10x Visium spatial transcriptomics data analysis with STdeconvolve in R
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- Опубликовано: 8 сен 2024
- I'm trying out different video styles to teach students about bioinformatics analyses for spatially resolved transcriptomics data. In previous videos, I recorded myself live-coding and narrating through all my thought processes, mistakes, and troubleshooting. Now, I'm trying out sped-up versions.
So in this video, I speed through coding a bioinformatics analysis in the R programming language to characterize how cell-types are organized in a breast cancer tissue section profiled by the 10X Visium spatial transcriptomics technology (www.10xgenomic...) using our reference-free deconvolution analysis tool STdeconvolve (www.nature.com...)
Particularly since we have a highschool student interning with us this summer who will be applying STdeconvolve to some new spatial transcriptomics data, I hope this video will be a useful educational resource.
See our lab blog for the code from this session to try it out for yourself:
jef.works/blog...
Some potentially helpful moments:
- introduction/background: • 10x Visium spatial tra...
- sped-up coding: • 10x Visium spatial tra...
- result summary: • 10x Visium spatial tra...
- lab mascot Remy makes a surprise appearance: • 10x Visium spatial tra...
Music by Stockaudios (pixabay.com/us...)
Thank you, Dr., Please keep doing these videos! they are very helpful
Hi Prof. Fan, thank you so much, very nice tools! And very cute dog :)
great videos, very helpful! Will you by any chance also analyse Xenium spatial transcriptomics data from 10X?
nice
Thank you
Does it mean, that if one is using SpaceRanger v2 to process the sequencing Visium data, using STdeconvolve in R is unnesecary afterwards?
best