I don’t know if you still answer questions on here, but I’m having an issue once I finish calibration. Upon measuring an image, the max value fir OD shoots super high (285521.668, to be exact). I think its because the MGV fir that data is equal to 0, and the function goes exponential. Is there a way to fix this so that it doesn’t deviate so greatly?
Thank you very much for the video, it is very helpful. Sorry can you please the reference to use it for publication. I also would like to know if you could show us how to do cumulative threshold spectra, it would be wonderful. Thank you again
The method was very simple and great. I am using it to measure protein expression in DAB stained immunohistochemical slides. But, can you please provide any reference of a published article that used this method? It would be really helpful. Thanks.
Can you tell please, how to know the optical density for mast cells (stained with toluidine blue) in order to classify them in different types according to their staining intensity
Hello! Thanks for this very helpful video! But I have one question. I obviously got different mean gray values in my picture than yours, so how can i find the known OD values to calibrate my image??
Hello. Thank you for this very helpful tutorial. I have a few questions: Why every time I open ImageJ and do the calibration of step tablet image I get different mean gray values? Why even though I take the same mean gray values as previous measures with the corresponding O.D I get every time different numbers in the calibration curve formula?
hello thanks for this video, i have a question. I want to quantify IHC in the aorta can I quantify the intensity of staining too by this method? I am comparing the protein expressions but I don't know how to do measurements.
@@jinaltrivedi90 is there any option to share my image to you so that you can guide me on how to do. i am very new with this software and trying to learn by my own
Thankyou so much for this tutorial, it helps me a lot on my final task~~~
You are welcome
Indeed Indians are best
thank you so much sir, really helpful.
video really hepls me, i could understand very easy. Thanks!!
I don’t know if you still answer questions on here, but I’m having an issue once I finish calibration. Upon measuring an image, the max value fir OD shoots super high (285521.668, to be exact). I think its because the MGV fir that data is equal to 0, and the function goes exponential. Is there a way to fix this so that it doesn’t deviate so greatly?
It was very useful tutorial
Thank you very much for the video, it is very helpful. Sorry can you please the reference to use it for publication. I also would like to know if you could show us how to do cumulative threshold spectra, it would be wonderful. Thank you again
The method was very simple and great. I am using it to measure protein expression in DAB stained immunohistochemical slides. But, can you please provide any reference of a published article that used this method? It would be really helpful. Thanks.
I already given reference in comment above .if u can t find let me know I will send u...thank u for your appriciation
thank you very very much for this video ,please can you tell me how can i measure the diametre of one particule with image j
Can you tell please, how to know the optical density for mast cells (stained with toluidine blue) in order to classify them in different types according to their staining intensity
You just need to convert your image into grey scale...so that your stain will be dark black which you can measure
@@jinaltrivedi90 thank you very much! 🙏🏻
@@Alex-gw6pm hope that would be helpful let me know
Hello! Thanks for this very helpful video! But I have one question. I obviously got different mean gray values in my picture than yours, so how can i find the known OD values to calibrate my image??
imagej.nih.gov/ij/docs/examples/calibration/#:~:text=Analyze%2FCalibrate%20dialog%20box.,%2C%20then%20press%20%22OK%22.
Please check above link...if not useful let me know
Hello. Thank you for this very helpful tutorial. I have a few questions: Why every time I open ImageJ and do the calibration of step tablet image I get different mean gray values? Why even though I take the same mean gray values as previous measures with the corresponding O.D I get every time different numbers in the calibration curve formula?
may due to minor difference in the selection of area......i haven't faced that kind of problem...
what if my IHC picture has both H and DAB brown? what should I do to calculate OD? is there a easy plug in?
no there is no any plug in....but u have to select the region
where i can show up the step table?
Before calibrating we need to open the image ? I cant get this point
Hello, do you know if I need do this kind of calibration to measure the grayscale of muscles? Or just the measure scale is enough?
Thanks
For muscle Echogenicity
This scale is enough
hello thanks for this video, i have a question. I want to quantify IHC in the aorta can I quantify the intensity of staining too by this method? I am comparing the protein expressions but I don't know how to do measurements.
khan asad ya u can but u have to minus background noise
@@jinaltrivedi90 is there any option to share my image to you so that you can guide me on how to do. i am very new with this software and trying to learn by my own
@@Positive_chapati_vibes yup u can mail me your image @jinaltrivedi90@gmail.com
@@jinaltrivedi90 thanks very much check your email
Is it possible for me to measure the density of shirt stains with this? Please answer quick I need major help
May be I m not sure
References, please
imagej.nih.gov/ij/docs/examples/calibration/
www.researchgate.net/post/How_do_I_calculate_the_optical_density_of_a_selected_portion_of_an_image_on_ImageJ