Cell Culture Guide: Mycoplasma Contamination in Cell Culture [Full Version]

Mycoplasma is the nightmare of every scientist working with Cell Culture. Mycoplasma contamination is threatening academic labs to biopharmaceutical production facilities.
Dr. Rüdiger Arnold, one of our PromoCell Academy lecturer, explains why best practice in detection, elimination and avoiding mycoplasma are necessary for your cell culture.
► To know more on PromoCell products for mycoplasma detection and elimination: www.promocell.com/portfolio/m...
---
Follow us on social media:
► LinkedIn: / promocellgmbh
► Facebook: / promocellgmbh
► Twitter: / promocell_gmbh
---
At PromoCell, we help scientists do better research with a world-class portfolio of human primary, stem and blood cells as well as optimized cell culture media. With over 30 years of expertise, we are recognized globally for supplying scientists with the tools and support they need to do groundbreaking research.
Your science is moving the world forward - and we’re here to help.

[@funny11744]

Question : DOES THE MICOPLASMA PENETRATES THROUGH THE BODY SKIN ( HANDS, FOOT) COULD REACH IN THE BLOOD ?

[@gorilachachacha]

Are the sequences of the primers available?

[@jonsasa8646]

How do you prevent Mycoplasma from cell culture?

[@johannaweh8196]

i read that the mycoplasma are in ne membran of the cells.
so.. are there enough in the media surround or is it better to take cell suspension ( when you have adharent cells)?

[@PromoCellGmbH]

Hi Johanna, the vast majority of mycoplasma species are located extracellularly, i.e. outside the cell. This is also true for the mycoplasmas that are normally found in cell cultures. For PCR-based detection of mycoplasmas in cell cultures, culture supernatants have proven to be a very suitable sample material. We hope it helps :)

[@xanderz161]

If mycoplasma is such a problem in cell cultures, then what do you think it's doing to us? Has anyone asked that question?

[@gigimatz9222]

great question. They might just be in a mutualistic or commensal relationship with us. However, if it is parasitic, then major health issues would've been found a long time ago.

[@xanderz161]

Let's see. Pneumonia, STDs, arthritis, skin lesions, and multiple immune issues. These are just some of the known. It also depends on the strain. The incognitus strain being the most severe. It was discovered in 1987, in AIDS patients.

[@gigimatz9222]

@@xanderz161 interesting perspective! Thank you for the insight!

[@xanderz161]

@@gigimatz9222 You're welcome.