Making and Testing The ANTI-SPICY Molecule (Capsazepine)

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  • Опубликовано: 25 дек 2024

Комментарии • 401

  • @Chemiolis
    @Chemiolis  Год назад +20

    Subscribe to Curiosity Stream and start exploring the world around you! bit.ly/CuriosityStreamChemiolis1223 or use the code: Chemiolis

    • @cezarcatalin1406
      @cezarcatalin1406 Год назад

      There is a much simpler chemical that selectively blocks TRPV1.
      en.wikipedia.org/wiki/AMG-9810
      You could make this really really easily.
      It also probably tastes better since it has a more neutral pH and it doesn’t trigger noxious chemical receptors.

    • @highlander723
      @highlander723 Год назад

      tasting your own chemical product...... That's either really stupid or fucking savage!

    • @Zane.Wellnitz
      @Zane.Wellnitz Год назад

      Getting rid of the burn and the taste is no help. The spice is still going to do the damage, also you are still going to feel the burn on its way out😱💥💩

    • @YoKnow
      @YoKnow 11 месяцев назад

      I believe I am the man your looking to prove this. Perhaps I can prove you right!

  • @skeptical_thinkers
    @skeptical_thinkers Год назад +456

    I was wondering when someone would finally get around to this synthesis! I actually managed to get a hold of some Capsazepine a couple years ago and my experience was similar. For me it reduced the spicyness, but only for about a half an hour, and didn't reduce it any more than perhaps drinking a glass of milk.

    • @JaredBrewerAerospace
      @JaredBrewerAerospace Год назад

      Been breeding Ghost Peppers for 14 years, milk and bread are placebo. The real trick is honey.

    • @nunyabisnass1141
      @nunyabisnass1141 Год назад +15

      Is it possible that it's like capsaicin and needs to be in a salt form to be effective? Or was it already prepared injestion? (When i say salt form, something that will disolve in water as pire capscaicin doesn't have a strong effect until it dissolves slowly).

    • @iWhacko
      @iWhacko 11 месяцев назад +3

      @@nunyabisnass1141 I was told, alcohol latches onto the capsaicin even better. Taking a shot of tequila and swirling it around in your mouth after eating something spicy would flush it out. it WILL HURT a lot more than the spice, but it's for a shorter period, because the capsaicing is flushed away and doesn't dislodges from the receptors

    • @nunyabisnass1141
      @nunyabisnass1141 11 месяцев назад +2

      @@iWhacko i have no idea, I know that it's more soluble in alcohol, but that's as far as my knowledge goes.

    • @welporajackwelp4899
      @welporajackwelp4899 10 месяцев назад

      Did you take it before or after you ate the spicy food?

  • @andrewamann2821
    @andrewamann2821 11 месяцев назад +61

    So... It prevents the burn going in... But that's only half the battle, sir.

    • @ryanjohnson4565
      @ryanjohnson4565 3 месяца назад +2

      Please elaborate in detail.

    • @GatorShades171
      @GatorShades171 3 месяца назад

      ​@@ryanjohnson4565🍑🔥🧯

    • @TheHuntermj
      @TheHuntermj 3 месяца назад

      I assume it would also affect nerves elsewhere if you swallowed it...

    • @andrewamann2821
      @andrewamann2821 3 месяца назад +1

      @@TheHuntermj I suppose that depends on the dosage, and how well it survives stomach acid and enzymes... The mouth is a far less harsh environment, chemically, than the rest of the digestive tract.

    • @scottsammons7747
      @scottsammons7747 2 месяца назад

      Just need to also make suppositories...
      Actually, suppositories may be the superior product. Many times the pleasure in consumption was overshadowed by fear of the outcome.

  • @hammerth1421
    @hammerth1421 Год назад +81

    That's an impressive multi-step synthesis for "amateur" chemistry!
    It also serves as a neat demonstration of the "general synthesis loop" of organic chemistry: dissolve in solvent -> reaction -> workup -> distill off the solvent -> repeat

    • @chessgaming9942
      @chessgaming9942 11 месяцев назад +9

      Well, amateur doesn’t really denote a lack of skill, but rather an independent chemist who works outside of a laboratory/company. It is, however, a very impressive reaction route.

    • @asialsky
      @asialsky 11 месяцев назад +8

      ​@@chessgaming9942I think you've passed "amateur" in chemistry when you can look at a molecular chain and go "yeah, that'll explode if exposed to oxygen."

    • @scottsammons7747
      @scottsammons7747 2 месяца назад

      ​@@chessgaming9942Amateur= lover of the subject, ie motivated by love rather than money.

  • @thomasrussell4674
    @thomasrussell4674 Год назад +283

    Theres another anti spicy compound.
    Your capsazepine just blocks heat, but there's another one that functionally reverses the action of the trpv1 receptor, it's called
    "Iodoresiniferatoxin".
    If you consume it, it makes you feel cold.
    Im guessing here i could be wrong but i believe menthol is different, and works on a cold sensing receptor, but iodoresiniferatoxin actually latches onto a generally heat sensing receptor with an action functionally the opposite of capsaicin.
    I hope you enjoy reading about it!
    You might be able to tell me, is iodoresiniferatoxin just copying menthol and they all just work on the same receptor, *OR* if it really is different and iodoresiniferatoxin is unique, pretty much in the same league as capsazepine with your one in this vid blocking the receptor, and iodoresiniferatoxin acting as an antagonist at the same receptor.
    Great video btw, thanks!

    • @pente12
      @pente12 Год назад +59

      IRTX and capsazepine are both antagonists of TRPV1 which means they block activation of the receptor. You might be talking about inverse agonism, but I don’t think IRTX is an inverse agonist of TRPV1 and I also don’t think inverse agonism if TRPV1 would give a cooling effect. Like you said, menthol acts via a different receptor to produce to cooling sensation.
      What makes IRTX so interesting is that the non-halogenated RTX is an extremely potent agonist of TRPV1 (1000 times stronger than capsaicin), and adding that single iodine completely eliminates the molecule’s ability to activate the receptor

    • @thomasrussell4674
      @thomasrussell4674 Год назад +23

      @@pente12 yeah it is interesting isn't it.
      Just like in the most crude visual sense, estrogen and testosterone are vaguely similar looking molecules.
      And you can take a very potent traditional opioid, oxymorphone, and just at a single allyl group to the nitrogen and suddenly you have a potent antagonist naloxone.

    • @Sniperboy5551
      @Sniperboy5551 Год назад +21

      @thomasrussell4674 Yup, pharmacology is wicked cool. There are also opioid agonists and antagonists that covalently bind to opioid receptors, so they basically work for days until the receptors themselves are broken down. They’ve done a lot of pharmacological research into different opioidergic compounds, so that entire class of drugs is very intriguing to learn about.

    • @thomasrussell4674
      @thomasrussell4674 Год назад

      @@Sniperboy5551 yes you're exactly right and the only covalent bonding example I know is oxymorphone itself.
      I'd like to know if fentanyl or etorphine is capable of this additional form of receptor bonding. It's funny the way some old fashioned super strong opioids like levorphanol and oxycodone were actually quite long acting, while some modern high potency synthetics are ultra short acting.
      Interestingly the covalent bonding rapidly accelerates the formation of tolerance, because nearly all opioids cause beta arrestin recruitment and lead to receptor internalisation, but normally this requires repeated and frequent dosing to trigger the cell/the body (which seeks homeostasis and reacts to continual over activation of an opioid receptor as unusual and something to be stopped) to effectively put the overstimulated receptor out of commission. Actually homeostasis works in at least 3 ways to counteract ongoing opioid intake, 1. Plasma antibodies to bind and inactivate an exogenous opioid before it gets to a receptor, 2. Internalisation 3. Eventual upregulation of substance P to maintain pain perception and gut motility and 4. Something to do with expectation, I can't remember the exact mechanism but there's a weird counterintuitive nocebo effect which can be blocked with proglumide, but only if the person was expecting a drug dose, basically the body tries to prepare itself if the person is in a state of anticipation and it secretes a type of neurokinin, but proglumide can block it.
      Only 2 opioids I know don't cause beta arrestin recruitment. 1 is a strange, non-nitrogenous relative of salvinorin but it's not a mu agonist, I think it might be delta. 2 was a big pharma designer opioid called oliceridine which was designed not to, supposedly to be less addictive....But it didn't make it through multistage clinical trials.
      There's another fascinating old drug

    • @thomasrussell4674
      @thomasrussell4674 Год назад +5

      Sorry it was meant to say levorphanol and oxymorphone , but it all ends up the same because of cyp2d6

  • @lrvfb
    @lrvfb Год назад +285

    Man, you didnt really just eat a chemical in a long multi-step synthesis without even doing a column after the last step?!?😂😂

    • @diacoal2433
      @diacoal2433 Год назад +108

      He isn't scared of the byproducts, he IS the byproduct.

    • @bromisovalum8417
      @bromisovalum8417 Год назад +15

      It's a very small amount, but still... it's always a risk.

    • @ZeroPlayerGame
      @ZeroPlayerGame Год назад +71

      "it's more convenient to purify it later"
      purification later: wash it with some water and into the mouth it go

    • @guygordon2780
      @guygordon2780 Год назад +19

      The problem with home chemistry labs. No money for test equipment. Some things just take a University.

    • @lokiaverro4196
      @lokiaverro4196 Год назад

      @@guygordon2780 apparently not this thing, though, since he got it done

  • @liammccreary2941
    @liammccreary2941 Год назад +24

    I run Wittig reactions all the time and you don’t realize how much work you’ve just saved me! I swear I’ve looked everywhere to find validated methods for removal of PPh3O and I’ve never heard of this paper! The different salts for different solvent systems chart is so helpful!

  • @nunyabisnass1141
    @nunyabisnass1141 Год назад +11

    I found a little on this chemical. While capsaicin stimulates the TRPV1 receptor responsible for sensing heat and pain, capsazepine blocks and hinders the function of this receptor. While it sounds all well and good that it can limit the pain from spicy foods and potentially offer some burn relief, strictly uncontrolled doses can be too effective by tricking the body into not realising its warm and cause your body temp to rise, possibly to dangerous levels.
    So dont just go on thinking you found a cheat code foe next uears chili cookoff, or stock your first aid kit with it.

    • @jbdawinna9910
      @jbdawinna9910 2 месяца назад

      Yo, next, hot weight loss supplement!? Causing you to burn fat and overheat and hopefully not die???

    • @nunyabisnass1141
      @nunyabisnass1141 2 месяца назад

      @@jbdawinna9910 it could def have some useful medical applications assuming there's no other adverse reactions. But yeah, a controlled dose could in theory stimulate metabolism, though you might feel cold while it does so.

  • @Ismft
    @Ismft 11 месяцев назад +25

    "And here we add the brother to a literal chemical warfare weapon, but don't worry this one smells like meat. Now its time to taste..."

  • @1leon000
    @1leon000 5 месяцев назад +13

    0:12 Now if that wasn't funny enough, here are two words that'll make this even funnier: He's Dutch

  • @pauljames5826
    @pauljames5826 11 месяцев назад +10

    I was surprised at an Indian restaurant where they served no alcohol but did have mango nectar as an aperitif/beverage. While eating some of the mixed crisps only one item in the mix was SPICY - I grabbed my mango nectar and took a drink - to my surprise, the heat was stopped. I’m thinking that papain might have been the actor in this pleasant discovery(?)

  • @tim-tim-timmy6571
    @tim-tim-timmy6571 Год назад +76

    I can't remember the name but at university I got to try a compound (tasting awful) that inhibits the ability to taste sweetness. After trying it, I got as many sweets as possible. Cookies: dense concrete. Honey: Salty paste.
    I tried to look it up and I can't find the name of the molecule. If you're up to another experiment, I would love to see you synthetize it and then get to try all sweets you got at hand. That was a funny experiment!

    • @MalleusSemperVictor
      @MalleusSemperVictor Год назад +24

      Sounds like some kind of gymnemic acid. It is derived from a plant known in Hindi as _gurmar_ which means sugar destroyer.

    • @jamesyoungquist6923
      @jamesyoungquist6923 Год назад

      Try chewing up ibuprofen pills. You'll get a similar effect

    • @sashaleggett1994
      @sashaleggett1994 Год назад +28

      @@trippyvortexit's the opposite. people use it to lose weight, especially those with diabetes, as it makes it less desirable to eat the sweets.

    • @chrism4008
      @chrism4008 Год назад +2

      beetus

    • @Poodleinacan
      @Poodleinacan 11 месяцев назад +2

      ​@@trippyvortexyou either have pica or misread the comment 😅

  • @chemdelic
    @chemdelic Год назад +14

    I can’t begin to imagine the work you put in on this. Great job brother!

    • @searsbear7965
      @searsbear7965 10 месяцев назад

      Hey it’s the other chemistry guy

  • @erokfussell
    @erokfussell Год назад +5

    Pack your column by running several column lengths of solvent prior to adding sand. Tighter packed columns give better separation as well!

  • @nathanieljames7462
    @nathanieljames7462 Год назад +22

    Non-chemists when he says he washed it with water: The f*ck you did.

  • @apokiedd
    @apokiedd Год назад +12

    Once I have eaten ginger caramel and then spicy chocolate. It was definitely less spicier than this chocolate alone, and I have tested this multiple times. Now I am wandering that maybe some molecule in ginger candy had similar effect.

    • @cafeinoz
      @cafeinoz Год назад +19

      Ginger contains gingerol, which is chemical that also activates trpv1 like how capsaicin does. I assume it would either activate the receptor weaker than capsaicin and compete with the capsaicin trying to bind with the receptor, or it might desensitise the receptor making the action of capsaicin weaker.

  • @thetaintpainter5443
    @thetaintpainter5443 Год назад +24

    How'd the Pd/C filtration go? I had to do that a lot after hydrogenations. one time I got absent minded and keep the vacuum running well past the absence of solvent, forgetting about the pyrophoric properties. Filter paper burst into flames lmao

    • @RaunienTheFirst
      @RaunienTheFirst 3 месяца назад

      Ah, we've all done stupid shit in a lab lol. I once rinsed out a Buchner funnel with water, forgetting that it had been used to filter out sodium hydride. The pyrotechnic display was not appreciated.

  • @robelbelay4065
    @robelbelay4065 11 месяцев назад

    Awesome step but step verbal explanation of each reaction and their intermediates, trying to get into curb and this really helps

  • @michaelschroeder7571
    @michaelschroeder7571 11 месяцев назад +7

    After 16 million chemical steps and washings, and distillations etc etc etc, you are one ballzy dude to actually consume this end product! No way I would do this.

  • @DaveMuller
    @DaveMuller 5 месяцев назад +1

    My favourite part was watching 25 minutes of chemistry for the final result...
    "It tastes a bit foul"
    Well worth the watch, and well worth how ever many weeks of work you went through to make a foul chemical.

  • @timothydestiny3865
    @timothydestiny3865 4 месяца назад

    Thank you very much! You are now my favorite chemistry RUclipsr. I know it took you a week to make this.

  • @lobomirtopalov6599
    @lobomirtopalov6599 11 месяцев назад +1

    11:48 poor stirbar holding on for dear life

  • @mduvigneaud
    @mduvigneaud Год назад +6

    I love this video! :D Personally I like the pain from eating spicy food. Y'know, the huge endorphin rush from the pain. lol

  • @lasagner9567
    @lasagner9567 11 месяцев назад +3

    somebody let this man know you can just drink milk

  • @GuentherVanRaven
    @GuentherVanRaven Год назад +19

    Guys, I need advice.
    I‘m in my 3rd year of Bachelor in Chemistry. I‘m currently struggling with my motivation, I‘m exhausted from all the stress, the hours of lab work, the days of writing reports, the hours of commuting etc. It feels like it caught all up to me, the lack of a balanced life in the past years. As an introvert it‘s really tiring. Grades were always good, did not struggle too much because of the grades, it‘s just the amount of work. And right now I don‘t have any motivation left, I honestly think chemistry sucks. I remember first year, full of motivation and then it slowly decreased.
    Do you guys have any advice or similar stories?

    • @JonMacCaffrey
      @JonMacCaffrey Год назад

      "We saw NileRed have multiple failed attempts at the synthesis of benzaldehyde... likely due to some small mistakes that ended up ruining the whole experiment; which is quite typical for chemistry, and there is some art in finding and fixing them which often requires advanced knowledge of chemistry, ACQUIRED THROUGH MANY YEARS OF SUFFERING" - Chemiolis
      Focus on how rewarding it will be to be able to do things the majority of humans can't even comprehend! And when you apply for a job, you'll get way more money if you search for "material manufacturing" instead of "chemistry or lab work" Like 100k+ manufacturing coatings for anything from lubricating gears to coating spaceships for NASA or SpaceX

    • @markedis5902
      @markedis5902 Год назад +16

      Talk to the university’s welfare department. It’s what it’s there for and they may be able to help. They are used to burnout and should have procedures in place. Don’t be put off and nag them until you get a result.

    • @BoredCoat
      @BoredCoat Год назад +2

      Just at the end of my 1st semester, 2nd year of studying chemistry of materials and chemical engineering. Am not that far as you, obviously, but have little to no motivation to do anything. I think the only motivation I have is that I like learning chemistry (while also hating it, insane, right? xd). I just look at some people and want to do the work they do and to do that I need to finish at least the Bc. But I would literally trade the ability to make PCHEM disappear instead of world peace, ngl.

    • @Renvoxan
      @Renvoxan Год назад +4

      Life is a marathon, it will be OK bro 🫡

    • @margodphd
      @margodphd Год назад +6

      Everything worth doing is difficult. The coolest shit has tough moments, otherwise - everyone would be doing it.
      If you are continuously over- burdened, burnout is imminent. Think how could you lessen the load - can you share the work differently? Move somewhere closer or change hours you have to be physically present at the lab so the commute is taking less time? Could you talk with department to swap some hours?
      Perhaps the shorter days and lessening amount of daylight is affecting your mood. Think of how you can incorporate daylight exposure - a short,brisk walk a day helped my mood and energy levels tremendously. You can always use this time to listen to lectures or audio notes, but I think it's more valuable to unwind.
      Have you thought about utilising some AI tools to lessen the workload? It's not a sin to use AI to redact lab notes, or rephrase papers or notes but I've heard it can cut out some of the tedious aspects of work.
      Remember, the uni department is there for you - contact them if you are struggling. Asking for help is an important skill.
      Remember that the boring parts are a part of a test and as all troubles - are temporary.
      Don't make same mistake I did and run yourself so far into ground with pointless perfectionism that there's no way out but to give up.

  • @dabyd64
    @dabyd64 Год назад +11

    This could very well be a commercial product!
    I'm sure it would have it's place, i.e. I love spicy food, but after some genetic-related surgeries, anything more than a pinch of pepper will destroy my guts and leave the exit hole like The Eye of Sauron.

    • @kaleoscreations
      @kaleoscreations Год назад +20

      I think this does the opposite: makes you not taste the spicyness, but it’d still destroy your guts and third eye

    • @Tunkkis
      @Tunkkis Год назад +16

      ​@@kaleoscreations The worst of both worlds.

    • @winkus8586
      @winkus8586 Год назад +3

      Although not as serious as yours. My gut also can't tolerate too much capsaicin.
      My tongue/mouth are enjoying capsaicin. The heavier the dose the better. But i will have to go to the toilet multiple times when i got too much of it

  • @isaacm1929
    @isaacm1929 Год назад +10

    Home chemistry labs, one of the only places where you make human drug testing, while also making a counter to police warfare.

  • @nathanieljames7462
    @nathanieljames7462 Год назад +4

    Eating my favourite combination of spicy compounds as I watch this.
    Just feels proper.

  • @colettechausser7879
    @colettechausser7879 3 месяца назад

    There's definitely not greater problems to find solutions for than this. Best and brightest.

  • @MakotoIchinose
    @MakotoIchinose Год назад +1

    As an Indonesian who can't stand spicy foods, this would be my helper whenever I had to eat in public.
    Indonesians are notorious for obsession with spicy foods, but not me. At least sour drinks help neutralize the pain a bit everytime.

  • @Petemate69
    @Petemate69 Год назад +1

    Few comments: For the LiAL4 quench, a fieser style work up is much safter and uses readily available chemicals.
    "In the Fieser work-up, following reduction with n grams of LAH, careful successive dropwise
    addition of n mL of water, n mL of 15% NaOH solution, and 3n mL of water provides a
    granular inorganic precipitate that is easy to rinse and filter. For moisture-sensitive
    substrates, ethyl acetate can be added to consume any excess LAH and the reduction
    product, ethanol, is unlikely to interfere with product isolation"
    For the column i'd apply air pressure above your column if possible, it will help pack the column. Move 1-2 column volumes through and then load the column and it will be nice and packed.

  • @TestECull
    @TestECull Год назад +14

    Love the synthesis, but uhh...realtalk, if you want to resolve the burning when you eat something spicy, just eat the spicy thing and wash it down with a sip of whole milk. the milkfats almost immediately cancel the 'spicy'. The more you do that, the higher your tolerance to the heat gets, until you no longer need the milk at all.

    • @STEAMerBear
      @STEAMerBear Год назад +3

      This was my own youthful experience. Now, 4 decades later, about 6-18 hours after my spicy meal my family members stand in the hall outside my locked bathroom door asking why I keep yelling, “Come on ice cream!”

    • @TestECull
      @TestECull Год назад +1

      @@STEAMerBear I had that for a few months, but in time, both ends grew accustomed to spice and now it isn't even a thing. Just flavor really.

    • @TestECull
      @TestECull Год назад

      @@thricegreat7175 Very much the truth. Try it for yourself. throw some cayenne pepper powder on your tongue then take a swig of whole milk. Burn disappears. Repeat but with water, burn just gets worse.

    • @TravisTerrell
      @TravisTerrell Год назад +4

      Yeah, I'm a HEAVY milk drinker and can 100% confirm this is not true. If does help, like other fats, but nah, it's a far, far cry from "cancelling the spicy."

    • @TestECull
      @TestECull Год назад

      @@TravisTerrell I'm gonna listen to my own tastebuds over a rando on a youtube comment section 🤷 and my tastebuds confirm it to be right on the money.

  • @AndAbel-AOD
    @AndAbel-AOD 6 месяцев назад

    ClO2 seems to completely neutralize capsaicin. I was getting to a point where I could no longer handle going up on the reapers added to tacos, until I accidentally discovered ClO2's neutralizing effect. It neutralizes the heat in the mouth but most importantly(for me) the stomach and beyond.

  • @whispy2368
    @whispy2368 11 месяцев назад +1

    Man this synthesis is long and tedious, but somehow its cool to experience it, that freaking decantation flask was the most common aside from ethyl acetate, both were probably the most used throught the video, congrats btw :D

  • @TealRubyy
    @TealRubyy 3 месяца назад

    My guy is crazy for tasting that end product without cleaning it up more. I could never

  • @Plancksized
    @Plancksized 6 месяцев назад +1

    In the reduction with pd/c why does the carbonyl not reduce as well? I assumed that pd/c reduction was powerful enough to do so

    • @zhen_leo35
      @zhen_leo35 2 дня назад +1

      Turns out, regular hydrogenation with Pd/C is not effective for carbonyl compounds

  • @Nota_Chemist
    @Nota_Chemist Год назад +3

    Watching this as I drink spicy coffee, another gorgeous viewing experience!
    Gotta love the probabilities of seeing this as I upload a video on capsaicin extraction 😅

  • @lamborn3D
    @lamborn3D 11 месяцев назад +5

    Your channel page says that you're based in the Netherlands, so I assume English is not your native language. However, your grammar is much better than the grammar of most native speakers post-commentary videos I have seen. It's not a thing I like to complain about, but it is such a breath of fresh air not to hear people constantly going back and forth between past tense and present tense in the same sentence. So thank you for that, and well done.

  • @alinisyif6000
    @alinisyif6000 3 месяца назад

    Seeing octopuslady’s platform get some love has made my day

  • @durshurrikun150
    @durshurrikun150 Месяц назад

    When I did a Wittig reaction I was able to eliminate phosphine oxyde by column, it depends on the polarity.
    Although my products were less polar than the oxyde

  • @TheReaverOfDarkness
    @TheReaverOfDarkness 4 месяца назад

    There is an alternative anti-spice method which is more easily done at home: just mix in a large amount of corn starch and oil. There is a brand of ramen which I absolutely love except that it is excessively spicy by my standards. I use this method to be able to enjoy it!
    Note: The food will still be spicy, but it will be like half to a quarter as spicy, or less. If your food is still too spicy, consider adding a more generous amount of corn starch and oil.

  • @CendronesFr
    @CendronesFr Год назад +1

    Nice work here, I've got a question though, at around 18:20 you get to layers after adding NaHCO3 solution to the reaction mixture. The solvant is supposed to be Acetonitrile, which is miscible with water. Did you already add DCM to the mixture ?

    • @geordifrere
      @geordifrere Год назад +2

      Acetonitrile becomes immiscible with aqueous solutions of very high ionic strength (brine, sat. NaHCO3). DCM would have formed a layer on the bottom.

    • @CendronesFr
      @CendronesFr Год назад

      Yeah, didn't think about positions of the layers. However I didn't know about the ACN, I've been using for years as a LC solvant. Thanks !

  • @llihpmeHnevetS
    @llihpmeHnevetS Год назад +1

    What chemical is in the miracle berry? That might get you what you’re looking for.

  • @one-iron
    @one-iron 11 месяцев назад +1

    I actually really enjoy columning, but doing DCM/MeOH columns is annoying. For the LAH reduction it wouldn't have been necessary, would it?

  • @LadyLexyStarwatcher
    @LadyLexyStarwatcher 11 месяцев назад +2

    Huh, cool! I have often wondered if there was a compound that blocks Capsaicin receptors. Neat! Yeah, wet chem, and organic at that, is just a slog. That's why I went computational. XD Good video.

  • @robertkeyes258
    @robertkeyes258 Год назад +1

    Ah yes this should be any the travel kit of anyone going to Thailand.

  • @mikaljan
    @mikaljan Год назад +2

    your chemistry knowledge clearly surpass NileRed

  • @TheLobotomist-t1
    @TheLobotomist-t1 5 месяцев назад

    One way to completely remove the ability to taste spicy: drink soda with 1.5ml GBL mixed in, and you won't be able to notice any spice. it will strip the enamel off your teeth tho, so don't do it too much! For those unaware, GBL converts into GHB in the stomach and gives the same high

  • @theredstormer8078
    @theredstormer8078 3 месяца назад

    12:40 what a nice purple color. After seeing how this stuff is made, I would be VERY hesitant to put it anywhere near my mouth. I'm no chemist, I just heard a lot of things that sound very toxic and dangerous. I'm sure the final product is relatively safe in small amounts.

  • @DarkstarPyro
    @DarkstarPyro Год назад +1

    It might be quicker to just learn to like spicy foods 😂

  • @petevenuti7355
    @petevenuti7355 Год назад +2

    Ah. But how did you make the thiophosgene?
    (I was the guy picking you brain about making a isothiocyanate about a year or so ago, looks like you got that figured out nicely!)

    • @ashe1.070
      @ashe1.070 Год назад +2

      It looks like he bought it from Sigma Aldrich. The bottle looks like a Sigma SureSeal bottle.

  • @geordifrere
    @geordifrere Год назад +1

    Did you notice any quinone products from oxidation of the catecholamine in air? I see a lot of dark-coloured reaction mixtures which signal to me that oxidation may have occurred. Especially doing these reactions in air at elevated temperatures, I would worry that the final product is at best contaminated with the potentially toxic quinone and at worst fully converted.

    • @nickburgers5177
      @nickburgers5177 Год назад

      The man didn't notice anything. Just closed his eyes and pushed it through, and i highly doubt it he actually tasted his product without testing the purity.

  • @joalsoal1645
    @joalsoal1645 Год назад

    Been wating for someone to try synthesizing this!

  • @ronbaer67
    @ronbaer67 11 месяцев назад +1

    now to test it against pepperX and see which is stronger

  • @kawaiiintelligenceagency3889
    @kawaiiintelligenceagency3889 10 месяцев назад

    This guy actually went mad-scientist enough to actually try it. Cool

  • @petthepizza
    @petthepizza Год назад +2

    I tried to do my own retrosynthesis before watching. Would it have been possible to obtain the fused ring moiety from a 1,2 diphenol derivative via a Pictet-Spengler reaction, then a double condensation with the 4-chlorophenethylamine onto formaldehyde, oxidize to the diamide, and then convert the diamide to the dithioamide via Lawesson’s reagent? I feel like this would massively shorten the synthesis.

    • @nickwade1780
      @nickwade1780 Год назад

      C(sp3)-C(sp2) suzuki coupling could've saved like 4 steps but I guess the corresponding hydroxy-methoxy aryl bromide may be inaccessible. There's plenty of ways to design a more step-efficient route but its probably harder to get ahold of certain chemicals for different routes without being able to blow grant money on commercial reagents.

    • @durshurrikun150
      @durshurrikun150 8 месяцев назад

      @@nickwade1780 Suzuki works well for sp2-sp2 coupling, it doesn't work that well with sp3-sp2 couplings.
      You need electron rich phosphines which are not as easy to handle as triphenyl phosphine

  • @FishyBoi1337
    @FishyBoi1337 Год назад +1

    I feel like this would be better as a solution in mostly water or something with a strong-ish flavor added to mask the other stuff, you just swish and spit it out to get it all through your mouth

  • @Firephilosopher4
    @Firephilosopher4 Год назад +1

    Amazing video! Does someone know which site or app he's using to draw mechanisms?

  • @np6181
    @np6181 Год назад +3

    Would this block the receptors in your eyes so you can both eat it and put it on your face/eyes and make people immune from pepper spray?

  • @regnbuetorsk
    @regnbuetorsk 3 месяца назад

    what an insane amount of steps! i guess it would be easier to just make a new variety of chili that doesn't produce capsicin...

  • @flyingsodwai1382
    @flyingsodwai1382 11 месяцев назад

    Ohhhh. THIS is what it's like to feel dumb. I barely have any idea of what you are talking about but it was fun watching!

  • @sukaisnaini1843
    @sukaisnaini1843 Год назад +3

    Amazing, this is like watching a mage.

  • @nitrgnlab9400
    @nitrgnlab9400 Год назад +1

    Great video! Why don't you use TLC to at least roughly estimate the purity of the obtained compounds?

    • @ambientdiscord
      @ambientdiscord Год назад +1

      He did say he used TLC during 1 step but as an analytical chemist the final steps had me cringing since i purify compounds by HPLC and then check purity on UPLC. If i see like 1% impurity on the chromatogram i feel bad handing it back to the synthetic chemists. I feel like the best thing to do here is something he probably doesn't have access to which would be SFC purification on a cellulose column so the purified compound should have zero toxic residue left behind. I really hate that he used methanol in the final step then tasted it.

  • @basti206
    @basti206 Год назад +2

    take part in hot ones and use this.

  • @Doom2pro
    @Doom2pro Год назад

    Weird Explorer is like, I didn't bother with all those steps, I just got heatless peppers. 😂

  • @STEAMerBear
    @STEAMerBear Год назад +3

    Your willingness to ingest this represents incredible courage* to me.
    (*is courage the right word?)

    • @Poodleinacan
      @Poodleinacan 11 месяцев назад +1

      Trust the process 😂

  • @ArthurBurgan
    @ArthurBurgan Год назад +2

    Wow, I have no idea what's going on here. Somehow got recommended. I'm a dev and can totally relate to this process, in a way, it's engineering. I'm fascinated. So many question :D
    Is it safe to be near this stuff while you're making it? How do the separatory funnel work? How much does this setup cost and is it possible for a regular pleb to just look up stuff and start synthesising things for fun?
    Anyway, makes me want to get an arduino or a raspberry pi and just code something.
    Really WP, understood little, learned a lot.

    • @lunkel8108
      @lunkel8108 11 месяцев назад +3

      "Is it safe to be near this stuff while you're making it?"
      The answer depends on what exactly you mean by that. Nothing here is radioactive, it's not gonna kill you just by being somewhere near you. But in general chemistry can be dangerous, that's why you always follow some basic safety protocols like wearing safety goggles, not getting food near your chemicals, doing most things in a fume hood (which protects you from nasty gasses and also other dangers like splashing or light explosions), etc. You have to respect the chemicals, they can burn you, poison you, explode, etc. If you have a more specific question, I'd be happy to provide more details.
      "How do the separatory funnel work?"
      The piece of glass itself doesn't really do much except hold the liquids. You put in two immiscible (not mixable) liquids with different densities, shake them around so they get in contact with eachother (during this substances can transfer from one liquid to the other depending on their solubility), take care to vent regularly so no pressure builds up and then you just let it sit while the two liquid layers separate due to their different densities. Then you can drain them out one after the other.
      "How much does this setup cost and is it possible for a regular pleb to just look up stuff and start synthesising things for fun?"
      Good glassware is generally not cheap and chemicals can cost quite a lot. Also you definitly need a fume hood and I can't imagine those are cheap to acquire and install. But I'm more concerned about your safety. I don't feel comfortable with the idea of somebody who has no lab experience trying to do organic synthesis unsupervised at their home. You don't really know what's dangerous, how dangerous it is and how to properly protect yourself. And that starts with basic things like properly storing everything.

    • @ArthurBurgan
      @ArthurBurgan 11 месяцев назад

      @@lunkel8108 you're a legend! Thank you for taking the time to respond to me. Yea.. I got a bit too excited as it was the first time I saw something like this and it seemed to cool :D I then quickly realised it's not a good idea to attempt this stuff on my own and I'll just continue on my software engineering path. Ty again for the reply! I learned quite a bit

  • @nekomasteryoutube3232
    @nekomasteryoutube3232 4 месяца назад

    Even though I love spicy stuff, sometimes I wish I could have the flavor and enjoyment of spice without the pain because I tend to find spicy foods quite tasty (and the heat is a treat for me)

  • @djdrack4681
    @djdrack4681 Год назад +1

    This is great to showcase how many molecules are not 'simple'. It also showcases how organic synthesis requires a bunch of harsh molecules (thiophosgene, a furan, etc).
    It also shows scaling is a headache when you want to take a lab synthesis and scale it to industrial production...Now you need tanks for all the chems, sensors, lines that hold/pump the chems (the line can't corrode either), buffer tanks to dump in others case of overheating/emergency (think why Bopal happened). etc.
    Great video and explanation.

  • @WaffleStaffel
    @WaffleStaffel Год назад +4

    Oh neat! Too bad there's not a synthesis for Miraculin.

    • @AllisterCaine
      @AllisterCaine Год назад

      Isn't that the compound that blocks bitter tastes? Appears in a fruit as far as I know.

    • @WaffleStaffel
      @WaffleStaffel Год назад

      @@AllisterCaine It makes sour taste sweet.

    • @AllisterCaine
      @AllisterCaine Год назад +1

      @@WaffleStaffel ah, that's what I was thinking of. Remembered it wrong it seems. Some company sells this stuff as a gimmick in chewing tablets.

    • @WaffleStaffel
      @WaffleStaffel Год назад

      @@AllisterCaine It's a really fun sensation. It makes lemons taste sweet, makes pickles taste like sweet pickles, vinegar taste like sugar water, etc., and it can be used as a non-nutrative sweetener for diabetics and calorie reduction. Big-pharma controlled FDA has failed to approve it for this multiple times, even though it's just a freeze dried fruit. Too much money at stake.

  • @quaraeasaurustex1823
    @quaraeasaurustex1823 3 месяца назад

    Id be interested in seeing how it could possibly be used to treat OC exposure

  • @joshuamcgarry2636
    @joshuamcgarry2636 Год назад

    Why do we have to protect the amine with a benzene group? Why can't we just add NH3 to do the nucleophilic acyl substitution with COOH?

  • @antares8826
    @antares8826 Год назад

    Normally I remove the PPh3O by dissolving the crude in DCM and dumping a shit load of hexanes in it. Since PPh3O isnt soluble in hexane, I can easily decante the supernactant off and continue with that. But this approach looks very nice too, I might give it a try next time

  • @kogure7235
    @kogure7235 Год назад +1

    But the feeling of hellfire in your mouth IS the entire point. That's the fun. There isn't a flavor, it's just the sensation.

  • @dakarpsi
    @dakarpsi Год назад

    I would think making a hard candy out of it would coat the mouth and help cover the flavor

  • @palamalama
    @palamalama Год назад

    Bro, you are very professional, well done

  • @triklettriklerbu1592
    @triklettriklerbu1592 Год назад

    thanks for explaining the reactions. even though i know them its really nice to see mechanisms

  • @ArcaneTinker
    @ArcaneTinker 10 месяцев назад

    What was the time and material cost involved in this sequence? There was so much solvent and washing used. Genuinely curious as someone who is fascinated with organic chemistry yet I am completely baffled by it as well. I feel like a little toddler listening to grown-ups having a deep conversation.

  • @Thaumius
    @Thaumius Год назад

    Do you do NMR of your final products?

  • @DanskerneFraDanmark
    @DanskerneFraDanmark 7 месяцев назад

    Is there any chemical reaction that requires sound?

  • @ZachTheZOG
    @ZachTheZOG Год назад

    Bro really just said "Yo let me batch up some fire resistance elixars real quick" 🔥🔥🔥

  • @jhhwild
    @jhhwild 3 месяца назад

    I thought the opposite of spice was mint or menthol, like you get that cool feeling rather than the heat feeling, but both can be pretty intense.

  • @YoKnow
    @YoKnow 11 месяцев назад

    I don't think I can go a day without spicy! A better solution is to figure out if the bond is hydrophobic. Also regardless if it is or not and by your testimony it isn't. Use the solution as a mouth wash instead of just putting a drop in one specific spot on your buds! Enjoy!

  • @philidor9657
    @philidor9657 2 месяца назад

    "DCM/MeOH columns are cursed"
    Pretty much, yeah. They either work way better than you hoped, or don't work at all. I've done so many DCM/MeOH columns because the separation by TLC was awesome, but on a column pretty much everything just rips through. I've learned to just stop trying DCM/MeOH columns unless there's no other option.

  • @TheDeepDiveLLC
    @TheDeepDiveLLC 3 месяца назад

    In addition to this, you could just climb up the ladder until your body no longer reacts to it

  • @douro20
    @douro20 7 месяцев назад

    The CMMTPP was probably the most expensive reagent used here...

  • @LowdownBoy
    @LowdownBoy 8 месяцев назад

    If you're really interested in enjoying spicy foods and it's not just a vehicle for your video concept, then might I suggest an easier method; all you do is start small with jalapeno, then gradually work your way up to say a Cayenne, to Serrano, etc., making your way up the Scoville ladder. Once you hit a certain level, enjoy the burn for it's effects, then keep going once it becomes "nothing" to you. Do all of this with food. Every substantial meal you have, enjoy a fresh pepper or mash alongside.

    • @LowdownBoy
      @LowdownBoy 8 месяцев назад

      I love it. Peppers especially fresh, have some of the most complex flavor profiles of any flower. This is especially true for the super-hots. I eat Carolina Reaper powder in my soups and with chicken noodle soup, you can't pick a better spice to pair with a nice yellow curry.

  • @ThePickledsoul
    @ThePickledsoul Год назад +1

    I wonder if it works on eyes. Could nullify pepper spray

  • @Pancreaticdefect
    @Pancreaticdefect Год назад +2

    I watch a lot of chemistry videos on RUclips and I have come to the conclusion that you guys must buy DCM by the tanker truck.

  • @dcm2430
    @dcm2430 Год назад

    Very cool synthesis!

  • @afronprime51
    @afronprime51 Год назад

    It would solve another problem as well if you were to use it as a rectal application. Protection for the day after.

  • @kleetus92
    @kleetus92 Год назад

    I laughed out loud at the Drawn Together tunnel reference! God, that show was so wrong it was hilarious...

  • @manganstein
    @manganstein 3 месяца назад +1

    I synthesized compound SB-366791 (CAS: 472981-92-3), and it works very well, it completely inhibits sense of spiciness but doesn’t have unpleasant taste / after taste (at least in some dilution), only side effect I noticed is weak sensation of anaesthesia / numbness on the tongue, and maybe a little disruption in taste in general (I am not sure if its effect taste itself or its just weird sensation of numbness mess with taste sensation). Only problem I have is with application as compound is insoluble in water, in first tires I “dilute” it by grinding with sugar - what works not perfectly, then I attempt to make caramel candy with this compound and for now it works quite well - while licking the candy distributes compound well in mouth and sensation of spiciness (very weak) only appear in the back of the throat, tested on carolina reaper.

    • @hawaiianprestigecars8493
      @hawaiianprestigecars8493 2 месяца назад

      How can I contact you ? Gotta ask you something

    • @manganstein
      @manganstein 2 месяца назад

      ​@@hawaiianprestigecars8493I recently added mail adress for YT acount.

  • @Felixkeeg
    @Felixkeeg Год назад +5

    Bro completely raw-dogged this synthesis

  • @douro20
    @douro20 7 месяцев назад

    I guess a 1L separatory funnel is too unwieldy?
    I'd be nervous adding Pd/C to a flammable liquid, even if it were Pd(OH)2/C or Pd(OAc)2/C instead.

  • @cwyvern4861
    @cwyvern4861 3 месяца назад

    Would menthol work? Capsaicin = Spicy, Menthol = Cold, so would Capsaicin + Menthol = Neutral?

  • @TheHuntermj
    @TheHuntermj 3 месяца назад

    I wonder if you could replace some of those hydrogens with Fluorine and make it a bit more permanent?

  • @rwbrandom1252
    @rwbrandom1252 11 месяцев назад

    Would it not have been easier to work backwards from the spicy chemical ?

  • @BramCohen
    @BramCohen Год назад

    Somebody needs to use this to win a chili eating contest

  • @Fili229
    @Fili229 Год назад

    I can't understend, why do you don't use nmr?