So fascinating. In the late 60s as a high-schooler I spent hours looking at protozoa and simple multicellular organisms. Thanks! Brought back memories.
The image quality you get in these videos is every bit on par with any other channel on RUclips, Oliver, it's amazing! The clip at the end is the sharpest I have ever seen the internal details of the rotifer. Every time I watch I feel I must chain my wallet away lest I overindulge in another expensive hobby.
Lovely, That was one off the most interesting explanations you've done in a long time. More off this pleas5. Thank you very much Sir. Cheers from Sweden
Thank you for this feedback. This is important. I do sometimes have trouble finding what people are interested in. I was already worried, that the discussion of refractive index etc. is already too specific and technical.
Have you tried staining onion cells with potassium permanganate? I used it with ethanol and water instead of using ink, it stained nuclei but also other structures that I couldn’t identify
waaaaw, MH, this is very interesting news to me, the line that is not a line but a difference in refr index! 1. phase contrast seems the best rendition of this fact // 2. so that's why we can see bacteria better with Ph Co // 3. then maybe 'texture' is a way to find groups of cells with a similar job 3. if P. bursaria always has algae, it becomes de facto a multicellular animal (like mitochondrion became part of cells). fascinating. thank you
also, the visibility of the algae inside P. bursaria is not only color, but being a plant, it will have a visible wall? oooh, you got my head spinning now
To be precise, the dark line around the cells is due to the diffraction of light (due to a difference in refractive index). When you look at an air bubble under the microscope, then you will also see that it has a very dark (and thick) line. This is also due to the difference in refractive index between water and air.
@@Microbehunter ich verstehe ihen Komentar garnicht...Die Methoden PH und DIC sind auch nur Varianten einer Hellfeldmikroskopie mit denen man tierische Zellmembranen durchaus darstellen kann
So fascinating. In the late 60s as a high-schooler I spent hours looking at protozoa and simple multicellular organisms. Thanks! Brought back memories.
Glad you enjoyed it!
Excellent short video and very informative Thank you, Oliver!
Great video, really interesting!
Thank you!
Thank you for sharing your knowledge! It was very informative to see in real-time the differences in Eukaryotic and Prokaryotic cells.
Yes, this video is very interesting!😃🙃
Glad it was helpful!
The image quality you get in these videos is every bit on par with any other channel on RUclips, Oliver, it's amazing! The clip at the end is the sharpest I have ever seen the internal details of the rotifer. Every time I watch I feel I must chain my wallet away lest I overindulge in another expensive hobby.
A very nice presentation. And I definitely learned something new. Nice images as well.
Glad to hear it! Thank you!
I truly love your videos!
Glad you like them!
Excellent thank you. Explained it so even I could understand it. Like the water in the glass analogy to explain refractive index.
Glad it was helpful!
Too interesting if you ask me, both visual and informative, love it!
Awesome, thank you!
Lovely, That was one off the most interesting explanations you've done in a long time. More off this pleas5. Thank you very much Sir. Cheers from Sweden
Thank you for this feedback. This is important. I do sometimes have trouble finding what people are interested in. I was already worried, that the discussion of refractive index etc. is already too specific and technical.
@@Microbehunter I find those topics interesting.
Learning something everyday, very good explanations too.
Glad to hear that!
Great video, thanks for the explanation.
Glad you enjoyed it!
Have you tried staining onion cells with potassium permanganate?
I used it with ethanol and water instead of using ink, it stained nuclei but also other structures that I couldn’t identify
Wonderful!
waaaaw, MH, this is very interesting news to me, the line that is not a line but a difference in refr index! 1. phase contrast seems the best rendition of this fact // 2. so that's why we can see bacteria better with Ph Co // 3. then maybe 'texture' is a way to find groups of cells with a similar job 3. if P. bursaria always has algae, it becomes de facto a multicellular animal (like mitochondrion became part of cells). fascinating. thank you
also, the visibility of the algae inside P. bursaria is not only color, but being a plant, it will have a visible wall? oooh, you got my head spinning now
To be precise, the dark line around the cells is due to the diffraction of light (due to a difference in refractive index). When you look at an air bubble under the microscope, then you will also see that it has a very dark (and thick) line. This is also due to the difference in refractive index between water and air.
@@Microbehunter amazing
It could be interesting to label the nuclei with DAPI o another molecule.
What magnification is that?
Different ones, maximum objective was 60x, so these organisms can be seen with conventional compound microscopes.
👍
why you talk about refractive index, color and staining since everybody has phase-contrast and DIC
your comments are annoying
The same concepts also apply to brightfield microscopy.
@@Microbehunter if you dont see details wich have the same refractiv index then use PH or DIC even darkfield helps
@@sannee3715 das ist mir sowas von egal
@@Microbehunter ich verstehe ihen Komentar garnicht...Die Methoden PH und DIC sind auch nur Varianten einer Hellfeldmikroskopie mit denen man tierische Zellmembranen durchaus darstellen kann