this great video.

[@JRockTT]

Wonderful video! Thank you for creating and indexing it! I just want to comment on a couple of items: For plasmids, what you called transfection, we actually call DNA transformation; and also fusion tags are most often placed on the N-term instead of the C-term. But again, it was a wonderful video, and I thank you sincerely!

[@leax4147]

This was so helpful, thank you so much!

[@khadija500]

Thank you so much!

[@Hotchanna]

This is so helpful! Thank you so much!

[@chalknduster2893]

Crisp and clear...👍

[@fatalfakehumanable]

Thank you so much it's informative!

[@jennym5689]

Thank you for a wonderful video! Very useful as I study for my cert

[@Priya-uo3et]

Thank you so much!!

[@kaykarmacrystal]

I think this video alone gets you the ticket to heaven in the afterlife!

[@chunshenghu4207]

ahaha, nice comment.

[@jl2584]

wow ! im amazed ! i should have found ur channel earlier ! excellent thanks so much !

[@hailushibru9962]

Thank you very much

[@praveenrathore315]

Very nice thank you

[@albertescamilla]

thank you sir

[@srinivasprabhu8180]

Respect!

[@fatanehtavasolian1123]

Thanks very much for this video.I want to know the references for these information.

[@shrabonishee5942]

Thank you for the best content 🙏

[@dabinjeong9560]

Gorgeous

[@arielarciniega7442]

god rna dna pcr or rice relevant data you bad man this improve my efort towards my next career i will thank endlessly

[@Viktoria-cb6gv]

Thank you! very helpful :-)

[@muskduh]

thtanks

[@mattbeltrami1590]

Amazing lol

[@kao8598]

Is it possible to radiolabel nestin or doublecortin with technetium or any radioactive material to be detected by gamma camera or pet like if we could cross the bbb to detect neurogenesis

[@user-oq9sm7xp3j]

Hello AJ, is it possible for you to upload the slides in PDFs so that all the students can write down notes on them? Thank you so much in advance!

[@muhammadkashif717]

refrence book please

[@salmaabouelhassan6471]

I have a question regarding the Gene Knockdown through RNAi. So what's the difference between dsRNA, ssRNa, shRNA, and siRNA? Can we use a the antisense only from the siRNA? and if so how can we modify it chemically? And, thanks for the video. It was extremely HELPFUL.

[@marwanmohamed6575]

in the expression vector plasmids 1:56:09 how is the expressed protein going to contain the c terminal protein tag ,,, isnt the cdna we incorporated containd a stop codon and translation will then cease thier?@AJ Keefe

[@marwanmohamed6575]

and if its in the n terminal how we gonna know if the 5* utr wont interrupt our orf or cause a frame shift

[@AJKeefe]

Nope, the expressed protein and GFP are translated together, so the transcript is said to be polycistronic. Our expressed protein will actually have a little GFP protein hanging off the C terminus, which does not typically disrupt it's function (although it could).

[@AJKeefe]

s3.amazonaws.com/bitesizebio/wp-content/uploads/2015/04/fig3.png This image shows what the protein might look like.

[@marwanmohamed6575]

@@AJKeefe first thanks for replying i understand that its a polycistroinc transcript but sholudnt poly cistronic transcirpts producce indepenedent protiens,, i just dont get how they gonna be connected together ,, in the picture u send thier is a linker amino acid seq . from where it come from?,,,

[@hithasunil]

hello sir
what does "biomolecular tools" mean?

[@user-ki2dx3yo6m]

11:40

[@tonytony-lr7ov]

1:17:30

[@tesafilm8447]

just for me 1:50

[@earthworm-filledstomachbyc4254]

Seek Christ Jesus YHVH,