BioXTAS RAW - Evolving factor analysis (EFA) for SEC-SAXS data

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  • Опубликовано: 11 фев 2025
  • This tutorial video teaches you how to do evolving factor analysis (EFA) in BioXTAS RAW to deconvolve overlapping peaks in size exclusion chromatography coupled small angle x-ray scattering (SAXS) data (SEC-SAXS). It goes through part of the tutorial described here:
    bioxtas-raw.re...
    The tutorial assumes a basic working knowledge of processing SEC-SAXS data in RAW.
    More tutorial videos are available here:
    • BioXTAS RAW Tutorials
    BioXTAS RAW is a GUI based, free, open-source Python program for reduction and analysis of small-angle X-ray solution scattering (SAXS) data. The software is designed for biological SAXS data. It is available on windows, macOS (and OS X), and linux. It provides an alternative to closed source programs such as Primus and Scatter for primary data analysis. Because it can calibrate, mask, and integrate images it also provides an alternative to synchrotron beamline pipelines that scientists can install on their own computers and use both at home and at the beamline.
    The software enables: creation of 1D scattering profiles from 2D detector images, standard data operations such as averaging and subtraction, analysis of radius of gyration (Rg) and molecular weight, and advanced analysis using GNOM and DAMMIF as well as electron density reconstructions using DENSS. It also allows easy processing of inline SEC-SAXS data and data deconvolution using the evolving factor analysis (EFA) method.
    Learn more about RAW here:
    bioxtas-raw.re...

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