WEBINAR: Cell-based and Biochemical High-throughput Screening in 1536-well Plates

In this webinar, speakers Dr. Gene Ananiev, Dr. Carl Peters and Dr. Iain Russell discussed two projects. In one of the projects, they demonstrated the creation of a robust FMR1-Nluc reporter hiPSC line by knocking in a Nano luciferase (Nluc) gene into the endogenous human FMR1 gene using the CRISPR/Cas9 genome editing method. We confirmed that luciferase activities faithfully report FMR1 gene expression levels and showed that neural progenitor cells derived from this line could be optimized for high-throughput screening.