[@MuhmmedNadeem-cy2tj]

Dude, from all those weird Indians making subjects worse on RUclips, never thought there would be one who could help me. Till I found your channel.
+1Like
You are awesome mate!!
Thanks alot!

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@albertmontes11]

Thank you. You gave me a basic understanding of monoclonal process creating proteins. Keep instructing!!!

[@shomusbiologyofficial]

Thank you

[@valeriehollstein5356]

all your tutorials I have viewed from you are very well-explained. Thanks a bunch!

[@uncannyskrib937]

My PI is investigating the use of a recombinant hACE2 protein as a potential delivery system of anti-SARS-CoV-2 drugs and I used this video as a refresher to help write a summary paper for this idea. Just last semester I was using your videos to study for my final exams, and now I'm using them to potentially help others develop a treatment for the novel coronavirus. Thank you!

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@shirasagi9390]

love your videos. every time i dont understand something i watch your channel and it becomes crystal clear. thank you so much

[@shomusbiologyofficial]

Glad to hear that you're getting benefit from my lectures. Please subscribe and share

[@eslamyousef6131]

your a legend bro doing everyone a massive favor, keep up the hard work

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@zoypuff]

Thanks, very helpful and simple to understand

[@tb2748]

could you make a video on what fusion protein is? and on what protein/affinity tags are? your lectures are very helpful!

[@saraassar5264]

Very informative. I liked your energy!

[@shomusbiologyofficial]

Thank you

[@gangasasikumar2283]

Thank you sir. It was amazing. Im watching this on the day before my examination.. It makes me cool.. Thank you so much sir.. Keep going ❤️

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@maroonhorizon1693]

WONDERFULLLLL! ABSOLUTLEY AMAZINGGGG

[@shomusbiologyofficial]

Thank you

[@TheRockstar-gk9xp]

Thanks Shomu ,God Bless You

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@vishwaspethe3275]

Very clear explanation of what a recombinant protein is. Thank you for publishing the video.

[@noorulain7302]

Greatly helpful....... Thanks for your efforts bravo

[@shomusbiologyofficial]

You're welcome

[@deekshithasubrahmanian5645]

Sir You are really amazing, and its really helpfull your videos to study , thank you somuch

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures and

[@dakota1432]

I needed to learn a basic understanding of plasmids and this video was beyond helpful. Keep it up man, if you need help with marketing videos let me know.

[@RoyBattyLives]

Thank you Shomu, you are a great teacher

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@foggedgoggles]

Thank you so much your videos are so helpful!

[@shomusbiologyofficial]

+Sharon Joseph thank you. Glad you liked my lectures

[@sajidahussain7406]

Thank you sir.. Your way of delivery is so easy and understandable by us easily

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@sajidahussain7406]

@@shomusbiologyofficial you deserve it sir

[@keshavsingh2078]

Very helpful . Thank you

[@johnloubbardi1034]

very helpful explanation video. Thank you

[@shomusbiologyofficial]

You're welcome

[@alondradelgado4630]

What a great video! Thank you, Shomu

[@shomusbiologyofficial]

Thank you

[@zamilkisekka7701]

Great stuff. U made it look very simple

[@shomusbiologyofficial]

Thank you so much for appreciating my efforts

[@qudratfikrat2023]

Very well explained, thank you!

[@shomusbiologyofficial]

You're welcome

[@jagrityesingh4940]

u ve excellent teaching style.. its very helpful....

[@shomusbiologyofficial]

+jagritye singh thank you. Glad you liked my lectures

[@abirhasanpranto7593]

👌👌

[@deechan623]

I love this. Thank you so much!!!

[@shomusbiologyofficial]

You're welcome

[@sumanthota7356]

Thank You so much for your wonderful explanation 👌👍👏

[@shomusbiologyofficial]

You're welcome. Glad you liked it

[@arulexport2701]

Wow what a simple great explanation ''' such a huge topic every one easily understood thank u sir

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@Hipnot]

Thanks a lot ! Nice and clear explanation !

[@shomusbiologyofficial]

You're welcome

[@lobbes44]

This was really really good explained, i now can pass my exam!

[@shomusbiologyofficial]

All the best

[@iffathtaskeen9332]

sir.. The best explaination...
Thanks sir....

[@shomusbiologyofficial]

You're welcome

[@mayukhroy5393]

Thank You Sir, this video was very helpful.

[@shomusbiologyofficial]

You're welcome. Glad to hear that you're getting benefit from my lectures

[@bornitadeb8282]

Awesome...just amazing

[@shomusbiologyofficial]

Thank you

[@miguelguerreiro1212]

You saved the day! much better than my teacher

[@ignatiousramaano1636]

Well explained🙌🏾

[@shomusbiologyofficial]

Thank you

[@mercedesgarciacarrillo1736]

Love this channel 🧡

[@shomusbiologyofficial]

Thank you. Glad you liked my lectures

[@nishi782]

awssmmm teaching style and easily understandable lecture

[@shomusbiologyofficial]

+Swarnika Kumari thank you. Glad you liked my lectures

[@heydariparisa1756]

very well explained. Thank you very much

[@shomusbiologyofficial]

+Heydari Parisa thank you. Glad you liked my lectures

[@mayukhroy5393]

Thank You Sir, for this video.

[@shomusbiologyofficial]

You're welcome

[@nickynicky8184]

Very useful for mee 🙏🏼🙏🏼🙏🏼

[@shomusbiologyofficial]

You're welcome

[@anastasia9370]

thank you so much for this

[@shomusbiologyofficial]

You're welcome

[@ithirstyforknowledge]

Nicely explained. A question: the recombinant plasmid will multiply to generate more similar or without the gene? And why?

[@jyotiraj9362]

sir please make a video of strain improvement

[@shomusbiologyofficial]

+Jyoti Raj okay. I will Keppra that in mind

[@chintanmehta4695]

Superb sir.

[@shomusbiologyofficial]

Thank you

[@geraldomedeiros5081]

Excelent video!!! By the way, what is the gene that encodes human insulin for type 1 diabetes?

[@shomusbiologyofficial]

INS gene located on the short arm of human chromosome no. 11 at position 15.5

[@shscompany1188]

you're our big hero

[@shomusbiologyofficial]

+shs company glad I can help

[@naveenantony7849]

9:00 if the plasmid is capable of self-replicating itself and provide us with multiple copies of the "gene of interest" why should we bother to insert the plasmid into an Ecoli bacteria??

[@sangeetadevi5819]

Plasmids are extra-chromosomal ,self replicating ,double stranded circular DNA molecules ...u know that ?! Ryt. So ,it would replicate when it is in the cell ,...

[@naveenantony7849]

@@sangeetadevi5819 i appreciate your reply👍🏼,but what is the purpose of inserting the plasmid to a bacteria (Ecoli here)..

[@sangeetadevi5819]

This is the whole biotech is all about !! Using microbes , plant/animal cells / their parts(maybe enzymes also) to get the products useful for human beings .🙃🙃

[@sangeetadevi5819]

The bacteria (E.coli ..in this particular case) ,the plasmid DNA ..all are used to produce the products we require.

[@techheaven007]

because it is only capable to replicate inside E.coli..and it requires the cell machinery to replicate ! if we take a shuttle vector that works both in both E.coli and an eukaryotic cell like yeast then we can insert that recombinant plasmid to the yeast also ! its depending on the protein you want to get.. if its an animal or human protein that needs post transcriptional modification, you have to transfer the vector to an eukaryotic cell otherwise a prokaryotic cell is fine..

[@crownofall]

I feel i need more details so I'll look at your other vids.

[@lubabahnuman3227]

great job

[@shomusbiologyofficial]

Thank you

[@saiky2925]

Thanks, legend

[@shomusbiologyofficial]

You're welcome

[@aayushisingh5651]

Thankyou sir❤

[@shomusbiologyofficial]

You're welcome

[@Moongrace-sj4fc]

You are a blessing shomu . Please make a video on production of biomass and primary, secondary metabolites please🥰🥰

[@shomusbiologyofficial]

Okay. Thank you

[@Moongrace-sj4fc]

@@shomusbiologyofficial your welcome 😄

[@martinaschembri7933]

thank you so much!

[@ashokapalihawadana6407]

thank you so much

[@shomusbiologyofficial]

You're welcome

[@hamedhosseini4938]

Please make a video about GMO's.

[@shomusbiologyofficial]

+Hamed H I will make that soon.

[@hamedhosseini4938]

Ty

[@hamedhosseini4938]

Bro where is GMO? ;n;

[@itsnotme8554]

HIV test kit have pre-coated antigens. So during the testing means pouring the blood drop from finger to test kit if we touch the dropper end to the test kit so can antigen go to our body and can give infection?

[@aynuraydin6991]

Thank you for your video!!!

[@shomusbiologyofficial]

You're welcome

[@RohitKhajuriaa]

awesome out of the world

[@patriciajimenez7443]

Hi, what is the process for extracting these recombinant proteins from the E.coli cell?

[@patriciajimenez7443]

Also, thanks for this video. This one is particular has been so helpful in so many levels!

[@crownofall]

I need this answer also.

[@HelloAayush]

Downstreaming is purification and separation process.

[@khaledalharmoudi3350]

Thank you. helped me a lot.

[@shomusbiologyofficial]

You're welcome

[@MANISHKUMAR-rz5fv]

Please upload the video on protine engineering 🙏🙏

[@shomusbiologyofficial]

Sure

[@mariyalexander]

Just simply loved it ❤

[@shomusbiologyofficial]

Thank you

[@prashantkumar409]

Shomu make a vedio on fusion protein.... plzzz

[@shomusbiologyofficial]

Okay

[@deepikak.p5903]

Sir can you say about heterologous protein in brief .

[@1307paolo]

Thanks!

[@farhanakhatoon26]

Thanku so much!😃

[@shomusbiologyofficial]

+Farhana Khatoon glad you liked it

[@cvhashim]

Thanks man

[@shomusbiologyofficial]

+cvhashim thank you

[@apurva2171]

hello ,
i got a question somewhere
why is recombinant protein cloned before tag sequencing ?

[@markboccanfuso2871]

So this can be used for bad as well as good

[@BE74297]

ya more creepy control science, cause of all the problems

[@jyotiraj9362]

sir after 3 days my exam will be started so plz make this video....

[@Lumax96]

Hi, I've just got a question to see if I understand: so the DNA that is being used is called recombinant DNA because its different from the naturally occuring DNA, namely because its a recombination of a bacterial plasmid and a eukaryotic gene. But the protein that is being produced this way does not differ from the naturally occuring protein, but is called recombinant protein because it was produced using the recombinant DNA. Is that correct?

[@techheaven007]

yes

[@NH-jl2nb]

very nice

[@shomusbiologyofficial]

Thank you

[@ganguliify]

great.....keep it up

[@MomoTV]

thank you

[@lyndabelhadi7787]

Thanks

[@shomusbiologyofficial]

You're welcome

[@divyaarora_5681]

Is it same as chimeric proteins??

[@rajeshr4115]

What is the difference between Biologics and biosimillar production one video upload bro

[@zahrabatool7761]

Please give detail video

[@thedevilsadvocate5210]

How do they collect the desired protein?

[@nunitchagucci3047]

You are awesome! Always! :)

[@shomusbiologyofficial]

+nunitcha gucci thank you.

[@lovepunjab5429]

As we know plasmid is undergoes self replication , then why we place it into bacterial cell . Why we cannot give some chemical to it out side thy body _in laboratory??

[@abbasi1907]

Recombinant antigens are virulent or non virulents ?

[@Naveed_14]

Recombinant Protein Expression in E. coli (Inducible Promoter System)

[@shomusbiologyofficial]

Okay

[@ShubhamSingh-gr3is]

How recombinant proteins is recovered from host cell

[@leroyjenkins3375]

But how is the Plasmid inserted into the Bacterial Cell??

[@muthyalakapil8743]

Inserted with the help some physical methods like micro injection electroporation, some chemicals

[@muthyalakapil8743]

Thank you so much sir... please make a video on chromosome jumping.. please sir ..

[@shomusbiologyofficial]

Will make it soon

[@chem337]

❤❤❤

[@shomusbiologyofficial]

Thank you

[@HelloAayush]

👍

[@Nhthuyanh]

Hoping my biotech final will go well

[@shomusbiologyofficial]

All the best for the exam

[@susanazizi3890]

In german we say : bester Mann

[@Patelpawancool]

Your video's super. But. I think. At last the video u summerise whole video in few Ward's. Do this reason user get easily. Motive your videos

[@shomusbiologyofficial]

That's a good idea

[@Swatikapoor...]

Is this also called protein engineering

[@shomusbiologyofficial]

Yes

[@deepalipandey829]

You look so good😍❤️

[@shomusbiologyofficial]

Thanks

[@nishakashyap1352]

🙂thanku sir

[@shomusbiologyofficial]

You're welcome

[@rijilfreekdaredevil]

Thank you bro🌹

[@shomusbiologyofficial]

You're welcome

[@ashutoshthakur7712]

Bhai sahab...Angreji achi hai....Nhi Nhi. bohot achi hai... please thoda biology ka bhi content badhaiye....Baaki aapk angreji ka assent acha hai

[@shomusbiologyofficial]

Biology ka content kam laga??

[@wasimsalafi]

And now because of "go rakshaks" one cannot count on cows to extarct recombinant insulin in India ;)

[@shomusbiologyofficial]

We no longer use cow. We use bacteria now. It's much better and faster. Use recombinat dna technology

[@wasimsalafi]

@@shomusbiologyofficial hi Shomu, in case you didn't get my joke, i was kidding. Btw nice explanation of cloning.

[@kamranhaider5108]

We tolerate u in ur videos

[@fadhlalshaebi2314]

Thanks

[@shomusbiologyofficial]

You're welcome

[@fadhlalshaebi2314]

i need recombinant the Il-34 protein and amplification it in the Lab. i ask if its possible. Thank so much