PCR primer analysis

0:46 Describing the DNA double helix cartoon example
1:46 The role of primers in PCR and their structure
3:13 Template and primer strand polarities
3:45 Template strands
3:54 Definition of oligonucleotide
5:21 Primers are designed to be complementary to the template strands
6:01 PCR amplification process (briefly)
7:26 Primer sequences should be unique in the template sequence
12:48 Forward and reverse primer nomenclature
13:15 Three requirements of quality primer pairs
14:21 Nucleotide coordinate numbering in a chromosome sequence
18:34 Top and bottom (and plus and minus) strand nomenclature
23:07 Using the BLAST website to perform a sequence search
23:50 Query sequence definition
23:56 FASTA sequence format description
25:58 FASTA search results interpretation
27:30 Subject sequence definition
32:18 Deciding which subject matches to view
36:06 Summary of the BLAST results interpretation
36:37 Diagramming the chromosome coordinates of the primers
38:24 Summary of the primer analysis
39:03 Calculating the expected length of a PCR product
39:44 Summary

[@sandeepvivek81]

although it is a long video, but its worth it, very informative

[@dr.hanifurrahman8183]

Perfectly described sir

[@mrmeach1967]

22:47
Blast explained