Passaging is just the moving of cell from one media to fresher or another medi, while cell line is the cell from one primary culture passaging to the new culture to cntinue the cell line :) , right?
When we do trypsinization, growth factor receptors are also digested as they are membrane bound proteins. Then how does the primary culture cells respond to the artificial growth factor we provide in the media?
Wait isn't passaging using the same idea as subculture in which the cells within the organism are placed into another fresh media in order to create a Primary Cell Line? Cause I am now completely confused on the concept....
I must say you are doing an excellent job, clearing all the confusions. keep it up.
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My favorite educational RUclips Channel. God Bless you as you keep helping us
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Thank you! Truly recommend all your videos.
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Excellent teaching way😊
Thank you
Thank you Sir,for this explanation.
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Thank you very much
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Well explained😊
Thank you
Suman, do you have a video on the syn and anti conformations of DNA nucleotides? and what about puckered confirmations like endo and exo? Thank you!!
Question to think about: In vivo , when the cell divide , how many divisions it takes to reach death? is it the same time as individual dies?
thank you dear
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Passaging is just the moving of cell from one media to fresher or another medi, while cell line is the cell from one primary culture passaging to the new culture to cntinue the cell line :) , right?
Great!
Thank you
Super
Thanks
Sir make a video on hela cell culture and their characteristics
Okay
When we do trypsinization, growth factor receptors are also digested as they are membrane bound proteins. Then how does the primary culture cells respond to the artificial growth factor we provide in the media?
Trypsinization does not damage the membrane. It just breaks down the Cell Adhesion Molecules. Cell adhesion is promoted by CAMs and ECM.
@@ashishsengupta6980 Thank you :)
Q: Do the suspension cells do not have contact inhibition upon confluence?
But...in previous lecture you said passing cells from primary culture to next sec. culture is called subculturing... are both the same ?
Growth phases are four type so what is the stage of subculture can u explain about that sir
Every subculture will go through all 4 phases of growth
Great video. You look so cute on 7:08 min :)
@SwiffVideo what's bad ?
oh you're "thirsty" for knowledge alright
Down bad
Passage is only the transfer process.
Plzz tell any biotechnology channel ....no one teaches it.....there are a bunch of channels on biology ...but not on biotechnology .....plzz help
This channels fulfills 90% of biotechnology topics
@@shomusbiologyofficial thanku sir 👍👍
why petri dish are not to be use ?
How can we know of cell's passage # ?
Can we find the age of a person by doing passeging of cells
No
Wait isn't passaging using the same idea as subculture in which the cells within the organism are placed into another fresh media in order to create a Primary Cell Line? Cause I am now completely confused on the concept....
Nevermind forget I said this.....
how to transfer animal cell
Why trypsinisation
👍
What book you use for the references?
You can use Stanbury and Whittaker (fermentation
why hella cells show that properties of culture
Sir ia ma watching after 6 year
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Hindi main samjaya kro
sexy
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