At min 17:30: On Windows 10, after I navigated to the folder, I had to enter: ExtAncMEGA.exe ebgCanc.txt Otherwise, I was getting an error log without the ".exe". PS: thanks for the video :)
Can we still use MEGAX for generation because recently when I tried using MEGAX there was a popup which said not to use the trees generated for the publication. In MEGA7 how do we do for the G+I parameters?
Hello, unfortunately after I work my way through the pipeline I seem to get no ancestral protein. I always get the same "Ancestral Protein Sequence: The DNA sequence is not a coding sequence ( the length is not a multiple of 3). No ancestral protein is calculated." Do you have any advice as to why this is the case? Thank you!
Maybe a little late, but if you are using protein reconstruction, you need to trim your DNA sequence to have a number of bases divisible by 3. Three nucleotide bases make an amino acid.
This video, and the extra script to organise the ancestral output, were fantastic. Thanks so much for making this!
Thanks a lot for this great explanation of ancestral sequences...!
At min 17:30: On Windows 10, after I navigated to the folder, I had to enter: ExtAncMEGA.exe ebgCanc.txt
Otherwise, I was getting an error log without the ".exe".
PS: thanks for the video :)
How do you run the program using amino acid sequences?
Hello, maybe a little late but I would like to know if the ExtAncSeqMEGA is still available. I cannot find it on the given website. :(
Can we still use MEGAX for generation because recently when I tried using MEGAX there was a popup which said not to use the trees generated for the publication. In MEGA7 how do we do for the G+I parameters?
Great video, Can we use the same protocol for ITS sequences and 16S sequences from the company that has sent us?
Hello, unfortunately after I work my way through the pipeline I seem to get no ancestral protein. I always get the same "Ancestral Protein Sequence:
The DNA sequence is not a coding sequence ( the length is not a multiple of 3).
No ancestral protein is calculated." Do you have any advice as to why this is the case?
Thank you!
Maybe a little late, but if you are using protein reconstruction, you need to trim your DNA sequence to have a number of bases divisible by 3. Three nucleotide bases make an amino acid.