Hi Caroline. Sorry that I'm just seeing this comment now. You should be able to access the files through this link: www.dropbox.com/sh/gj91r0vj5d8biv4/AADSHJyDRHnjxakN5zn2Fp_2a?dl=0
Thank you very much for sharing, but please can i get a link to the files to be downloaded. i typed the link(displayed in 2:17) to the file in google search and box responce was "This shared file or folder link has been removed or is unavailable to you." Please how can i get the file so i can follow the tutorial.
Thanks for the video but at around 44:30 the table where you explained Logical Operators, shouldn't the x < 5 be "False", rather than "True" as shown in the table?
@hwbtafrsca Hi Dr Stephen Guest, I am a RA for CRISPR library screening in the lab. however, I have no bioinformatics programming background. Our lab has bioinformatics post-docs that analyze datas, but I would like pick up the bioinformatics skills. I would like to learn how to analyze the datas from the fastq files. Can you please advise how and where should I learn R programming for the CRISPR library screening? Thank you.
If you want to start your analysis from fastq files you will need to become familiar with working from the command line. You could start to build those skills by taking the Command Line Tools for Genomic Data Science course on Coursera. Once the fastq files have been processed you should be able to use R to perform the rest of the analysis. There are a lot of great resources out there for continuing to learn R beyond these videos e.g. the Foundations Using R course on Coursera. Additionally, there may be specific software that has been developed for analyzing data generated by the CRISPR library that you are using, so make sure to check with the creators of the library to see if there is specific software that they recommend. Good luck!
5:00 what a strange mindset. Not a single craftsman, not a single man with elementary school education would say such a nonsense. Only in academia. No wonder the entire academia went down a sink
It's actually a very widely held attitude when it comes to anything computer related - did you buy the browser you are using? Or the email software you use? There is an expectation of "free" that is VERY widely held. Also, it should be noted that the people/companies that make some of these propriety software effectively priced themselves completely out the market - we are usually not talking about a few dollars, but rather hundreds even thousands of dollars per year.... why would you spend that amount of money when a free alternative exists?
@@unlearningcommunism4742I was in academia from 1927-2034. Please stop with your bullshit. I’m glad you aren’t, and NEVER WAS in academia. The last thing research institutions need is for someone as fundamentally messed up as you to pollute the research environment. We get that your smarts and intelligence don’t quite meet the standard, hence you resorting to ranting on youtube videos that have nothing to do with you, but please, no one wants to see or hear about what you have to say.
Thanks for posting this! I'm afraid that the tutorial files are no longer accessible.. It'd be massively helpful if you can upload them again please?
Hi Caroline. Sorry that I'm just seeing this comment now. You should be able to access the files through this link: www.dropbox.com/sh/gj91r0vj5d8biv4/AADSHJyDRHnjxakN5zn2Fp_2a?dl=0
Very much thank for posting this! It really helps!!
Thank you very much for sharing, but please can i get a link to the files to be downloaded. i typed the link(displayed in 2:17) to the file in google search and box responce was "This shared file or folder link has been removed or is unavailable to you." Please how can i get the file so i can follow the tutorial.
Files can be found here: drive.google.com/drive/folders/1mOCELXFb-b91C9mvfb2zD9nUTvNqqihO?usp=share_link
Thanks for the video but at around 44:30 the table where you explained Logical Operators, shouldn't the x < 5 be "False", rather than "True" as shown in the table?
Yes! Absolutely correct. Nice catch.
"difficultoread" got me lmao😂
thank you!
@hwbtafrsca Hi Dr Stephen Guest,
I am a RA for CRISPR library screening in the lab. however, I have no bioinformatics programming background. Our lab has bioinformatics post-docs that analyze datas, but I would like pick up the bioinformatics skills. I would like to learn how to analyze the datas from the fastq files. Can you please advise how and where should I learn R programming for the CRISPR library screening?
Thank you.
If you want to start your analysis from fastq files you will need to become familiar with working from the command line. You could start to build those skills by taking the Command Line Tools for Genomic Data Science course on Coursera. Once the fastq files have been processed you should be able to use R to perform the rest of the analysis. There are a lot of great resources out there for continuing to learn R beyond these videos e.g. the Foundations Using R course on Coursera. Additionally, there may be specific software that has been developed for analyzing data generated by the CRISPR library that you are using, so make sure to check with the creators of the library to see if there is specific software that they recommend. Good luck!
5:00 what a strange mindset. Not a single craftsman, not a single man with elementary school education would say such a nonsense. Only in academia. No wonder the entire academia went down a sink
It's actually a very widely held attitude when it comes to anything computer related - did you buy the browser you are using? Or the email software you use? There is an expectation of "free" that is VERY widely held.
Also, it should be noted that the people/companies that make some of these propriety software effectively priced themselves completely out the market - we are usually not talking about a few dollars, but rather hundreds even thousands of dollars per year.... why would you spend that amount of money when a free alternative exists?
Are you even in academia?
@@natalias374 Of course not. Why would I stay in that hell-hole? I was in academia from 2010 to 2016. I would rather work in a Victorian coalmine
@@unlearningcommunism4742I was in academia from 1927-2034. Please stop with your bullshit. I’m glad you aren’t, and NEVER WAS in academia. The last thing research institutions need is for someone as fundamentally messed up as you to pollute the research environment. We get that your smarts and intelligence don’t quite meet the standard, hence you resorting to ranting on youtube videos that have nothing to do with you, but please, no one wants to see or hear about what you have to say.