10x Visium spatial transcriptomics data analysis with STdeconvolve in R

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  • Опубликовано: 8 сен 2024
  • I'm trying out different video styles to teach students about bioinformatics analyses for spatially resolved transcriptomics data. In previous videos, I recorded myself live-coding and narrating through all my thought processes, mistakes, and troubleshooting. Now, I'm trying out sped-up versions.
    So in this video, I speed through coding a bioinformatics analysis in the R programming language to characterize how cell-types are organized in a breast cancer tissue section profiled by the 10X Visium spatial transcriptomics technology (www.10xgenomic...) using our reference-free deconvolution analysis tool STdeconvolve (www.nature.com...)
    Particularly since we have a highschool student interning with us this summer who will be applying STdeconvolve to some new spatial transcriptomics data, I hope this video will be a useful educational resource.
    See our lab blog for the code from this session to try it out for yourself:
    jef.works/blog...
    Some potentially helpful moments:
    - introduction/background: • 10x Visium spatial tra...
    - sped-up coding: • 10x Visium spatial tra...
    - result summary: • 10x Visium spatial tra...
    - lab mascot Remy makes a surprise appearance: • 10x Visium spatial tra...
    Music by Stockaudios (pixabay.com/us...)

Комментарии • 5

  • @juliangrandvallet5359
    @juliangrandvallet5359 Год назад

    Thank you, Dr., Please keep doing these videos! they are very helpful

  • @taobohu1402
    @taobohu1402 Год назад

    Hi Prof. Fan, thank you so much, very nice tools! And very cute dog :)

  • @izabela8725
    @izabela8725 8 месяцев назад

    great videos, very helpful! Will you by any chance also analyse Xenium spatial transcriptomics data from 10X?

  • @0jaxay0
    @0jaxay0 5 месяцев назад

    nice

  • @relaxenjoy9817
    @relaxenjoy9817 9 месяцев назад

    Thank you
    Does it mean, that if one is using SpaceRanger v2 to process the sequencing Visium data, using STdeconvolve in R is unnesecary afterwards?
    best