Laser Scanning Techniques and Applications

In this fourth installment of our How to Build a Microscope series, Henry Haeberle will discuss the fundamentals of multiphoton and confocal implementations of laser scanning microscopy.
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0:00 Introduction
1:50 Section 1: Laser Scan Imaging: Fundamentals
6:11 Section 2: Scan Engine
34:25 Section 3: Confocal Specific Scan Engine
38:10 Section 4: Multiphoton Specific Scan Engine Design
45:02 Questions

[@joshuazhang4910]

All around, well explained. Really nice talk!

[@thorlabs]

Glad you liked it!

[@raymondzhao9557]

Very interesting ！

[@thorlabs]

We’re glad you liked it!

[@peterbackx393]

exellent

[@MN-mj9ok]

Thank you for very good explanation. I have one question. What should we consider when using UV as an excitation light in confocal microscopy?

[@thorlabs]

Thank you! We’re glad you found it useful. When using UV as an excitation light in confocal microscopy, make sure that your objective, scan lens, and tube lens have a good enough transmission efficiency and are chromatically corrected for UV.

[@MN-mj9ok]

@@thorlabs Thank you for your answer

[@MN-mj9ok]

Sorry I have a question. What does descanning mean in confocal microscopy?

[@thorlabs]

Thank you for your question! Descanned means that the emission does not have to go through the scanners to reach the detectors.

[@MN-mj9ok]

@@thorlabs Thank you for the quick answer