Hi Jenna. Thanks for your question. When setting up PCR reactions, it is common to keep everything on ice. Enzymes are more stable on ice and if you are working with an enzyme that does not have hot-start capabilities meaning that it can be activated at room temperature, you’d like to control for this by keeping it on ice prior to the start of your PCR reaction. For additional technical support inquiries, please contact us at thermofisher.com/askaquestion.
HAha! I needed this! It was only today that my supervisor had a talk with me that some of my PCRs aren't as accurate as they can be...
I prepare in cabinet.and room separate. And clean everytime
Does anyone know why, at the 1:45 minute mark, the test tubes are on ice? I'd love to know, since I'm watching this for research :)
Hi Jenna. Thanks for your question.
When setting up PCR reactions, it is common to keep everything on ice. Enzymes are more stable on ice and if you are working with an enzyme that does not have hot-start capabilities meaning that it can be activated at room temperature, you’d like to control for this by keeping it on ice prior to the start of your PCR reaction.
For additional technical support inquiries, please contact us at thermofisher.com/askaquestion.
we should also wear head cap/nurse cap and a face mask it leads to cross contamination
Face mask is very important to avoid contamination
We agree.