Stage 2 of 3: Generation of Stable, Transfected Cell lines: Transfection

This second video of our three-part series about generation of stable, transfected cell lines demonstrates transfection of an antibiotic-sensitive parental cell line with a plasmid containing a resistance gene to a selection antibiotic after performing a kill curve experiment. In this video, we use the selection antibiotic, G418 Disulfate (Geneticin) in combination with CHO cells. This is the second step of generating stable, transfected cell lines.
A link to the protocol can be found here: www.toku-e.com/Assets/Protocol...
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[@KiranKumar-gs7mp]

Very nice demonstration.....