After completing batch. I integrate standard and save method. I use this method file to process complete batch with data processing and it processed all data. But when I open file in browser window for statistical report. Some of the files do not show area 0 values. Some data files show results but mostly files with zero area and - - values.
@@labsoln when I integrate individually data files. Still it show no results in browser window. The effect of integration in post run is not reflecting in browser window.
@@labsoln we are using shimadzu 8050 when we start to set MRM for mixture of standard of compounds of the same groups with concentration of 1 ppm we don't get all the components optimised some of them don't apper in the final method for example a mixture of tetracyclines standards contain oxytetracycline , tetracycline, doxy tetracycline,and chlortetracycline with final volume of 1ppm when we set the mrm usually we get the result of 2 or 3 out of the 4 please help me identify the couse of this issue we tried deferent compounds different concentrations and still we have some compound missing at the final results. I will appreciate your answer Thank in advance
Thanks for query!, Kindly follow below suggestion: Please decrease the the concentration of tetracylines standards .you need to use 500 ppb std conc to optimize the voltage , precursor ion, product ions. In method optimization, select the option " optimization voltage" where you can fix precursor & product in method. Kindly do this changing definately your method will become good. If you have any further query, you can reach directly on my mobile no.-9384809996 . www.shimadzu.com/an/literature/hplc/52_uflc_applicationdatasheet.html
After completing batch. I integrate standard and save method. I use this method file to process complete batch with data processing and it processed all data. But when I open file in browser window for statistical report. In post run data show result but the same data show zero values in browser window for statistical report.
sir how to calculation of peak to valley ratio
Can we download this program in personal labtop (for manage the post run data)?
Hoe to choose co_injection in old lc solution
I have lc 20
How will you go for next chromatogram
After completing batch. I integrate standard and save method. I use this method file to process complete batch with data processing and it processed all data. But when I open file in browser window for statistical report. Some of the files do not show area 0 values. Some data files show results but mostly files with zero area and - - values.
Check RT value added during integration.
@@labsoln when I integrate individually data files. Still it show no results in browser window. The effect of integration in post run is not reflecting in browser window.
Please make a video on dual wavelength integration
Ok. Shall do it soon.
@@labsoln i need physical training please provide contact number
@@labsoln in PDA detector
Why no peak show's me on chromatogramm
Contact Shimadzu Representative.!
Pleeeeeease do an English version of this videos
Noted
@@labsoln please i your need help with ms.
@@labsoln we are using shimadzu 8050 when we start to set MRM for mixture of standard of compounds of the same groups with concentration of 1 ppm we don't get all the components optimised some of them don't apper in the final method for example a mixture of tetracyclines standards contain oxytetracycline , tetracycline, doxy tetracycline,and chlortetracycline with final volume of 1ppm when we set the mrm usually we get the result of 2 or 3 out of the 4 please help me identify the couse of this issue we tried deferent compounds different concentrations and still we have some compound missing at the final results.
I will appreciate your answer
Thank in advance
Thanks for query!,
Kindly follow below suggestion:
Please decrease the the concentration of tetracylines standards .you need to use 500 ppb std conc to optimize the voltage , precursor ion, product ions. In method optimization, select the option " optimization voltage" where you can fix precursor & product in method.
Kindly do this changing definately your method will become good.
If you have any further query, you can reach directly on my mobile no.-9384809996 .
www.shimadzu.com/an/literature/hplc/52_uflc_applicationdatasheet.html
@@labsoln thank you very verrry very much i well fellow your advice and i will get back to you if i needed more help 🙈
Plz make a video for how to download lab solution software
You can contact Shimadzu Representative as it is not free.
How do download software
Contact Shimadzu Representative
Sir please 3d picture
Vedio is not clear
clear sir
Where you have doubt?
At intrigation time
Ok Mam. We noted that.
@@labsoln sir plz clear this concept
After completing batch. I integrate standard and save method. I use this method file to process complete batch with data processing and it processed all data. But when I open file in browser window for statistical report. In post run data show result but the same data show zero values in browser window for statistical report.
You are not explaining in detail