Bioluminescence of the fungus Panellus stipticus 🍄

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  • Опубликовано: 21 окт 2024

Комментарии • 41

  • @izayaorihara7059
    @izayaorihara7059 2 года назад +12

    I want to do this, too. My to-do list is expanding.

    • @izayaorihara7059
      @izayaorihara7059 Год назад

      @@theo2580
      Saw one fungal seed vendor that sold them for 25€. Currently aquiering a heating system and then off it goes ^^

  • @fancycrafts7774
    @fancycrafts7774 Год назад +2

    Do you think you could spawn these onto coir and they'd fruit, or do they require a hardwood substrate?

    • @AlbertoMelappioni
      @AlbertoMelappioni  Год назад +1

      They generally grow and fruit on decaying hardwood twigs. At least in the wild that's how they do it. 😉

  • @mimmodipalermo
    @mimmodipalermo 3 года назад +1

    Belle immagini, bei suoni ❤

  • @Uiiuoppps
    @Uiiuoppps 6 месяцев назад

    Beautiful video!! I was wondering why does it glow for some part but the rest are not? (for instance, 0:15" shown in the video) I am also growing this fungi in an autoclave bag with grains as substrate, and they are now colonized at a size probably 4cm x 4cm x 4cm, but it still does not glow. Is that because there is not enough oxygen? or what kind of container would be better for it emitting light? as I realized they are easily to be contaminated:))

    • @AlbertoMelappioni
      @AlbertoMelappioni  6 месяцев назад

      Thank you. The mycelium of this species emits light, depending on maturation, oxygen concentration, temperature, etc... but in the specific case of this video, primordia are growing in the brightest points.

    • @Uiiuoppps
      @Uiiuoppps 6 месяцев назад +1

      @@AlbertoMelappioni thank you!! I realized my grain might be too wet(I put them in the water for 24hours before sterilisation), and kept them in the incubator after the colonisation with 27.6 degree. Would that be the problem for it without glow probably? (Coz I saw it can glow even in the small petridish on agar.) Judging by my size of the culture, it should definitely already glow…

    • @devinmartin7626
      @devinmartin7626 6 месяцев назад +1

      Youve got to get your oxy,humidity and temp normalized let er' dry out a bit and keep the temp around 77-88 deg F, and burp them one or twice.

    • @Uiiuoppps
      @Uiiuoppps 6 месяцев назад

      @@devinmartin7626 Thank you so much for the info! btw what does "burp them" mean?

  • @giacomofrattini4456
    @giacomofrattini4456 Год назад

    Ciao. Ho due domande: il fungo qui menzionato e mostrato è di origine europea? Esistono funghi bioluminescenti europei/italiani? Mi piacerebbe provare a realizzare dei sentieri bioluminescenti ma senza funghi alloctoni

    • @AlbertoMelappioni
      @AlbertoMelappioni  Год назад +1

      La specie Panellus stipticus è diffusa sia in europa che in nordamerica, però il ceppo bioluminescente è di origine americana. In europa la stessa specie, pur avendo il materiale genetico per l'apparato enzimatico coinvolto nella luminescenza, per qualche ragione evolutiva non lo esprime. In Italia in particolare trovi Omphalotus olearius che manifesta una discreta bioluminescenza, oppure il micelio di alcuni ceppi di Armillaria mellea. Ti consiglio di provare con Omphalotus olearius. 😉

  • @umbigbry
    @umbigbry Год назад +1

    so i just ordered my own live culture on Panellus Stipticus, and my culture in the syringe doesnt gow like yours does? is this a sign of anything bad?

    • @AlbertoMelappioni
      @AlbertoMelappioni  Год назад +1

      In liquid culture P.stipticus does not emit light. The mycelium manifests luminescence only when it is in contact with the oxygen in the air. Generally when growing in solid substrates such as wood, grain, agar. 😉

    • @umbigbry
      @umbigbry Год назад +1

      @@AlbertoMelappioni ah alright, i just inoculated mine into some grain the other day! hoping for good results

    • @AlbertoMelappioni
      @AlbertoMelappioni  Год назад +1

      @@umbigbry Good luck! 😉

    • @umbigbry
      @umbigbry Год назад +1

      @@AlbertoMelappioni we got some fuzzy!

    • @umbigbry
      @umbigbry Год назад

      ​@@AlbertoMelappioni do you thino i will have an easy time growing mushrooms with it inside a book

  • @umbertopagano7260
    @umbertopagano7260 3 года назад +1

    Innanzitutto complimenti per i tuoi bellissimi video. Volevo chiedere, io ho da un paio di giorni del micelio di Omphalotus nidiformis (Fungo fantasma) che sta crescendo molto velocemente in una piastra Petri. Ora non è luminoso e lo sto tenendo al buio. Per ottenere questo effetto devo metterlo prima sotto la luce o dovrebbe essere così anche se sta sempre al buio?

    • @AlbertoMelappioni
      @AlbertoMelappioni  3 года назад +1

      Grazie! Per quanto riguarda Omphalotus nidiformis, così come per le altre specie bioluminescenti, la reazione chimica che produce la luce avviene sia al buio che alla luce, per intenderci non è un fenomeno tipo fosforescenza che va "caricata". Bisogna però considerare che il sistema enzimatico che catalizza la reazione di bioluminescenza non è sempre attivo durante tutta la crescita del micelio e presenta dei picchi di maggiore attività e quindi luminosità. Generalmente il massimo della luminescenza si osserva nei corpi fruttiferi piuttosto che nel micelio, però non è sempre semplice indurre la fruttificazione di queste specie. ;-)

    • @umbertopagano7260
      @umbertopagano7260 3 года назад

      @@AlbertoMelappioni grazie mille. Una volta che il micelio ha riempito la piastra, cosa consiglia per far nascere il fungo vero e proprio?

    • @AlbertoMelappioni
      @AlbertoMelappioni  3 года назад +1

      @@umbertopagano7260 bisognerebbe tentare l'inoculo di un substrato adatto, purtroppo non ho mai coltivato O.nidiformis, allo stato selvatico in Australia cresce su varie specie dei generi Acacia, Eucalyptus, Platanus, ecc... Io proverei dall'agar a fare uno spawn intermedio con dei grani, per poi inocularci un substrato composto da legno di quei generi di alberi.

    • @umbertopagano7260
      @umbertopagano7260 3 года назад

      @@AlbertoMelappioni grazie mille

  • @rmh7773
    @rmh7773 3 месяца назад

    What type of cereal is used?

    • @AlbertoMelappioni
      @AlbertoMelappioni  3 месяца назад

      I used 50% millet seeds + 50% Phalaris canariensis seeds (canary seed)

  • @Muonium1
    @Muonium1 Год назад

    Hello. We have very similar interests of bioluminescence, radioluminescence, radioactive decay, fluorescence, etc.! This video is very interesting to me but I have a few questions. (I will be making a video soon on how easy and cheap it is to culture brilliantly glowing P. Fusiformis dinoflagellates!) Do you need to periodically add water to this Erlenmeyer flask, and is it simply a piece of Al foil on top? Are the small creases in the metal foil around the neck of the flask sufficient for O2 circulation for the fungi or do you need to take the foil off occasionally to provide more air? Thanks much for your time.

    • @AlbertoMelappioni
      @AlbertoMelappioni  Год назад +1

      The Erlenmeyer flask is closed with a breathable polyester wadding filter which allows the exchange of oxygen and carbon dioxide. In the photo it is not visible, covered by aluminum foil to prevent microbial contamination of the culture during the shots. When the mycelium is incubating I remove the aluminum foil. No need to add water, the substrate is already sufficiently hydrated and consists of millet seeds previously sterilized in an autoclave. 🖖

    • @Muonium1
      @Muonium1 Год назад +1

      @@AlbertoMelappioni interesting grazie mille

    • @AlbertoMelappioni
      @AlbertoMelappioni  Год назад

      @@Muonium1 Your channel is amazing, I've been following for a long time, ever since I found the video about Seaborg in the kids tv show. ☢

    • @Muonium1
      @Muonium1 Год назад +1

      @@AlbertoMelappioni Oh really?? haha, how funny. I had no idea. Thanks very much! More old science documentaries coming soon! I will subscribe to your channel too then, even though I don't speak Italian (...despite the fact that I am Italian, hah). If you ever find you are in America around Western New York for some reason let me know and I can take you on a tour of the world's second most powerful laser driven inertial confinement fusion facility if you like.

    • @xannmax
      @xannmax Год назад

      Hello! Quite curious about the Fusiformis. What sort of precautions should you take for culturing them?

  • @dillonchamberlain
    @dillonchamberlain 9 месяцев назад

    i wonder if it would make a cool liquid culture

    • @AlbertoMelappioni
      @AlbertoMelappioni  9 месяцев назад +1

      Unfortunately, liquid cultures of Panellus stipticus are not luminescent, the mycelium must grow on a solid and be exposed to oxygen.