sir please refer link of guideline EDQM Guideline www.edqm.eu/documents/52006/128968/omcl-quality-management-document-annex-1-qualification-of-hplc-equipment.pdf/026e275c-2867-c216-9576-1b62ac13ef4e?t=1628491788491
First of All thanks for such a brilliant question. If you see guideline General European OMCL Network (GEON) as per that we need to scan from 190 to 290 nm scan with 1 nm incremental. And if you scan from 190 to 290 with 1 nm incremental your analysis time will huge. That’s why HPLC vendors recommends to scan near 202 to 208nm. Rather than entire 190 to 290nm. Hope it’s clear to you now..
Very useful for pharma quality guys... Good job
thanks Rajesh
Super sir
In uv detector above 300nm how to perform
Sir isme detector kon sa detector used karte h pda or uv
Neeraj mene UV detector ke hisab se kiya he explain
Thanks sir
😊thanks🙏 ur response
Welcome Sarita Sawji
In which guideline it is mentioned maxima should be ± 2nm plz suggest
sir please refer link of guideline EDQM Guideline
www.edqm.eu/documents/52006/128968/omcl-quality-management-document-annex-1-qualification-of-hplc-equipment.pdf/026e275c-2867-c216-9576-1b62ac13ef4e?t=1628491788491
Sir actually our limit is 203-207 nm.but why we are doing 202-208 nm please explain
First of All thanks for such a brilliant question.
If you see guideline General European OMCL Network (GEON) as per that we need to scan from 190 to 290 nm scan with 1 nm incremental. And if you scan from 190 to 290 with 1 nm incremental your analysis time will huge. That’s why HPLC vendors recommends to scan near 202 to 208nm. Rather than entire 190 to 290nm. Hope it’s clear to you now..