I would have love to see example. 1 sample with proper thickness Vs 1 sample with cover too thick or too shallow. I very appreciate your nice voice, but it's always nice to get visual support as well. Ps Today I've just buy my first ever microscope material: cover glass, slides, 4x, 10x, 40x and 40x FLUOR. I'm working on a project: 3D printing my own microscope!!!!
I have heard you say before that 100x objective is used w/o coverglass. Strange. My 100x objective says 0.17. I would never use no cover glass with oil.. Specimens would start floating etc..
old comment though i believe he was talking about baked specimen slides without a cover slip. He talks about it in a different video, definitely a lapse in detail here though to not specify
Nice ,clean,full info over one misterium for students and many laboratory people. Are so many funny laboratory stories about students and old lab researchers what focus over cover glass and not direct over specimen on slide. :)))))) Good work ! ;)
can you please post a 40x SPlan Olympus microscope video that shows, with the right coverglass and so on, that it is well focused? 60x would be great too....
@@MicrobehunterMicroscopy interesting.... And what type of objectives do you have on your CH 40? DPlan? In short, whatever type they are, I think that many Amateure Microscope users have trouble focusing at 40x. I think that it is an "art" to improve focus at this level of magnification (and up), yes? It seems to involve slide, coverslide and specimen thickness, how far (or close) the condenser is to the specimen, how clean the respective objective and condenser lenses are, the degree to which the condenser diaphragm is open/closed and the kohller diaphragm (if there is one), and is there more to it? It is not a rhetorical question, meaning that I still have crappy focus at 40x )))
My microscope has a screw on its mechanical stage that set the maximum high that the stage can reach. How does it have to be set that screw? Because if it's too low, then you can't focus well with the 4X objective and if it's too high then there is a risk, that has already happened, to make the objectives touch the cover glass, especially the 40X and 100X ones. Thank you very much! 🙏 (For now I have set that screw in a way that the specemen is out of focus a little bit once the stage is all up near the 4X objective, and then I have to low the stage a bit to put the specimen in focus again, is that right?)
Theoretically it is possible if you use the correct solvent. But i would not do that. Some solvents are toxic and chances are very high that you break slide and/or cover glass. Slides and cover glasses do not cost much anyway. A prepared slide is much more expensive than a blank slide.
@@MicrobehunterMicroscopy actually that is not the case with me prepared slides are more cheaper the blank slides but anyhow I am still saving up I will use top light microscopy until I save up again for slides (prepared and blank) THANK YOU
Crazy how you answer all the questions :D good job!
I would have love to see example. 1 sample with proper thickness Vs 1 sample with cover too thick or too shallow.
I very appreciate your nice voice, but it's always nice to get visual support as well.
Ps Today I've just buy my first ever microscope material: cover glass, slides, 4x, 10x, 40x and 40x FLUOR. I'm working on a project: 3D printing my own microscope!!!!
Have you talked about depression concave slides ?
When are they needed or used?
I really enjoy your videos.
I use these slides here: ruclips.net/video/j11_Wi5IEpk/видео.html
Clear and informative. Great video.
I have heard you say before that 100x objective is used w/o coverglass. Strange. My 100x objective says 0.17. I would never use no cover glass with oil.. Specimens would start floating etc..
old comment though i believe he was talking about baked specimen slides without a cover slip. He talks about it in a different video, definitely a lapse in detail here though to not specify
I'll be sorting out my bulk buy slides and cover glasses with a micrometer, thank you.
do you reuse cover glass ? When I observe pond life I tend to wash everything (slide & cover slide) and reuse them. I that recommended? thank you!
Hi microbehunter I like you videos
Nice ,clean,full info over one misterium for students and many laboratory people. Are so many funny laboratory stories about students and old lab researchers what focus over cover glass and not direct over specimen on slide. :)))))) Good work ! ;)
can you please post a 40x SPlan Olympus microscope video that shows, with the right coverglass and so on, that it is well focused? 60x would be great too....
Unfortunately I don't have an SPlan objective..... But I can make a video on how different coverglass thicknesses influence resolution.
@@MicrobehunterMicroscopy interesting.... And what type of objectives do you have on your CH 40? DPlan? In short, whatever type they are, I think that many Amateure Microscope users have trouble focusing at 40x. I think that it is an "art" to improve focus at this level of magnification (and up), yes? It seems to involve slide, coverslide and specimen thickness, how far (or close) the condenser is to the specimen, how clean the respective objective and condenser lenses are, the degree to which the condenser diaphragm is open/closed and the kohller diaphragm (if there is one), and is there more to it? It is not a rhetorical question, meaning that I still have crappy focus at 40x )))
My microscope has a screw on its mechanical stage that set the maximum high that the stage can reach. How does it have to be set that screw? Because if it's too low, then you can't focus well with the 4X objective and if it's too high then there is a risk, that has already happened, to make the objectives touch the cover glass, especially the 40X and 100X ones. Thank you very much! 🙏 (For now I have set that screw in a way that the specemen is out of focus a little bit once the stage is all up near the 4X objective, and then I have to low the stage a bit to put the specimen in focus again, is that right?)
I have a Zeiss winkel 100x /-
No cover glass. ???
Oil immersion objectives do not need a cover glass. Therefore a cover glass thicknes is often not indicated on the objective.
Hi i wanted to ask a question can i remove the specimen in a prepared slide and use the slide and the cover glass
THANK
YOU
Theoretically it is possible if you use the correct solvent. But i would not do that. Some solvents are toxic and chances are very high that you break slide and/or cover glass. Slides and cover glasses do not cost much anyway. A prepared slide is much more expensive than a blank slide.
@@MicrobehunterMicroscopy actually that is not the case with me prepared slides are more cheaper the blank slides but anyhow I am still saving up I will use top light microscopy until I save up again for slides (prepared and blank)
THANK YOU
👍👍👍
Difficulty breathing
for you amateurs and microscopy hobbiists the thickness of cover glass is really not important