To Prepare Stained Temporary Mount of Onion Peel | Science | Class 9 | Term 1 Practical |CBSE |NCERT

Aim : To Prepare Stained Temporary Mount of Onion Peel
Procedure
Pour some distilled water into a watch glass.
Peel off a leaf from half a piece of onion and using the forceps, pull out a piece of transparent onion peel (epidermis) from the leaf.
Put the epidermis in the watch glass containing distilled water.
Take a few drops of safranin solution in a dropper and transfer this into another watch glass.
Using a brush, transfer the peel into the watch glass containing the safranin solution.
Let this remain in the Safranin solution for 30 seconds, so that the peel is stained.
Take the peel from the Safranin solution using the brush and place it in the watch glass containing the distilled water.
Take a few drops of glycerine in a dropper and pour 2-3 drops at the center of a dry glass slide.
Using the brush, place the peel onto the slide containing glycerine.
Take a cover slip and place it gently on the peel with the aid of a needle.
Remove the extra glycerine using a piece of blotting paper.
Place this glass side on the stage of the compound microscope and view it.
Observations
There are a large number of regularly shaped cells lying side by side and each cell has a distinct cell wall.
A distinct nucleus is present on the periphery of each cell.
Lightly stained cytoplasm is observed in each cell.
A large vacuole is present at the centre of each cell, and is surrounded by the cytoplasm.
Conclusion
As cell walls and large vacuoles are clearly observed in all the cells, the cells placed for observation are plant cells.
Precautions
Use a brush to transfer the peel from one apparatus to another.
Staining of peel should neither be too dark, nor too light.
Extra glycerine stain should be removed using blotting paper.

[@PWLIVE810]

Legends are here because there exam is tomorrow

[@Atuleshpandey1605]

So damn correct

[@Atuleshpandey1605]

💯💪💪💪

[@syeedsameen7459]

Exactly,👌

[@rachitnishad2727]

Yup

[@Aditya-ku6lb]

ABE HAR JAGAH KYU AAJA HAI BE BSDK

[@IshaRt1999]

Tomorrow is my practical thanku man

[@AbhinavKumar-jv5od]

I think you should use glycerine before transferring the peel to the watch glass add 2-3 drops of glycerine into the watch glass

[@user-rx8ql2ss5j]

Thanks a lot mam i have a bio practical on monday and this video helped me a lot once again thanks a lot mam

[@PRITAMSINGH-zi9xv]

Thankyou ma'am ❤️👍

[@jairajv6307]

How to use microscope...and how to place slides correctly.. ...please do a video on this

[@adhartjohny4865]

Thank you mam

[@supereasy9997]

All the best for tom practical

[@pari8211]

Great👍👍👍👍

[@panwargamerz7592]

Thankyou mam tommrow is my practice

[@pritimehrashorts]

Thanks for this video sis

[@kaifbanglewale5167]

How to use microscope and how to place slides correctly please do a video on thise

[@narendraparmar-R]

👌👌

[@LUCIFER-ly3uz]

THANKYOU MAM FOR THESE EXPERIMENT 🙏🥰

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Jazakallah 😊

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Ty mam 😘♥️

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Very nice active❤❤❤

[@varunnekarnvarun1375]

Thank you❤🌹🙏

[@anudhiman4734]

Haaha today is my practical and viva and im watching this video in the morning😅😅

[@andhalescreationsofd.i.y4399]

😅😅😅 exam tomorrow 😁😁😁
💜💜💜 Whole year gone
Watching BTS MV on
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[@nilamverma987]

Thanku mam

[@SiddharthRaju-dr2eh]

is this in okaridge international school?

[@NeelamNeelam-e7y]

Thak you mam

[@mandalhacker2582]

What type of microscope this is. I want to know the price range

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❤️❤️

[@mhamadullamondal8882]

Mam we take Petri dish also

[@ajitgopalkrishnan3348]

Baat to sahi hai

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Le legends ,passing marks to aa hi jayenge.

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Ese pariyog ke liye aapna bahut khon nikal diya par kar nhi pate the mam

[@hr10gameing8]

Bro my practical is tomorrow 😅 😂

[@VinkathaShivareddy]

Mam what is stain name pls say 😊😊❤

[@ChemXpert]

Safranin

[@PBXSingh]

Saint Paul ala hega koi

[@pihuaarush2001]

Humare yha Jo microscope hai usme saf nhi dikhta Kya Kare

[@3813AmanSharma]

first time I have seen in microscope

[@pari8211]

Yeah same here

[@kummariramulamma9644]

Same

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Analysis

[@vanshika1328]

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tomorrow is exam

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[@harshilvishnukumarchavada6005]

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Hi

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Thanks mam