ADS1: Base calling and sequencing errors

This is one if the optional lectures in the series. Here we discuss how sequencing by synthesis can make mistakes, and how software tools called base callers attempt to determine each base and characterize the uncertainty of each call. Course page: www.coursera.o....

[@masoudnamini1018]

thanks. much clearer than my professor!!

[@CuChulain0415]

Thanks. much clearer than my professor!! +1

[@verarykalina3728]

I am afraid you’ve got a mixture of two different technologies here: Helicos and Illumina.

[@pbehrouz]

This is Great please Do more courses in this Area DNA sequencing. more algorithms

[@sanjuvikasini1598]

The explanation was crystal clear! Thanks for the video sir 🙂

[@hermainrais2280]

Make more videos you really clear my concept 😊

[@farrahkhan4091]

this content is incredible. Really appreciate that you developed it and shared it.

[@sonivijay6097]

Thank you so much! Great explanation, this solved my no. of doubts!

[@zahraafakih7020]

When you mentioned about amplification of the original template, here during this process aren't we goin' to get complementary sequences? How all the clones of the original templates have same and not complementary sequences upon PCR?

[@danielherrera1458]

Do we know how many bases our strands will have in advance.
I'm wondering this because if this is the case, can count the number of snapshots and have other ideas on quality counting what bases appears to end stacking new bases first, which could give us an idea on which ones are out of sync.
Is this possible?

[@yianniszografakis652]

Well done and thank you very much. Couldn't find the last bit you explained anywhere else, and you explained very clearly as the rest of the process. Keep going.!

[@serhat757]

Excellent explanation! Thanks!

[@yianniszografakis652]

Well done and thank you very much. Couldn't find the last bit you explained anywhere else, and you explained it very clearly as the rest of the process. Keep going.!

[@khalidakram]

Nice video on quality