Helpful video, but NOT WEARING GLOVES WHEN HANDLING THE GEL MOST OF THE TIME! I'm not usually hung up on safety stuff, but in this case, liquid acrylamide is a neurotoxin, and exposure can lead to progressive peripheral neuropathy. You can never be sure that the gels are 100% polymerized. Kids - don’t do this stuff without a lab coat, safety goggles, and at least vinyl gloves.
I still don't understand why it's not allowed to have inner buffer leak to the outer buffer in this video. It's appropriate to overfill inner chamber and have inner buffer leak out in my lab and so is some protocols.
you are allowed that is to say, it'll work, im not sure if ALWAYS, but in the youtube videos put out by the bio rad manufacturers the electrophoresis demonstration involves overflow, I'm not sure if it was SDS though
You need to maintain constant flooding of the fluid on the gel, the fluid actually has different pH gradients from the outside buffer. If you have a leak you risk equilibriating the volumes and getting a dry gel, this can mess up the results. But even if it isnt a full seal it will still work as long as the gel remains flooded with buffer.
A very explanatory step-wise tutor. Thanks so much
Thanks for assisting with my crisis after lab members went home :D
Helpful video, but NOT WEARING GLOVES WHEN HANDLING THE GEL MOST OF THE TIME!
I'm not usually hung up on safety stuff, but in this case, liquid acrylamide is a neurotoxin, and exposure can lead to progressive peripheral neuropathy. You can never be sure that the gels are 100% polymerized.
Kids - don’t do this stuff without a lab coat, safety goggles, and at least vinyl gloves.
Comprehensive and clear explanation. Thank you
So nicely explained, I give this to my project students before they enter the lab :) !
I felt like I was listening to my gearhead garage dad explain how to change the oil XD
Thank you for giving us the video version of detailed steps much better than reading texts that is so difficult to understand.
Great video! Thanks for sharing!
LOL @ 7:21
oh gosh, love it when that happens
You know what this is the latest advanced equipment that we have bought in my university
Very professional explanation
Great video! Very insightful.
Thank you! This is extremely helpful
Thanks for this video........keep it up.......
9:00 Don't panic,reload about 1mm between the space
Of well
What a constructive presentation
Thanks for upload this useful video
Thanks for this great video!
Talk about precision.... ;-)
awesome.. one of the best
Wonderful Video
There are 3 types of SDS PAGE, What's the difference between the 3 types?
1. Native SDS PAGE
2. Reducing SDS PAGE
3. Nonreducing SDS PAGE
IS NOT A TEST SIR
i have a doubt . should the plates go in the space behind the ears or should they sit above the ears in the electrode assembly unit.
Thank you for the video! It was really helpful :)
Thanks sir
what is the advantage of choosing constant voltage over constant current?
Helpful indeed!
he didnt don gloves
awesome
Nice instruction - but, why are you not wearing gloves!!
I still don't understand why it's not allowed to have inner buffer leak to the outer buffer in this video. It's appropriate to overfill inner chamber and have inner buffer leak out in my lab and so is some protocols.
you are allowed that is to say, it'll work, im not sure if ALWAYS, but in the youtube videos put out by the bio rad manufacturers the electrophoresis demonstration involves overflow, I'm not sure if it was SDS though
You need to maintain constant flooding of the fluid on the gel, the fluid actually has different pH gradients from the outside buffer. If you have a leak you risk equilibriating the volumes and getting a dry gel, this can mess up the results. But even if it isnt a full seal it will still work as long as the gel remains flooded with buffer.
Тhank you!
WHEN DID YOU ADD THE GEL?
He used a gel that its ready to go.
hhhhhm neurotoxins... wear your gloves kiddos
its good
08:13 That's a good load. Lol
This is mine project
vietsub,please!