Hi! I have a problem: When I click on Three-Way ANOVA, the following message appears: "You have not entered the right king of data for this analysis" What could I do in order to solve this issue? Thanks a lot.
Thank you for the video. I have a data set I wish to analyse via the 3-way ANOVA in Prism. As per the example, I have 1 measurement variable and 3 nominal groups (dietary condition (3-levels), visit (2-levels) and measurement point (2-levels). Unfortunately, it seems that the 3-way ANOVA in Prism does not allow for this number of levels. In other words, it allows you to enter data with 4 but not 6 groups, as would be required for my analysis. I wonder if you have encountered this issue or could suggest a workaround? Many thanks
hi, i need to make a 3 way anova, and everything its ok until the option tab: its disabled so i cant make tukey test. do you know what may be happening? thanks a lot
hi, can you answer me please : if i want to use 3 way anova but with repeated measures (same group at different conditions), i do the same thing as the video ?
Hi, thanks for the video, super clear as for the Two-way Anova. I'm increasing my chances to get an answer by posting my question here too... : When it comes to anova, being two- or three-way, i don't really know how to biologically (or even statistically) interpret the "interaction" result. How would you explain it please ? To give a proper exemple, I'm working on two genes, and thus have 4 groupe (WT-WT ; WT-mut ; mut-WT ; mut-mut). In term of result, the simple mutated have an intermediate phenotype, and the double mutated a stronger phenotype. When I run the anova, I got something like "mutation1 : * * * * ; mutation 2 : * * * * ; mut1 x mut2 : ns". How to interpret that ? does it mean that the stronger phenotype in the double-mutated groupe is actually just the sum of the effect of mutation1 and mutation2, and that there is no "synergistic" effect ? To try to understand better this, i tried different set of data. If for the double mutated, i plot value equivalent to the simple mutated (thus, intermediate phenotype), THEN, i have something like "mutation1 : * * * ; mutation 2 : * * ; mut1 x mut2 : * ". The fact that mut1 and mut2 have both an effect on their own, but that they do not additionnante (as it actually does in my case) could be an indication of an epistasy between those 2 genes. Is it what the "interaction mut1 x mut2" is actually showing me ? Thanks a lot to you, who read it to the end, and thanks even more to you, who will answer me :) Pleaaaase
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Hi!
I have a problem: When I click on Three-Way ANOVA, the following message appears: "You have not entered the right king of data for this analysis"
What could I do in order to solve this issue?
Thanks a lot.
I have the sam problem, have you figure it out already? How to solve this problem?
Thank you for the video. I have a data set I wish to analyse via the 3-way ANOVA in Prism. As per the example, I have 1 measurement variable and 3 nominal groups (dietary condition (3-levels), visit (2-levels) and measurement point (2-levels). Unfortunately, it seems that the 3-way ANOVA in Prism does not allow for this number of levels. In other words, it allows you to enter data with 4 but not 6 groups, as would be required for my analysis. I wonder if you have encountered this issue or could suggest a workaround? Many thanks
I'm having the same problem, have you work on it? From my reading, it is impossible.
@@MrShazmiey Taken from the Prism website: "Three-way ANOVA (limited to two levels in two of the factors, and any number of levels in the third)".
You have to use the rows for your 3 levels
hi, i need to make a 3 way anova, and everything its ok until the option tab: its disabled so i cant make tukey test. do you know what may be happening? thanks a lot
hi, can you answer me please : if i want to use 3 way anova but with repeated measures (same group at different conditions), i do the same thing as the video ?
I have been struggling so much to find an answer to this, do you know it by now? 🙏
What test need to choose when compare 2 group in subcolumn
do you mean a 2-way ANOVA?
Hi, thanks for the video, super clear as for the Two-way Anova. I'm increasing my chances to get an answer by posting my question here too... :
When it comes to anova, being two- or three-way, i don't really know how to biologically (or even statistically) interpret the "interaction" result. How would you explain it please ?
To give a proper exemple, I'm working on two genes, and thus have 4 groupe (WT-WT ; WT-mut ; mut-WT ; mut-mut). In term of result, the simple mutated have an intermediate phenotype, and the double mutated a stronger phenotype. When I run the anova, I got something like "mutation1 : * * * * ; mutation 2 : * * * * ; mut1 x mut2 : ns". How to interpret that ? does it mean that the stronger phenotype in the double-mutated groupe is actually just the sum of the effect of mutation1 and mutation2, and that there is no "synergistic" effect ?
To try to understand better this, i tried different set of data. If for the double mutated, i plot value equivalent to the simple mutated (thus, intermediate phenotype), THEN, i have something like "mutation1 : * * * ; mutation 2 : * * ; mut1 x mut2 : * ". The fact that mut1 and mut2 have both an effect on their own, but that they do not additionnante (as it actually does in my case) could be an indication of an epistasy between those 2 genes. Is it what the "interaction mut1 x mut2" is actually showing me ?
Thanks a lot to you, who read it to the end, and thanks even more to you, who will answer me :) Pleaaaase