How to correctly analyze raw sequence files of bacterial 16s rRNA partial gene sequence

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[@victoriaogor4525]

Very useful sir, thanks.

[@user-wv7dn6ux5p]

Thank you so much for making this fantastic video. I have watched 10 videos by after this video all doubts become clear. if it is possible, please make some video on shotgun whole genome sequencing of bacteria.

[@dr.mdashrafulislam2864]

need more video about analysis. thank you very much

[@repallyayyanna7849]

thanks, its useful video

[@endalineaniefuna9304]

Thanks a lot!

[@anjalighosh13]

Sir forward primer always binds with anti sense strand and reverse primer binds with sense strand.

[@mekpath5818]

Thank you so much

[@bjjjallowjallow7844]

how did you get the chromatogram in bioedit. i am only getting the sequence in bioedit and did not know how you opened both the bioedit and chromatogram to delete uncorrect nucleotides.

[@drmrunalinibr2869]

Extremely good explanation

[@DweipayanG]

Thank you !!

[@kristinacasandrapava9252]

Hi, what do you do when there are gaps between the sequences after aligning them?

[@blessy945]

sir the lesson is useful. can u also make a video on "how to align our trimmed consensus 16s rRNA sequence with BLAST hit sequences, including some whole genome sequences hits in MEGA software". Thank you

[@khanhammaadabdulwahab2000]

From where we can download forward and reverse sequences?

[@user-wv7dn6ux5p]

please give the website link of bioedit software, bcz there is more than 3-4.

[@noorlutphyali7495]

The quary length in this example is 403 , i used the same step and i get 665, please i want to ask is this query length acceptable to be be submitted to NCBI to register the bacteria ?

[@kajalpanchal8239]

Hello sir, does forward mean 5 prime to 3 prime?

[@DweipayanG]

Forward mean , the amplicon that u get by extension of forward primer

[@thehippocampus9130]

How about for 4peaks?

[@Zahid.Mumtaz]

Please guide me, I am stuck as my consensus sequence is coming in this form. what to do? Example
>Consensus -------------------------------------------CTGCCAGTAAGACAGGGATAACGCCCGGAAAC-GAAGCAAATAACGCATA--AACCACTCCGCCAGGGGAG-ACGATCGAAAGACGGAAACGCAAAGCAATT---GACGGGCCCCCGCA-CAAGAGCTAGAGCATGAGGTA-AAGGCGAACCAACGCAAAGAACCATACCAGACCTG-GACA-TCATCGGACA-ACT----CT-AGACCAAAAAACAAGGCTCAACCC-GGAGGGTAAGCGGAAACTCGCCAGCCCGAAA-CACCCAGATAATCC-------------------

[@rupahalder6723]

Sir i have some problems to solving Blast. Can i contact with you through mail or contact number ?