Helpful video, but NOT WEARING GLOVES WHEN HANDLING THE GEL MOST OF THE TIME! I'm not usually hung up on safety stuff, but in this case, liquid acrylamide is a neurotoxin, and exposure can lead to progressive peripheral neuropathy. You can never be sure that the gels are 100% polymerized. Kids - don’t do this stuff without a lab coat, safety goggles, and at least vinyl gloves.
I still don't understand why it's not allowed to have inner buffer leak to the outer buffer in this video. It's appropriate to overfill inner chamber and have inner buffer leak out in my lab and so is some protocols.
you are allowed that is to say, it'll work, im not sure if ALWAYS, but in the youtube videos put out by the bio rad manufacturers the electrophoresis demonstration involves overflow, I'm not sure if it was SDS though
You need to maintain constant flooding of the fluid on the gel, the fluid actually has different pH gradients from the outside buffer. If you have a leak you risk equilibriating the volumes and getting a dry gel, this can mess up the results. But even if it isnt a full seal it will still work as long as the gel remains flooded with buffer.
This video saved my thesis work back in 2014 and 2015. Thank you
A very explanatory step-wise tutor. Thanks so much
Thanks for assisting with my crisis after lab members went home :D
Comprehensive and clear explanation. Thank you
Helpful video, but NOT WEARING GLOVES WHEN HANDLING THE GEL MOST OF THE TIME!
I'm not usually hung up on safety stuff, but in this case, liquid acrylamide is a neurotoxin, and exposure can lead to progressive peripheral neuropathy. You can never be sure that the gels are 100% polymerized.
Kids - don’t do this stuff without a lab coat, safety goggles, and at least vinyl gloves.
Thank you for giving us the video version of detailed steps much better than reading texts that is so difficult to understand.
Very professional explanation
So nicely explained, I give this to my project students before they enter the lab :) !
I felt like I was listening to my gearhead garage dad explain how to change the oil XD
You know what this is the latest advanced equipment that we have bought in my university
Thanks for this video........keep it up.......
What a constructive presentation
Wonderful Video
Thanks sir
Thanks for upload this useful video
Great video! Thanks for sharing!
awesome
Great video! Very insightful.
9:00 Don't panic,reload about 1mm between the space
Of well
awesome.. one of the best
There are 3 types of SDS PAGE, What's the difference between the 3 types?
1. Native SDS PAGE
2. Reducing SDS PAGE
3. Nonreducing SDS PAGE
IS NOT A TEST SIR
LOL @ 7:21
oh gosh, love it when that happens
he didnt don gloves
Talk about precision.... ;-)
what is the advantage of choosing constant voltage over constant current?
i have a doubt . should the plates go in the space behind the ears or should they sit above the ears in the electrode assembly unit.
Thank you! This is extremely helpful
Helpful indeed!
I still don't understand why it's not allowed to have inner buffer leak to the outer buffer in this video. It's appropriate to overfill inner chamber and have inner buffer leak out in my lab and so is some protocols.
you are allowed that is to say, it'll work, im not sure if ALWAYS, but in the youtube videos put out by the bio rad manufacturers the electrophoresis demonstration involves overflow, I'm not sure if it was SDS though
You need to maintain constant flooding of the fluid on the gel, the fluid actually has different pH gradients from the outside buffer. If you have a leak you risk equilibriating the volumes and getting a dry gel, this can mess up the results. But even if it isnt a full seal it will still work as long as the gel remains flooded with buffer.
Thanks for this great video!
Nice instruction - but, why are you not wearing gloves!!
WHEN DID YOU ADD THE GEL?
He used a gel that its ready to go.
Thank you for the video! It was really helpful :)
its good
Тhank you!
hhhhhm neurotoxins... wear your gloves kiddos
08:13 That's a good load. Lol
This is mine project
vietsub,please!