queen, you are the overflowing wellspring of everything biochemistry. I'm pretty sure if anyone watches all your videos they should have an honorary Masters in Biology😂
Oh man, I remember doing this, lots of troubleshooting in every step. Good times. And the need to keep track of the manufacturers! It's crazy how sometimes the manufacturer of the same kind of resin can totally change the purification results!
Can you show me how to determine if the protein you want to purify undergoes native or denaturing conditions (protein solubility)? And why the His-tag residues amount must be 6 or 8 but not 7?
I think this will help with your first question bit.ly/wherestheprotein ruclips.net/video/jTQxHK3o3lE/видео.html not sure about the # of His - guessing just convenience
queen, you are the overflowing wellspring of everything biochemistry. I'm pretty sure if anyone watches all your videos they should have an honorary Masters in Biology😂
Oh man, I remember doing this, lots of troubleshooting in every step. Good times. And the need to keep track of the manufacturers! It's crazy how sometimes the manufacturer of the same kind of resin can totally change the purification results!
Can you show me how to determine if the protein you want to purify undergoes native or denaturing conditions (protein solubility)? And why the His-tag residues amount must be 6 or 8 but not 7?
I think this will help with your first question bit.ly/wherestheprotein ruclips.net/video/jTQxHK3o3lE/видео.html not sure about the # of His - guessing just convenience
@@thebumblingbiochemist Thank you very much!