Loop-Mediated Isothermal Amplification (LAMP): Primer Design and Assay Optimization

Nucleic acid based detection assays hold great promise for improved speed and sensitivity for a variety of applications such as microorganism detection, disease diagnosis and more. Loop-mediated isothermal amplification (LAMP), an isothermal nucleic acid amplification method which is rapidly gaining popularity, provides assay developers with a fast and cost-effective alternative to PCR for nucleic acid detection. LAMP’s isothermal characteristics allow for the development of simple, robust, low cost assays that can be deployed for point-of-care, point-of-need or environmental testing. Although a developed LAMP assay provides robust detection capabilities, initial design and development can prove difficult due to the 4 (or 6 for the faster assays) primer requirement. The need for 6 primers targeting 8 regions of the genome, the distance requirements between the primers, and sequence variability among clinical isolates of a given species put significant constraints on primer design. Improper primer design or selection leads to suboptimal assays resulting in slow amplification, non-specific amplification, and poor sensitivity. This webinar will review the basic mechanics of LAMP and how it may be used as a diagnostic assay. We will look in-depth at primer design with a focus on the use of primer design software and how specific settings improve primer design success specifically with LavaLAMP™ Enzyme-based Kits. We will also discuss optimization techniques to get the most out of your assay design.
This webinar will:
Review the mechanics of LAMP
Examine the primers required and their design
Learn how adjust software setting for better primer design
Demonstrate the effects of optimizing primer design on LAMP assay results
Explore additional ways to optimize LAMP assays
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