Wavelength Accuracy || HPLC Calibration Series Part-2 ||
HTML-код
- Опубликовано: 5 дек 2024
- Chapaters in this video
00:00
0:18 Introduction
00:30 Detector Calibration parameters
00:46 Wavelength accuracy test
01:07 Requirements to perform wavelength accuracy test
01:30 Caffeine standard solution 25 ppm preparation
02:09 Wavelength accuracy test instrument method parameters
03:41 Wavelength accuracy test acceptance criteria
04:52 Why Caffeine used for wavelength accuracy test
Very useful for pharma quality guys... Good job
thanks Rajesh
Super sir
In uv detector above 300nm how to perform
In which guideline it is mentioned maxima should be ± 2nm plz suggest
sir please refer link of guideline EDQM Guideline
www.edqm.eu/documents/52006/128968/omcl-quality-management-document-annex-1-qualification-of-hplc-equipment.pdf/026e275c-2867-c216-9576-1b62ac13ef4e?t=1628491788491
Sir isme detector kon sa detector used karte h pda or uv
Neeraj mene UV detector ke hisab se kiya he explain
Sir actually our limit is 203-207 nm.but why we are doing 202-208 nm please explain
First of All thanks for such a brilliant question.
If you see guideline General European OMCL Network (GEON) as per that we need to scan from 190 to 290 nm scan with 1 nm incremental. And if you scan from 190 to 290 with 1 nm incremental your analysis time will huge. That’s why HPLC vendors recommends to scan near 202 to 208nm. Rather than entire 190 to 290nm. Hope it’s clear to you now..
Thanks sir
😊thanks🙏 ur response
Welcome Sarita Sawji