How to isolate RNA from tissue or cells

Learn how to isolate ultrapure total RNA within an hour, even from difficult samples with the TRIzol™ Plus RNA Purification Kit. The isolated total RNA may be used for RNA sequencing, RT-PCR, and microarrays. The TRIzol™ Plus RNA Purification Kit is based on TRIzol Reagent lysis technology and PureLink™ silica-membrane purification technology. This combination provides highly pure RNA even from fibrous tissue.
The complete protocol can be viewed here: tools.thermofi...

[@91Ferhat]

It says 0.4uL's ~1:35 but I'm pretty sure 400uL is pipetted. They probably meant 0.4ml.

[@Squire19lUK]

So glad you posted this, thought I heard that wrong

[@muharremsahin2522]

I was confused. Thanks

[@normanchan3842]

Same here, thanks

[@jconti123]

Discod the flow through

[@lbb4974]

Not working in the fume hood when handling chloroform? o.O?

[@MrFilu13]

Good explanation

[@jimmylao349]

It sounds a very formal training, Thanks!

[@farshidardabili5101]

In rna extraction with trizol why the upper phase is red, phenol-chloroform phase and the lower phase is aqueous ?

[@mkansari481]

greetings! can this kit be used for RNA extraction from blood samples (Human)??

[@noor14-5]

Recovery tube what is it used for?

[@jesuscano6697]

Where's the test kit where ai verify the sequence of the vaccine?

[@emilybelt1564]

No one knows it's a company secret

[@shamitaumakandan3566]

Is ethanol not required to precipitate and wash RNA?

[@EugeniasmirnaGonzalezDiaz]

Excellent

[@madeehaafzal9282]

AT WHICH POINT WE HAVE TO ADD LYSIS BUFFER THAT COMES WITH KIT?

[@Menanonyung]

I think they used Trizol as a lysis buffer instead of lysis buff from the kit

[@shandhyadebnath6527]

Could you please tell me the centrifuge temperature in case of GeneJET RNA purification?
Thanks.

[@thermofisher]

Thank you for your question Shandhya. The centrifugation steps in the GeneJet RNA purification kit can be performed at room temperature.
For any additional technical questions, please contact us at thermofisher.com/askaquestion. Thank you!

[@maihien9245]

Some segments in the video are stamped not adjacent to each other

[@thermofisher]

Thank you for watching the video. We'll share your feedback with the team.

[@greenspectrum334]

do we have a research article we could read that used the exact procedures?

[@nf3922]

5 hours later... and that's all there is to it. cDNA.

[@Roberco]

Anyone have success with this using adipose tissue?

[@thermofisher]

Thanks for the question. Yes, the Trizol Plus kit can be used with adipose tissue. However it is important to use a higher amount of tissue (50 - 100 mg) if possible so that you get enough cells. We also recommend a strong homogenizer to really break up the tissue.