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Gene Set Enrichment Analysis| GSEA algorithm

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  • Опубликовано: 6 авг 2024
  • After not being able to find a good video or website explaining the whole mechanism of GSEA without over using mathematics and stuff. I spent about the day, and this is the outcome. Despite not being necessary to understand the background algorithm behind the tools we use, I really don't feel conformable interpreting a data that were spit out to me by an algorithm without understanding how the data is being generated.
    Host: Brandon Yeo
    Writer: Brandon Yeo
    Editor: Brandon Yeo
    Running GSEA in R
    • Gene Set Enrichment An...
    Slides
    docs.google.com/presentation/...
    Excel file
    github.com/brandonyph/LiquidB...
    Resources
    1. gsea.org/GSEA
    2. www.pathwaycommons.org/guide/...
    3. pubmed.ncbi.nlm.nih.gov/16199...
    Email: liquidbrain.r@gmail.com
    Website: www.liquidbrain.org/videos
    Patreon: / liquidbrain
    Chapters
    0:00 Introduction
    0:20 The two parts
    0.46 The over representation analysis
    1:39 The problem with Cut off in DEGs isolation
    2:41 The solutions?
    3:14 Example of some GSEA
    4:00 This is a simpler version of the algo
    4:20 The 4 main step
    5:15 More examples
    5:33 Step 1- Enrichment calculation
    9:15 Step 2 - KS test
    11:53 Step 3 - BH correction
    14:50 Step 4 - Permutation test

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