Hello, thank you for the great video. I am a non biologist trying to teach myself ngs analysis. I have 2 questions that might have obvious answers however I am not able to understand: 1) why to bother with "a base and color define the next base" when one can use 16 colors instead of 4 ? 2) what is the use of the 3 middle universal bases in the probes? i.e why not just ligate all di-bases at one cycle (instead of the offset 5 times...) and decode the colors ? thanks in advance!
hello Thanks for the excellent video. Could you please clarify something? The fragments of all unique DNA sequences are flanked by the P1 and P2 primers. Hence when they are binding to the beads, how is it ensured that each bead carries unique DNA sequences. These interactions are with the adaptor sequences right? Hence how is this possible since all DNA fragments carry same adaptor sequences?Thanks.
There are different adaptors for different fragments of DNA. The beads carrying DNA sequences(primers/ oligonucleotides/amplicons)are made by emulsion PCR and one bead is made in such a way that all the primers attached to it carry sequences complementary to any one type of adaptor, among many others. So, suppose there are 10 types of adaptors used to flank the fragmented DNA, there would be 10 different beads containing complementary sequence primers for those 10 adaptors respectively. Hope this helps.
Could you explain in more detail how exactly polony formation takes place? Does the adapter-bound DNA curves as in bridge amplification or is it somehow unbound after the 1st amplification round only to serve as primer in an adjacent adapter?
This is the best explanation of ABI SOLiD that I have found 🔥
it was just awesome
I was struggling to understand the main concept.. and now everything is clear :)
thank you !
Outstanding work. I'm sharing this with my entire molecular biology section.
This is the clearest explanation I've encountered. Thank you!
Awesome video, I have to hold a presentation about the Solid method next week and this video really helped me. ✌✌
Thanks, I'm glad it helped!
ME TOO
Excellent! Thanks, finally I got closer to grasp this...
Very informative lecture thanks a lot🙏 😇 may god bless u with unlimited happiness and peace
Congratulations, very informative video. Your simple and complete way to teach helped me a lot! Thank you very much!
BRAVO!!! Thank you so much, this video is very clear and detailed. I understood everything!
this video was SO helpful and explained very clearly. Thank you
What an amazing presentation!
Nice explanation to every detail. Helps a lot, thanks bro !
Thank you for the wonderful explanation.
Hello, thank you for the great video. I am a non biologist trying to teach myself ngs analysis. I have 2 questions that might have obvious answers however I am not able to understand:
1) why to bother with "a base and color define the next base" when one can use 16 colors instead of 4 ?
2) what is the use of the 3 middle universal bases in the probes? i.e why not just ligate all di-bases at one cycle (instead of the offset 5 times...) and decode the colors ? thanks in advance!
Wow this is amazing. I'm very grateful I've found this video. Thank you!
Thank you! I was having hard time understanding SOLiD-seq but this helped me so much.
great video! please do one about the illumina sequencing.
It`s really nice video for understanding SOLiD. Thank you!
Excellent work!
wonderful explanation... am struggling to understand this concept... now its very clear,Thank you
this is a blessing. Thank you
Simple and effective...Thanks❤
Great work. Thanku so much sir
Great presentation
Thanks,
Can you suggest some review papers
amazing explination . thank you
This is awesome,, i don't know how to thank you 🙏🏻
hello Thanks for the excellent video. Could you please clarify something? The fragments of all unique DNA sequences are flanked by the P1 and P2 primers. Hence when they are binding to the beads, how is it ensured that each bead carries unique DNA sequences. These interactions are with the adaptor sequences right? Hence how is this possible since all DNA fragments carry same adaptor sequences?Thanks.
There are different adaptors for different fragments of DNA. The beads carrying DNA sequences(primers/ oligonucleotides/amplicons)are made by emulsion PCR and one bead is made in such a way that all the primers attached to it carry sequences complementary to any one type of adaptor, among many others. So, suppose there are 10 types of adaptors used to flank the fragmented DNA, there would be 10 different beads containing complementary sequence primers for those 10 adaptors respectively. Hope this helps.
I finally got it! thank you so much
this video was easy to understand, thanks
Excellent presentation Sir... Please do make more videos on molecular techniques in Genetics
Thanks alot, very informative
Wonderful video thank you
very nice explanation! thank you
u should post a thank you video for 125 subscribers!!! keep up the good work!!
Could you explain in more detail how exactly polony formation takes place? Does the adapter-bound DNA curves as in bridge amplification or is it somehow unbound after the 1st amplification round only to serve as primer in an adjacent adapter?
hi. may I request your references for this video? thank you
thanks so much for this video!
great video still a little confused but definitely helped!
PERFECT!
Very helpful! Thank you so much :)
* Permutation = each of several possible ways in which a set or number of things can be ordered or arranged.
Thank you 🙏🏻
Thank you so much
this is very good video thank you
No problem!
Thank you!
Well-put!
really thanks
thank you!
Perfectly explained. Thanks a lot for this video.
you saved me
👍👍
Thank boi
ang pogi ng boses
luff yew
You speak too much fast and the sound is so low