Bioinformatics - Understanding Trimmomatic

[@seulalee2024]

Thank you so much for the help. I was struggling with understanding the parameters and now I understand the basic concept.

[@CooktoCope]

Thank you so much for the lesson.. However, I didn't quite get the concept of paired and unpaired output files. Can you please elaborate?

[@manuelargos]

Gracias. Este video es muy bueno. Que bueno eres explicando el proceso

[@alvaroruiztabas5627]

I really appreciate this videos, thank you very much Alex. One question, how do you know the illuminaclip that you nedd when you download or receive fastq data? Thanks again

[@anindogamingbd2512]

Hlew sir if i want to trimm data before allignment.. For example if i take 2000 covid data from server and want to align it. Then what should i do first to trimm those 2000 data and then i can run the allignment and use it for future works. Or can i align data without trimming will it be significant?
Thanks in advance sir

[@citron2725]

very nice work done Alex, hope to see more videos:)

[@Caroline-vy1co]

Thank you Alex

[@marwatawfik3956]

Do you recommend using trimmomatic for quality control check of microbiota (16s rRNA)?

[@alexsoupir]

That would depend on the methods used for sequencing but usually it you send got just 16S you wouldn't need to.

[@marwatawfik3956]

@@alexsoupirThanks Alex, I found you have experience with microbiota from your GitHub, so, I was asking because most people that got 16s data from our university use trim Galore, while I used trimmomatic before for RNAseq so thought why use a new tool for trimming.
Cheers
Marwa

[@soura]

just like the phred score my understanding decreased over time :)