Thanks！
Is it possible to simply get the GOI from an online database and add mutagenic primers, that code for the desired restriction enzymes, to the extended endings? Technically it would make it easier but is it practical in a lab?
Thanks！
Is it possible to simply get the GOI from an online database and add mutagenic primers, that code for the desired restriction enzymes, to the extended endings? Technically it would make it easier but is it practical in a lab?