Spore Stain Procedure (Schaeffer-Fulton Method)
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- Опубликовано: 2 окт 2024
- Bacterial Endospore stain procedure. This protocol is taken from "Benson's Microbiological Applications: Laboratory Manual in General Microbiology" Eleventh Edition
1. Cover the bacteria smear with a piece of paper towel and saturate it with malachite green. Steam it over boiling water for 5 minutes and add more stain as it boils off.
2. Allow the slide to cool, remove the piece of paper towel and rinse for 30 seconds.
3. Counterstain with safranin for about 20 seconds.
4. Rinse briefly with water to remove safranin
5. Blot dry and view under the microscope in immersion oil.
Camera: Nikon D3300
Microscopes:
Leica ATC 2000 - 1000X
American Optical One - Ten microscope - 1500X
A lot of people will be disappointed when the first aliens we discover will most likely be bacteria.
I will be excited.
Not this guy
We probably won’t exist to see it but that would be fascinating!
I wanna see bacteria from space!
We already did, moon called europa
Its like 3AM, and im looking on these micro bois.
Worth it.
Where you from?
@@emanuelc.3589 Ukraine.
@@orkboy1621 weird way to say Char
@@Holyn579 Most of these little terrans will not even understand what is it.
@@orkboy1621 Perfection. Deep in the core, in strands.
Can you make one with dog saliva? Do they have any harmful bacteria?
Rodo -Troll how about human saliva
Rodo -Troll std Std STd STD!
Hey are you studying biology or something, or you are maybe a scientist? :D Love your videos!
I am a biology lab tech and a part time professor.
I *never* comment on youtube videos but need to say thank you! This paper towel trick is saving me from a huge mess and nasty malachite green steam.
You mention in this video that the spores are resilient to many things, radiation among them; but recalling back to your UV light on E. Coli demonstration, would these spores also be resistant to the effects of UV, or would the light still manage to form the thymine dimers as I think you called them? Assuming it can't survive, I wonder if there's any species that could live and reproduce under constant high-level UV exposure.
Absolutely, we just aren't aware of them.
Spores are resistant to UV as well. I am making another video comparing spores vs. regular bacteria when it comes to boiling, UV, etc.
I'm so glad I subbed.
Looking at things under the microscope is one of the things I miss most from school. And I didn't even realize that until I saw your channel recently.
Hi! Do you make the smears from the 48-hour broth culture or from colony on agar plate? I did this using a 48 hour bacillus subtilis culture on agar plate but I couldn't see any spores. Does it only work for cultures grown in broth?
Both both and agar work. Spores form in order to survive harsh conditions. So older cultures have more spores (I make some cultures 48 hours to a week old). Dehydration and low and high temps can also induce sporulation.
Thank you for the nice video showing the protocol. I have a small question.
Towards the end, after the staining, do you use a cover glass before adding immersion oil and investigating the sample under the microscope? Or do you directly apply the immersion oil to the stained sample on the microscope glass and use it for microscopy?
I don't use a coverslip, but you can. I know a professor that preferred to use a coverslip.
Do you have an email address I'd be able to contact you on, I have a few questions about Planaria and you are clearly very educated on it, so would I be able to please contact you?
You can contact me at Sci.inspi@gmail.com. I am not an expert in planaria but I can try to answer whatever questions you might have.
Wow! It's just wowwww! The way it's shown here is just amazingggg :))
Hey mate. Your videos are great, but anyway I'd like to suggest you to try using more ambient/silent songs. Viewing you doing those procedures is a somewhat relaxing experience but the agitated background songs often contrast with that feeling. Also, sometimes they just take your attention, because they sound interesting. That's all I have to suggest. For instance this nice jazzy guitar solo often snapped me out of the legends lol.
Lol I know what you mean. I tried to make this one pretty tame but I guess it wasn't low enough.
Ivlo experiment eppadi mind la yabagapaduthi vachikkirathu ? Results eppadi kandupidikkirangala kashtama irrukkuma in tha course?
can you eat it???
Nope, it would get you sick.
@@sci-inspi but would it be tasty
Im happy to see youre still doing this!
I'm still here lol.
Nice
Excellent work, I am one of the channel subscribers, I recommend you share the video with reference to the source and save the rights of your property
I included the reference source in the description.
@@sci-inspi I wait for your approval, and my channel is very simple and not for the purpose of making money
@@labzool9235 Are you asking to use some of my footage?
@@sci-inspi Yes and I translate it into Arabic with reference to you (certainly some)
@@sci-inspi I am from a country with few potentials and I invite you to subscribe to my channel and direct my benefit to my simple students
Would it be possible to see if you could put urticating bristles from a tarantula under the microscope.
nice
Can we see river or “dirty” water filtered through a life straw or something under a microscope comparisons? I think that would be cool!
I'm curious whether you play all of these BGMs in your clips.
can u film what happens when u add active carbon to foul water?
With source. EPIC.
I have homework, but instead I’m here.
You should do one with grass blades or dirt
Any Angeles students here? xD
At the end, were the spores alive?
I am not sure, I heat fix the slide with a bunsen burner. I don't know if that heat is enough to kill them.
@@sci-inspi Okay. Also one question, what microscope do you use?
good veiw of spore staining
Please do more Gram stains! ASMR Gram stains!!!
Como mierda llegue aquí
This channel is amazing. I wish I found it sooner. Keep up the fantastic work.
I miss my microbiology lab ;__;
Good video like always
Very good video 👍🏻
I like the music better than the video
Thank you 🤗
Hello, do you still upload to SoundCloud?
Not lately, I am using a new service that uploads to dozens of music services but not sound-cloud.