Loop-Mediated Isothermal Amplification (LAMP): Primer Design and Assay Optimization

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  • Опубликовано: 23 дек 2024

Комментарии • 18

  • @testboga5991
    @testboga5991 2 года назад

    Thanks so much for the concise explanation! Best video on the subject I watched so far.

  • @katkat3458
    @katkat3458 Год назад

    You explain very well! Thank you so much fo saving my thesis

  • @shalinipeeris9244
    @shalinipeeris9244 4 года назад +1

    Hi I am a bit confused at 60 -65 is DNA double stranded or single stranded, if it is double stranded how does FIP primer binds?

    • @gutestederwelt
      @gutestederwelt 4 года назад +3

      at 60-65°C, the DNA is partially single-stranded (depending on its GC-content), which is enough for some primers to initiate the LAMP-reaction. I think the majority of the DNA remains double-stranded, but it doesn't matter. If your target sequence has a high GC-content, though, it may happen that 65°C is not enough for LAMP initiation and you would need to elevate temperature stepwise.

  • @henrikslab
    @henrikslab 3 года назад

    Great video!

  • @MandeepSingh-lk2nz
    @MandeepSingh-lk2nz 3 года назад

    thank you for the informative content

  • @mengistuhailemariam9050
    @mengistuhailemariam9050 4 года назад

    could you come with Alethia LAMP? thanks

  • @lubra9097
    @lubra9097 6 лет назад

    Hey there, do you maybe have a good source for the tipical yields of PCR and LAMP, that you give in the video? Can't finde a citable one. Thanks in advance!

  • @serhiysouchelnytskyi4697
    @serhiysouchelnytskyi4697 3 года назад

    Thanks, informative webinar

  • @ciktiqshafaz
    @ciktiqshafaz 6 лет назад

    Hi, i have question, why i got this "Could not generate Primer set(s). Please change parameters or target" statement when i tried to design my ITS region? I tried to do some changes in Detail Settings, but i still received that. Please help. Thanks in advance!

    • @LucigenVideo
      @LucigenVideo  6 лет назад

      Hello Atiquah! We would be happy to help with your primer design questions. Please contact us at techsupport@lucigen.com as the root cause could be a number of things. Have a great day!

  • @zaccomusic
    @zaccomusic 4 года назад

    good contents friend

  • @Soothyourminds
    @Soothyourminds 6 лет назад

    Very informative, thank you

  • @mengistuhailemariam9050
    @mengistuhailemariam9050 4 года назад

    Many thanks

  • @zachp2120
    @zachp2120 10 месяцев назад

    No one voted in the poll 😢

  • @hailongwang1017
    @hailongwang1017 6 лет назад

    Helpful, thanks

  • @krishnachandra3306
    @krishnachandra3306 4 года назад

    how to design loop primer in detail explanation

    • @henrikslab
      @henrikslab 3 года назад

      It should be a complimentary sequence to that region where you expect the loop to form