W31: Spatial Transcriptomics - Day 1
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- Опубликовано: 5 авг 2024
- Spatial transcriptomics is an emerging field that bridges molecular biology and anatomy. Over the last decade, a battery of assays have been developed that profile gene expression in-situ, i.e, measuring the abundances of mRNA molecules in cells and tissues while retaining information about their spatial locations. This workshop will introduce the basic concepts, major techniques, and typical analysis workflows in spatial transcriptomics. It will also offer guided hands-on coding exercises for the analysis of public spatial transcriptomic datasets.
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Great workshop! Thanks a lot!
Great work! Thank you!
Brilliant. Thanks a lot
Could you provide more detailed info regarding the publication @31:21? Michael Nunn, 2020. I can't find the orginal article.
Nice job! but quick question I am Ph.D. student in the pharmacology and toxicology department with a pharmacy degree background wanted to know how feasible it is to learn transcriptomes in 6 months internship?
Great! One observation, we cant do double, triplo fish, Fish and immunofluorescence etc.
When you do multiple rounds of smFISH, are you moving the slide from the microscope to the bench and back again after each round? How are you able to preserve the same position when you overlay the multiple images? A small shift of the slide placement could result in a shift image, right? How is this overcome?
There is no need to remove the slide every round. In the present comercial platform using MERFISH, the slide is set in a flow chamber, and the different probes will fluid in automatically after the former round's imaging. Also the objective lens will also automatically move to the next FOV.
what's the difference between multiplexing vs throughput?
Multiple rounds vs number each round.
ruclips.net/video/URqjNcZ7d5E/видео.html
@@xiaohuiyu1928 It's lovely to see that you came back and answered your own question (I mean that genuinely). I am aware that throughput usually refers to the number of cells that the technique can handle; in scRNA-Seq, my area of focus, droplet-based methods are very high throughput but they lack sensitivity whereas plate-based methods are exactly the opposite (high sensitivity and low throughput).
What would multiplexing mean in this context though? I would appreciate your input.
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