Preparing a Buffy Coat from Whole Blood

Generating a buffy coat from whole blood samples has several benefits, including concentrating large sample volumes and reducing downstream cell separation handling. This video demonstrates the washed-buffy coat procedure for generating a buffy coat from a whole blood sample while minimizing donor variability. The buffy coat can be used for downstream analyses or isolation of specific cell populations using immunomagnetic cell isolation technologies such as EasySep™.
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[@ishasharma7196]

I only want clean buffy coat what should i do

[@STEMCELLTechnologies]

If you are hoping to get the buffy coat layer without residual red blood cells, you can use the EasySep™ RBC Depletion Reagent (Catalog #18170) to deplete RBCs from buffy coats (cdn.stemcell.com/media/files/pis/10000005628-PIS_00.pdf). Feel free to email us at [e-mail] if you have any more questions.

[@jeffreydani8616]

Im not a doctor or have any medical background but my question is why you extract the buffy coat with some red blood cells? It should be only the buffy coat.

[@STEMCELLTechnologies]

A buffy coat contains leukocytes in a concentrated suspension, originating from whole blood or bone marrow. After centrifugation, we remove the concentrated leukocyte band (this is the buffy coat), plus a small portion of the plasma and concentrated red blood cells (RBCs). The target is to concentrate the leukocytes approximately 5-fold while maintaining an equivalent ratio of leukocytes to RBCs (e.g. collect 2 mL of buffy coat when starting with 10 mL of whole blood). You can also refer to our technical tip “How to Prepare a Buffy Coat from Whole Blood” (www.stemcell.com/how-to-prepare-a-buffy-coat.html) for more information.
If you have any more questions, feel free to email us at [e-mail].