Hi. To measure hydrophobicity with FLIM you need a dye with different quantum yield in water compared to hydrophobic media. You can google "NBD dye". Some infos you can also find in our application note on FLIM in Confocal Microscopy Applications: www.picoquant.com/dl_technotes/AppNote_FLIM_2012.pdf. Please feel free to contact our support team directly with any questions at support(at)picoquant.com
HI, great presentation. Please could you send through/mention a reference of how FLIM is used to measure hydrophobicity? Thank you in advance. Best.
Hi. To measure hydrophobicity with FLIM you need a dye with different quantum yield in water compared to hydrophobic media. You can google "NBD dye". Some infos you can also find in our application note on FLIM in Confocal Microscopy Applications: www.picoquant.com/dl_technotes/AppNote_FLIM_2012.pdf.
Please feel free to contact our support team directly with any questions at support(at)picoquant.com