Ultramicrotomy: The Art of Sectioning for TEM
HTML-код
- Опубликовано: 25 авг 2024
- 1. Trimming
2. Small Section
3. Large Sections
While learning how to section I found it difficult to understand the concepts without a visual aid. For my final project in the TEM course I created this video to aid other visual learners. I apologize for the poor quality but hope that this video is informative.
This video is for educational purposes only.
Things are much simpler these days. There are many unnecessary steps and techniques included in this video that are not currently needed, and the ultramicrotome has advanced drastically.
Great video! Thanks for uploading. Music made it even more epic ;)
Really good explanation of the steps involved! Thank you!
This will be great for my students! It's so hard to crowd around a microtome, but this video will help with that! :)
Where do you teach?
This was so satisfying to watch
Excellent video. Thank you for this!
i am appreciating the tron soundtrack
This was so helpful thank you very much❤️
Very useful,,,Thank you very much!
Thank you.
thanks a lot, this video is very useful
Yes very good! Thank you!
Very useful,thx!
Grate sir
wow, so cool, ty
How small is 70nm just curious
Smaller than the diameter of the coronavirus. Coronavirus is approx. 100-120nm
what camera did you use to record this??
Just the camera on my phone
@@danielvocelle1414 did you just mount the phone?
@@junglebaby448 yes, built a holder out of wood to hold it on the objective
i dont think that it is nessesary to make marks for trimming becuase anyway you are not cutting on the mark
I hate the soundtrack. Makes it sound like something horrible is going to happen.
Thanks Mary
Block too far out of the holder.
Dreadful score marks on the sections - throw that knife away ! Fit a new one or use a diamond knife
Happy to report knife was thrown away 10 years ago.
I'm glad you did! I started doing ultramicrotome in 1966 using a completely manual Cambridge ultramicrotome, with glass knives, cutting phloem tissue. Very difficult!
I moved to the Institute of Ophthalmology in 1971 and finally ended up using a Leica ! I retired in 2011,after seeing many changes in TEM and SEM
Happy cutting !