One-step TUNEL (Paraffin Section) Operation Guide

This video is about how to operate Elabscience® One-step TUNEL (Paraffin Section) experiment. For each reaction, there is a demonstration, so that the experimenters can better understand the TUNEL process.
Procedure:
Paraffin Section → Dewaxing → Washing → Permeabilization → Washing → Equilibrium → Labeling → Washing → Nuclear staining → Washing → Mounting → Analyze sample
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[@stewiegriffin5275]

Thank you!!

[@husnainahmad4749]

very clear explanation. i have pollen samples, I followed this protocol. But, my pollen samples don't get attached to the slide surface. What could be the reason and solution?

[@Elabscience_Bio]

We have not verified pollen samples, but the use of anti-stripping slides can reduce the incidence of stripping.

[@nabilajwad390]

Can I use 3D bio-printed to study the apoptosis assay using this kit? Or do the 3D models need sectioning first? Thank you!

[@Elabscience_Bio]

The models need to be sectioned. This assay includes a permeabilization step, and if the sample is too thick, it may not be fully permeabilized. Typically, paraffin sections are 4-6 μm, and frozen sections are 5-10 μm.