How to Resize Crop and adjust Brightness or Contrast in multiple images using ImageJ

The macro is in the comments section below.
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[@nrttaye4033]

Here is the macro to save individual image files into tiff
dir = getDirectory("");
fileList = getFileList(dir);
output_dir = dir + File.separator + "" + File.separator ;
File.makeDirectory(output_dir)
ch_nbr = nImages ;
for ( c = 1 ; c

[@yogeshbhargava8580]

This is what I was looking for as the raw images were very big and downgrading it via other methods compromised the quality.... Once again thank you very much for this step by step protocol via clarity.... you deserve 10000s applause for all your videos..... keep the good work....
If possible, if you can make an updated video for 2D image deconvolution of fluorescence images.... I have seen your earlier videos but seems that the plug-ins are not working in windows 11 or the sites are down for downloading other plug-ins....
It would be a great help for me with your updated video on 2D image deconvolution.... 😊

[@nrttaye4033]

Hello @yogeshbhargava8580, Thank you so much for your warm words. I am delighted to hear you found the video very useful. If you have any questions or would want to see something particular in future videos, please let me know. Your support matters a lot. I will get back to you once I figure out with the deconvolution part.

[@jamilaiqbal202]

Can you also include the brightness and contrast setting in this macro ?

[@nrttaye4033]

Hello here is the macro you asked for. users would have to change the SetMinAndMax values accordingly.
//run("Brightness/Contrast...");
setMinAndMax(4, 146);
run("Close");
dir = getDirectory("");
fileList = getFileList(dir);
output_dir = dir + File.separator + "" + File.separator ;
File.makeDirectory(output_dir)
ch_nbr = nImages ;
for ( c = 1 ; c

[@Kangkansadiya]

thanks for the informative video

[@nrttaye4033]

You're welcome