Best EMSA video ever! You just pulled together things that I have been seeing in peer-review articles (like supershift) and didn't know how to explain. THANKS!!!
Hello! Very nice video, thank you so much! Do you think that after emsa could we take out the bands (free DNA vs DNA+protein complex band), purify them (carefully bcs we have redioactive P) and sequence them in order to see at which exactly DNA seq the proteins are bounding? You think is feasible?
This video was really amazing. I am interested in EMSA, and I want to know can supershift could quantify RNA-protein interactions? such as a quantitative measurement to know if additional protein that binds to the first protein could reduce or increase the interactions? Thank you very much...
For example, we have two scenarios 1) we have two protein (A and B) A would bind to DNA and B would bind to A protein and 2) both A and B protein bind to DNA. Can we differentiate between these two conditions? because on both these scenarios we will have supershifts, correct?
Best EMSA video even in 2024
Best EMSA video ever! You just pulled together things that I have been seeing in peer-review articles (like supershift) and didn't know how to explain. THANKS!!!
Seconded.
Love the step by step explanation of the results and why certain bands appear and others dont. Thank you
This is clear and concise. I have just realized that I don't analyze my gels so well. There's so much a gel can tell you from the bands.
The best explanatory video ever!!!
I will never be depressed if the professor starts explaining stuff like this
The best understanding of EMSA TKS!!
Awesome and concise explanation!
Thanks. I find all the relevant content in your videos.
WOW, great video! thanks a lot
perfect explaining thanks !
God Bless you!!
Great job! Thanks!
your english is amazing
Wonderful explanation
This is a fantastic video and I thank you a lot
Thank you🫂
Thankzzz maam.... that was really awesome...
Thank u so.much
nice video. good explanation
Hello! Very nice video, thank you so much! Do you think that after emsa could we take out the bands (free DNA vs DNA+protein complex band), purify them (carefully bcs we have redioactive P) and sequence them in order to see at which exactly DNA seq the proteins are bounding? You think is feasible?
Woho...awesome. Thanks for that nix explanation! :)
Amazing!
This video was really amazing. I am interested in EMSA, and I want to know can supershift could quantify RNA-protein interactions? such as a quantitative measurement to know if additional protein that binds to the first protein could reduce or increase the interactions? Thank you very much...
thanks for the great video and clear explanation
cool vid :3
Super Clear! Thank you!
For example, we have two scenarios 1) we have two protein (A and B) A would bind to DNA and B would bind to A protein and 2) both A and B protein bind to DNA. Can we differentiate between these two conditions? because on both these scenarios we will have supershifts, correct?
Deletion/ Mutational analysis of the DNA sequence.
amazing, thank you so much!
Such a great explanation. Thank you so much!
Thank you!
THANKS!!!!
YOU ARE A GD SEND!!!! THANK YOU!!
So good love it
comprehensive
This is money
Plp
Thank you!