Fluorescence In Situ Hybridization (FISH)

( www.abnova.com ) - FISH is a technique used to identify and localize the presence or absence of specific DNA sequences on cells and tissues. You can use FISH probes for the detection of gene amplification, loss and translocation. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originated from a bacterial artificial chromosome (BAC) library. More videos at Abnova www.abnova.com

[@MrTockabocka]

DAPI will generally stain all DNA, while the FISH-Probe will only stain the specified sequence, or the organism (gene) of interest. You could also wash if and stain with a secondary FISH to identify another organism using a different stain and so on. However you will have to have a second form of staining to make it possible to see things using a fluorescence signal

[@autoinjected8484]

loved it very informative thanks a lot

[@yuvaasampath3491]

DAPI used for nuclic acid staining , i love this video.,its use full for me, "merci"

[@salmaelmoudden5275]

Thank you

[@EMMANUELKOLAWOLE-p2s]

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[@Nonoxz]

It is used for contrast. regards

[@topgurl9313]

Thank you very much

[@lotharmayring6063]

verry unsharp and blurry image with fluo

[@TheScharda]

what's the function of ssc ?

[@atheeralaa1056]

Why 500 protease in 50ml

[@truthbetold9233]

1:100 dilution? Which is usually the dilution used for proteases?

[@drmohit21583]

SSC?

[@porcorosso23]

(NaCL + trisodium citrate ) buffer

[@dexterpetallar1843]

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[@Quolon]

Understandable.

[@marcodeluca4068]

something fishy and FISH.