at 60-65°C, the DNA is partially single-stranded (depending on its GC-content), which is enough for some primers to initiate the LAMP-reaction. I think the majority of the DNA remains double-stranded, but it doesn't matter. If your target sequence has a high GC-content, though, it may happen that 65°C is not enough for LAMP initiation and you would need to elevate temperature stepwise.
Hey there, do you maybe have a good source for the tipical yields of PCR and LAMP, that you give in the video? Can't finde a citable one. Thanks in advance!
Hi, i have question, why i got this "Could not generate Primer set(s). Please change parameters or target" statement when i tried to design my ITS region? I tried to do some changes in Detail Settings, but i still received that. Please help. Thanks in advance!
Hello Atiquah! We would be happy to help with your primer design questions. Please contact us at techsupport@lucigen.com as the root cause could be a number of things. Have a great day!
Thanks so much for the concise explanation! Best video on the subject I watched so far.
You explain very well! Thank you so much fo saving my thesis
Hi I am a bit confused at 60 -65 is DNA double stranded or single stranded, if it is double stranded how does FIP primer binds?
at 60-65°C, the DNA is partially single-stranded (depending on its GC-content), which is enough for some primers to initiate the LAMP-reaction. I think the majority of the DNA remains double-stranded, but it doesn't matter. If your target sequence has a high GC-content, though, it may happen that 65°C is not enough for LAMP initiation and you would need to elevate temperature stepwise.
Great video!
thank you for the informative content
could you come with Alethia LAMP? thanks
Hey there, do you maybe have a good source for the tipical yields of PCR and LAMP, that you give in the video? Can't finde a citable one. Thanks in advance!
Thanks, informative webinar
Hi, i have question, why i got this "Could not generate Primer set(s). Please change parameters or target" statement when i tried to design my ITS region? I tried to do some changes in Detail Settings, but i still received that. Please help. Thanks in advance!
Hello Atiquah! We would be happy to help with your primer design questions. Please contact us at techsupport@lucigen.com as the root cause could be a number of things. Have a great day!
good contents friend
Very informative, thank you
Many thanks
No one voted in the poll 😢
Helpful, thanks
how to design loop primer in detail explanation
It should be a complimentary sequence to that region where you expect the loop to form