How to dilute sample for UV Visible measurement

[@chemcolony]

Just wow🎉❤

[@duhasaadi350]

thank you for this note
in the case of using thin film can you explain how the UV
measurement will be achieved

[@Aminulchem]

Thanks. Is that liquid state?

[@susantamohapatra2757]

How dilution factor used in the uv visible spectroscopy?

[@Aminulchem]

@@susantamohapatra2757 If the absorbance of your solution is above 2 in the UV-visible measurement, then you need to dilute your solution to make absorbance below 2. Please watch other videos and subscribe to this channel.

[@SanoberGul_14]

Can you share your email id?

[@sumanpatra7380]

Sir I am doing green synthesis of CuO Nanoparticles. Can you please explain at which stage I take the sample for OD? synthesis mixture before centrifugation or calcination powder? Please suggest me sir❤

[@Aminulchem]

Thanks. I think it is better to consult with your supervisor. He can guide you well.

[@SanoberGul_14]

I have a question sir,
Please can you guide how to do dilution for UV visible spectrophotometer analysis, like after experiment i have samples and one balnk pollutnt solution, now do i need to dilute all the sample including control with same Dilution factor? Please guide me

[@Aminulchem]

Thanks. It is better to use the same dilution factor if absorbance goes above or near 2.

[@SanoberGul_14]

@Aminulchem ok thank you sir

[@Aminulchem]

@@SanoberGul_14 Welcome. Please also watch other videos and subscribe to this channel.

[@Aminulchem]

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