Onion Skin Epidermal Cells: How to Prepare a Wet Mount Microscope Slide

Step-by-step video and audio instructions on how to prepare a wet mount specimen of onion bulb epidermis plants cells.Video includes explanation of microscope concepts of "parfocal" and "depth of field".
By Tami (Guy) Port, Chief Executive Nerd at ScienceProfOnline. For the lab materials that go with this movie, go to the Virtual Microbiology Classroom Microscopy Laboratory Main Page at ScienceProfOnline.com.

[@owainrose4556]

Wow this is some high quality visual effects.

[@andrewvu3932]

This video has helped me pass my experiment, at first I didn't know how to get the Epidermal layer out of the onion. I kept destroying my layer with my scalpel knife. After watching the video in close detail with the technique on pealing the layer by cutting the onion downwards and using tweezers I have no absolute error during my prac. Thanks.

[@metball11]

this helped for my homework! thank you!!!!

[@dipanshiagarwal5205]

thank you, ma'am! I have my practical tomorrow and i missed the class where my teacher showed how to do this.

[@rusi1617]

Thank you so much maam , this video would surely help me to score well in my practical exam tomorrow

[@safbeafbe]

Thank you! I found this really useful! I'll have no problem with my science lesson now 😃

[@TheDro]

If you cool down the onion before cutting into it (just before freezing), it should help with the stinging. So does wearing goggles and cutting the onion under a kitchen vent while it's turned on.

[@tanveersingh9158]

Thank you!!! Nice humour in between

[@TheNekan]

Hey thanks for this.... looking forward to more videos from you. Watching from Colchester in England

[@bobbarcker7329]

Thank you for this video!

[@danielleczarina5103]

Thank you so much professor ;) Ur kids are adorable :D

[@keelezeibel3521]

great video and this will surely help my experiment tomorrow!

[@magedal-sheshtawi340]

Thanks very much you are very helpful thanks

[@sol_music]

This helped me in my science test
Thank You SOOOOOOOOOOO Much
regards from INDIA

[@jamesblanco7878]

I saw that too in microscope!
We use that onion skin.

[@ScienceProfOnline]

Fantastic! So glad it helped :)

[@AlexanderShubin555]

Hello! Thanks a lot for you video! I've subscribed to you and go to your interesting site! I have 3 kids!

[@gurleenkaur5331]

hey i want to tell that the way you put coverslip is wrong...if u do this way there is a risk of air bubbles to come in.....so its better to touch the coverslip with iodine on one side and second side not touching and then keep the tweezers and the slowly put it down from the other side well i liked it the way u take off the epidermal layer in the second way.

[@jackman5262]

im expecting a microscope to come in and im looking up experiments. im definitely going to try this. thx

[@PIXELinCUBE]

hey, this year we started do labaratory stuff, there was a test whit mocroscope to see onion cell, but all i see was somethink like crooked line, what i did wrong ?
( Sorry for english)

[@saina1331]

I have a practical test on this tomorrow, and I've never done a wet mount before, any tips on how to make sure I don't get any air bubbles on my slide?

[@powerplayer957]

make more video like this

[@abdssamadkhashan8556]

merci a tou les information

[@ScienceProfOnline]

+Abdssamad Khashan
de rien :)

[@eaglewolfzen]

Has anyone noticed what this says about epigenetics? There are no chloroplasts, those genes are not expressed.

[@ScienceProfOnline]

+Pimpmastahanhduece
Interested in epigenetics? Have you heard this episode of Radiolab? Inheritance: www.radiolab.org/story/251876-inheritance. I use it in teaching my college biology courses. Very thought-provoking.

[@elonan.5495]

Thanks a lot :)

[@cutenessoverload96432]

Yo hize eso en mi clase de biologia

[@omairatalon4932]

wow!love these...:-)

[@sonikaprakash9381]

yes it ll work..

[@rajanikumari8733]

very useful

[@rosariojacoby5260]

Hey, with which microscope did you do it? Because I'm want to buy one but don't know which. Thanks!!