Spore Stain Procedure (Schaeffer-Fulton Method)

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  • Опубликовано: 24 дек 2024

Комментарии • 77

  • @orkboy1621
    @orkboy1621 5 лет назад +80

    Its like 3AM, and im looking on these micro bois.
    Worth it.

    • @emanuelc.3589
      @emanuelc.3589 5 лет назад +1

      Where you from?

    • @orkboy1621
      @orkboy1621 5 лет назад +1

      @@emanuelc.3589 Ukraine.

    • @Holyn579
      @Holyn579 5 лет назад +1

      @@orkboy1621 weird way to say Char

    • @orkboy1621
      @orkboy1621 5 лет назад +1

      @@Holyn579 Most of these little terrans will not even understand what is it.

    • @astilen5647
      @astilen5647 5 лет назад

      @@orkboy1621 Perfection. Deep in the core, in strands.

  • @AdlinLing
    @AdlinLing 5 лет назад +72

    A lot of people will be disappointed when the first aliens we discover will most likely be bacteria.

  • @kearagrady8975
    @kearagrady8975 2 года назад +3

    I *never* comment on youtube videos but need to say thank you! This paper towel trick is saving me from a huge mess and nasty malachite green steam.

  • @Karibiane
    @Karibiane 5 лет назад +3

    I'm so glad I subbed.
    Looking at things under the microscope is one of the things I miss most from school. And I didn't even realize that until I saw your channel recently.

  • @Jacob-sb3su
    @Jacob-sb3su 5 лет назад +1

    Im happy to see youre still doing this!

  • @rodo-troll8084
    @rodo-troll8084 5 лет назад +20

    Can you make one with dog saliva? Do they have any harmful bacteria?

  • @Zimidiah
    @Zimidiah 5 лет назад

    This channel is amazing. I wish I found it sooner. Keep up the fantastic work.

  • @souporfreaky
    @souporfreaky 5 лет назад +5

    You mention in this video that the spores are resilient to many things, radiation among them; but recalling back to your UV light on E. Coli demonstration, would these spores also be resistant to the effects of UV, or would the light still manage to form the thymine dimers as I think you called them? Assuming it can't survive, I wonder if there's any species that could live and reproduce under constant high-level UV exposure.

    • @AudioStorm1980
      @AudioStorm1980 5 лет назад

      Absolutely, we just aren't aware of them.

    • @sci-inspi
      @sci-inspi  5 лет назад +1

      Spores are resistant to UV as well. I am making another video comparing spores vs. regular bacteria when it comes to boiling, UV, etc.

  • @labzool9235
    @labzool9235 5 лет назад +1

    Excellent work, I am one of the channel subscribers, I recommend you share the video with reference to the source and save the rights of your property

    • @sci-inspi
      @sci-inspi  5 лет назад +1

      I included the reference source in the description.

    • @labzool9235
      @labzool9235 5 лет назад +1

      @@sci-inspi I wait for your approval, and my channel is very simple and not for the purpose of making money

    • @sci-inspi
      @sci-inspi  5 лет назад +1

      @@labzool9235 Are you asking to use some of my footage?

    • @labzool9235
      @labzool9235 5 лет назад

      @@sci-inspi Yes and I translate it into Arabic with reference to you (certainly some)

    • @labzool9235
      @labzool9235 5 лет назад

      @@sci-inspi I am from a country with few potentials and I invite you to subscribe to my channel and direct my benefit to my simple students

  • @shivanisharma1516
    @shivanisharma1516 3 года назад +1

    Wow! It's just wowwww! The way it's shown here is just amazingggg :))

  • @sufiasani
    @sufiasani Год назад +1

    Hi! Do you make the smears from the 48-hour broth culture or from colony on agar plate? I did this using a 48 hour bacillus subtilis culture on agar plate but I couldn't see any spores. Does it only work for cultures grown in broth?

    • @sci-inspi
      @sci-inspi  Год назад

      Both both and agar work. Spores form in order to survive harsh conditions. So older cultures have more spores (I make some cultures 48 hours to a week old). Dehydration and low and high temps can also induce sporulation.

  • @Fab4Gamers
    @Fab4Gamers 5 лет назад

    Can we see river or “dirty” water filtered through a life straw or something under a microscope comparisons? I think that would be cool!

  • @watthederp
    @watthederp 5 лет назад

    Would it be possible to see if you could put urticating bristles from a tarantula under the microscope.

  • @JOJO7130
    @JOJO7130 5 лет назад +2

    Thank you for the nice video showing the protocol. I have a small question.
    Towards the end, after the staining, do you use a cover glass before adding immersion oil and investigating the sample under the microscope? Or do you directly apply the immersion oil to the stained sample on the microscope glass and use it for microscopy?

    • @sci-inspi
      @sci-inspi  5 лет назад +1

      I don't use a coverslip, but you can. I know a professor that preferred to use a coverslip.

  • @RedRaiRobot
    @RedRaiRobot 5 лет назад

    You should do one with grass blades or dirt

  • @kurth6140
    @kurth6140 5 лет назад

    I'm curious whether you play all of these BGMs in your clips.

  • @superjojo555
    @superjojo555 5 лет назад

    can u film what happens when u add active carbon to foul water?

  • @gowriselvam1718
    @gowriselvam1718 2 года назад

    Ivlo experiment eppadi mind la yabagapaduthi vachikkirathu ? Results eppadi kandupidikkirangala kashtama irrukkuma in tha course?

  • @naoyeee
    @naoyeee 5 лет назад +1

    At the end, were the spores alive?

    • @sci-inspi
      @sci-inspi  5 лет назад

      I am not sure, I heat fix the slide with a bunsen burner. I don't know if that heat is enough to kill them.

    • @naoyeee
      @naoyeee 5 лет назад

      @@sci-inspi Okay. Also one question, what microscope do you use?

  • @Mustang_99
    @Mustang_99 5 лет назад +9

    Hey are you studying biology or something, or you are maybe a scientist? :D Love your videos!

    • @sci-inspi
      @sci-inspi  5 лет назад +8

      I am a biology lab tech and a part time professor.

  • @abeenfatima3493
    @abeenfatima3493 3 года назад

    Very good video 👍🏻

  • @rayres1074
    @rayres1074 5 лет назад +1

    Hey mate. Your videos are great, but anyway I'd like to suggest you to try using more ambient/silent songs. Viewing you doing those procedures is a somewhat relaxing experience but the agitated background songs often contrast with that feeling. Also, sometimes they just take your attention, because they sound interesting. That's all I have to suggest. For instance this nice jazzy guitar solo often snapped me out of the legends lol.

    • @sci-inspi
      @sci-inspi  5 лет назад

      Lol I know what you mean. I tried to make this one pretty tame but I guess it wasn't low enough.

  • @milk8928
    @milk8928 5 лет назад

    Please do more Gram stains! ASMR Gram stains!!!

  • @tubamalik9232
    @tubamalik9232 2 года назад

    good veiw of spore staining

  • @jasperrockburn293
    @jasperrockburn293 5 лет назад +1

    Do you have an email address I'd be able to contact you on, I have a few questions about Planaria and you are clearly very educated on it, so would I be able to please contact you?

    • @sci-inspi
      @sci-inspi  5 лет назад

      You can contact me at Sci.inspi@gmail.com. I am not an expert in planaria but I can try to answer whatever questions you might have.

  • @TheFire259
    @TheFire259 5 лет назад +7

    can you eat it???

  • @Username-or9nr
    @Username-or9nr 5 лет назад

    Good video like always

  • @wilfredogomez5505
    @wilfredogomez5505 5 лет назад

    I have homework, but instead I’m here.

  • @0zetazetazeta0
    @0zetazetazeta0 5 лет назад +1

    Nice

  • @Gerrit2686
    @Gerrit2686 11 месяцев назад

    With source. EPIC.

  • @anishrahulc9188
    @anishrahulc9188 5 лет назад +1

    nice

  • @swindle3561
    @swindle3561 5 лет назад

    I miss my microbiology lab ;__;

  • @mervesenol524
    @mervesenol524 5 лет назад

    Thank you 🤗

  • @ddaabbzz187
    @ddaabbzz187 5 лет назад

    I like the music better than the video

  • @Bazanguitar
    @Bazanguitar 5 лет назад

    Como mierda llegue aquí

  • @eulashrimp5352
    @eulashrimp5352 3 года назад

    Any Angeles students here? xD