An Introduction to Scaffolding with Hi-C Data in Galaxy

Advances in sequencing technologies have revolutionized the field of genomics, allowing for a reduction in both the time and resources required to assemble genomes de novo. One of the challenges in de novo genome assembly is the reconstruction of haplotypes, which is an important resource in analyzing variants and heterozygosity. For downstream utility, scaffolding is needed to decipher the order and location of contigs along chromosomal boundaries.
In this hands-on bioinformatics webinar, learn how you use the Galaxy-VGP workflow with Hi-C and long read data to scaffold your genome to achieve more accurate and contiguous genome assemblies.
What You Will Learn
1. What Hi-C data brings to de novo genome assembly. Understand the fundamentals of genome assembly and see how Hi-C data helps generate high-quality, phased reference genomes.
2. How you can assemble your genome for free with state-of-the-art workflows on Galaxy using long read and Hi-C technology. Learn how to access genome assembly workflows created by the Galaxy Project and the Vertebrate Genome Project (VGP), and how to run them easily on a public Galaxy instance.
3. How Hi-C data is used for assembly curation. Explore how visualization of Hi-C data provides precious information for chromosome identification and manual reorganization.