Ask a Scientist: How does the 20% rule help manage expectations in targeted NGS?

What is the 20% rule in NGS Target Enrichment? This video explains why sequencing to a coverage depth greater than 20% of the theoretical maximum coverage for a sample may lead to an increased rate of PCR duplicates. It also describes how each aspect of the library prep workflow-including shearing method, kit choice, size selection, and multiplexing scheme-can influence the accuracy of final coverage estimates.
Presented by Teri Rambo Mueller, Field Applications Consultant at Roche Sequencing and Life Science.
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