Calculate Mean & Standard Deviation + Create Eye-Catching Bar Graphs for Biological Data

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  • Опубликовано: 18 сен 2024
  • The mean, also known as the average, is a measure of central tendency that identifies the central point of a data set. To calculate the mean, you add up all the values in the data set and then divide by the number of values. For example, if your data set is 3, 5, 7, 9, 11, the mean would be the total of these numbers divided by 5, which equals 7.
    Standard Deviation
    Standard deviation measures how much the values in a data set vary or spread out from the mean. To calculate the standard deviation, you first find the mean, then calculate the difference between each value and the mean, square these differences, average them, and finally take the square root of that average. Using the same data set (3, 5, 7, 9, 11), the standard deviation tells you how much the values differ from the mean of 7, which in this case is approximately 2.83.
    The MTT assay is a colorimetric assay for assessing cell metabolic activity. It is widely used to measure cell proliferation, cytotoxicity, and cell viability.
    The MTT assay is based on the reduction of the yellow tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to purple formazan crystals by metabolically active cells. This reduction occurs in the mitochondria of living cells and indicates cell viability.

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