Microscopy: Super-Resolution: Overview and Stimulated Emission Depletion (STED) (Stefan Hell)
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- Опубликовано: 1 окт 2024
- Learn more: www.ibiology.o...
Historically, light microscopy has been limited in its ability to resolve closely spaced objects, with the best microscopes only able to resolve objects separated by 200 nm or more. This limit is know as the diffraction limit. In the last twenty years, a number of techniques have been developed that allow resolution beyond the diffraction limit. Here, Stefan Hell, who invented many of these techniques, gives an introduction to these super-resolution microscopy techniques, and a detailed discussion of two such techniques: STED (Stimulated Emission Depletion) and RESOLFT (REversible Saturable OpticaL Fluorescence Transitions). 
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Thank you for such a great overview of STED microscopy!! Easy to understand explanation and a well made video.
He invented it. So, he knows the stuff too well to deconstruct it down to the basics.
I checked every website that explains how STED works with no success and saw this video but didn't watch because it was too long for me. After three hours of time wasting, i'm finally here and learning from the inventor. Thank you sir.
Ikr. For the sake of time, I even went and skimmed through his 2008 paper, but in the end, I came back here and watched it in full.
Just watch it bro it's not that long considering the whole picture
What never becomes clear in this type of explanations of STED is: as is it is not possible to produce a smaller spot of light than about 0,5 wavelength in diameter, why is it possible to make an unlit spot smaller thsn that?
+1 also didn't get it although it seems to be the key point
same for me was asking myself that all the time
It's the diffraction pattern. When creating a "donut" diffraction pattern, the thickness of the wall of the donut are still limited and set by the diffraction limit but you can change the size of the donut. So you can make the hole of the donut much smaller than the walls.
congratulations dr. hell for thenobel prize
another good reason for loving donuts. Thank you.
I feel honnered to watch a lecture by this great man!
Breaking theoretical limits, very cool!
Theoretical limits cannot be broken and no such thing happened here.
I think bypassing instead of breaking is a more correct way to describe it
hard to think, while the green is limited to 200 nm the red light that is larger in WL can be eliminated in the small circle that is much smaller than the WL of red. i would expect that diffraction of red light make the small circle useless. confusing!
I don't understand. Can you rephrase?
Thank you a lot! I have to prepare a exam about different microscopy methods and the chapter about STED was really unclear... just some images and a bunch of formulas! You managed to explain the basics in 5 mins!
Thanks for clearing out the confusion about the tech. I would like to use parts of the video for my presentation, if thats okay with the educational purposes.
Super educational thank you for this lovely video I just came across of it, I wonder if it would help in cytometry, We are going to do a series of experiment what are high speed camera we should definitely try to keep this in mind.
The Lucas, Graton-Dane and Rife Microscopes are discussed in this article by Seidel and Winter in the Journal of the Franklin Institute:
web.archive.org/web/20020811133526/www.navi.net/~rsc/seidel.htm
There is also interesting work by Gaston Naessens as well on various sites worth perusing.
Not to mention the incredible Multiple Radiation Microscope of Elmer Pierre Nemes.
sehr sehr gut verständlich! endlich etwas ausführlicheres. Danke :-)
How do neurons, neo-genic, get from the rotary gyrus (places of production) to their destination? The pulsations seem para-systolic and seem likely to me that it's not only bioelectric signals being relayed
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Indeed it's a good lecture about the great work he did.
thnk u so much .. it was really helpful :)
This was a real eye-opener for me.
awesome work.great help
Amazing. Thank you so much
Yes ibiology
What is the cost of a STED microscope?
Wow. amazing lecture
Wo sind denn die deutschen Videos?
Auch finde ich keine Videos in Deutsch von Prof.Clemens Kaminski(Super resolution)
Great explanation! Very clear!
this teacher is amazing. teaching step by step in simple ways with smooth expression
He is the winner of the Nobel Prize for his invention of STED microscopes :)
21:55 Different states other than on/off.
Beautiful the video
Amazing lecture. I truly enjoyed it.
Does the sample to be irradiated by laser have to be fluorescent?
@Rijkaard And you are a troll.
@Anna Pamela de Jesus Fluorescent is not necessary, technically for RESOLFT. You can use any quantifiable property which can be turned on and off in time with nanometer precision.
Stefan Hell, the Nobel Price winner in Chemistry in 2014, has been born in Romania.
Flavius Aspra You need a very high resolution to see a difference.