The Science Of Chromatography
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- Опубликовано: 10 мар 2024
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Today we go over the science of chromatography. This is a simple introduction to the concepts. Please enjoy :)
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10/10 would chromatography again
Did you mean 10 : 10?
nice one lol@@thepostapocalyptictrio4762
0:40. ah yes, LSD cut with.. meth
LMAO. Glad you noticed it
@@chemdelic my favorite lysergamide next to my least favorite substituted phenethylamine how could i not
@@alternatively_cameron Given the typical dosage sizes, meth cut with LSD sounds infinitely scarier than LSD cut with meth.
So essentially mescaline?
@@profpuffofficial2 no lmfao not all phenethylamines are amphetamines. lsd/meth is more in line with the DOx series
Someone should convince hyperspacepirate to build a hplc
100%; mans playing IRL Minecraft
In my first year of PhD, I was required to choose a speciality (inorganic, organic, biochem, physical, etc.) I do love synthetic chemistry so it’s between inorganic and organic. Considering 75% of your research effort of organic chemistry involves column chromatography (the other 25% involves interpreting your NMR/IR/whatever characterization techniques with 300 overlapping peaks), I decided to go with inorganic chemistry. Soon, I realized that I still have to do chromatography to make certain types of ligands. But hey, I only need to spend 25% of the time instead of 75%!
You know it's UHPLC when you see 1100 bar pressure at a low flowrate. Love it ❤
3rd Year Undergrad and this is immensely helpful! Thank you :)
Good! I actually have a instrumental analysis final so I used this as a way to study lol
🙏
Will watch this video later today. I actually bought a HPLC for my house. I am trying to get to the point where I can do extremely low cost psychedelic mushroom/pot testing for people (like $20/sample). I am getting close, but my HPLC was built in 1997 with software designed for Windows 3.1, so I am completely rewriting large chunks of stuff and trying to reverse engineer other parts. For example. the system, being designed for a FAT8 storage, will not let me run a method that will save more than 8MB of data as that's the biggest file that could have been made at the time.
Jesus christ bro, I thought the hplc which only ran on XP was bad.
The good news about those old ass machines is usually the protocols are really simple, so you can sniff and reverse engineer them pretty easily.
@@defenestrated23 yeah, I am hoping to replace everything with a LabVIEW program I'm writing. The comms are using GPIB. I havnt actually taken a look at the data yet though as I'm will writing a chromatogram viewer as the current one is terrible. Want to compare 2 runs from different times? Too bad lol. Also, the only way to get numbers out of the system is by printing as area/concentration isn't displayed on the screen at all.
ur a professional edger the way you released this a week after my orgo exam :3
Good stuff! Mass spectrometry video in the future?
Definitely!
That's a great video on chromatography
Yo, much appreciated. Luckily I was looking for a video on this exact topic (chemdelic are you spying on me 😳)
I spy on all my viewers and subs :)
@@chemdelic I figured you'd just had enough tryptamines to tap into the collective consciousness and now know what everybody's thinking at any given moment...
best chromatography video in youtube
A very clear overview of different types of chromatography. Well done!
Great video! Learnt bunches
I like the match cut highlight intro!
Nicely explained
Imagine Heisenbud using this knowledge for cannabinoids and terps analysis 😮
Ugh, still easier than separating individual cannabinoids.
Wow. That’s quite the admixture you have there at 0:40. Many things would happen should one consume “A” and “B”, but sleep would not be one of them.
Great video!
6mins in and 10/10, great explanation of tlc
The funny thing about the cost of instrumentation is how insane the new stuff is. I got a quote for $49K for a single part one time.
🐶: used hplc
🦮: new hplc
🐺: gc-ms
🦁: uplc-ms
😈: nmr
Loled at the choices of compounds in the intro
This is the best video on chromatography I've ever seen, which is kinda sad honestly.
You can do more theoretical stuff. Loved it
I was not expecting this specificity and that isn't even close to the true rabbit hole that is this form of chemical analysis. The details of it are as intricate as grabbing a handful of sand and sorting all the particle sizes by eye and sieve, then feel, then precipitate, filtering all the way to electron microscopes and sorting them on a computer screen. Nearly impossible at scale without such a technique as elution which is so so much faster and cheaper with a little bit of sample and a little bit of trusted charged particles. Awesome video. The precision required to get good results I'd reckon is a tough endeavor which is why you mention trial and error so much. Do the same thing hundreds of times and find the trend.
good vid bro..
I watched the entire video and have no idea what you just said.
Informative. Thanks for the video. I use a quasar as the ionization source in the intergalactic mass-spectrometer that is connected to my gas chromatograph. Unfortunately, the ionization chamber is the size of a galaxy, so it takes millions of years to get a reading.
BallzToDaWallz here, I’d love to see a video on Arylcyclohexylamines!!!!
Who wouldn't love (to see a video on) arylcyclohexylamines?
Interesting
Intro was a neuron activation moment 👍👍
Did you get your fume hood?
Your jedi teacher 😊😊😊❤😊😊😊
Been wondering how this worked for a while
"Speck trauma tree" is how I pronounce spectrometry :)
running hplc in a natural products lab and watching this is kinda a vibe ngl (also i approve of dissing business majors)
Cations are the cutest of ions and have a pawsitive charge 😺➕
furtastic comment
I think I smoked a bit too much, I thought this was going to be about light and colours and stuff..... But this was cool too :D
Discussion of consumables and cleaning/maintenance for each type?
Why is electrophoresis not considered chromatography?
I loved it when he said chromatography and chromatog'd all over the place.
Do this again but make it 1 hour with NMR
Allright just tell us whats the best eluent and static phase for separating lysergicamides, and also also which fractions should be of interest , lol
Uhh.. it’s in the patent…🤪
14:00 I thought it was pronounced līgand, with a stressed "I".
what does polarity mean?
Basically a separation of electric charge. This leads to a bond or chemical group having a electric dipole with one end of being partially negative and one end partially positive :)
Compound A Ayeeee
I’d take Compound B =)
Hmm this doesn’t look like something that would put me in a watch list as usual
We often ran a mixture through an undergrad - good training...and they pay tuition for the privilege.
Me watching this vid after 4 hrs of flash column chromatography
“I shit you not”
If you are looking for a chromatography method that can be used to separate bigger amounts of material and is not very hard or require special equipment (ie. you can do it quite easily) you should look at this video about DCVC (Dry Column Vacuum Chromatography (DCVC) Tutorial)
ruclips.net/video/lBNhu4kJ4Mc/видео.html
"Since then he developed some kind of speech impediment..."
WHERE'S DPH I WANNA MEET THE HAT MAN🤠🤠🤠🤠🤠🤠🤠
🎉🎉🎉🎉🎉🎉🎉🎉🎉🎉
sry but not letting the tlc plate run the full distance is just straight up bullshit.
Component mixtures like diastereomers with really close Rf's can be more easily spottet since the distance between the two spots becomes a bit larger. You can calculate the Rf value as well without any issues. The only problem is that developing a tlc plate takes a minute longer.
Zwitterion? New word!
ah yes, bisexual chemistry
so bio cem is salty
Q: Why did the white bear dissolve in water?
A: Because it was polar.
He was albino 😭🙏
0:53 its ALUMINIUM, not ALUMINUM
RAHHH🇺🇸🦅🦅🦅
🤦♂️@@chemdelic
Lol! I'm firmly on both sides of this one!!! 🤣🤣🤣
Give it up, different dialects exist
mmm
2ND
bahahahaa boi yapped about reverse phase hplc but left out the column types and the big dreaded normal phase
I would discuss that if I did a whole video on it. The video would have been really long if I did that for all of them lol
@@chemdelic lolololll ikkkk it’s a whole freaking mess but still a great video and breakdown (shared with my whole lab group)
This was a fun part of labs, but not much time was spent in lab on it
Don't worry too much about it, if you're doing organic chem you'll get to do more than enough to never want to look at a column again...
Repeat after me: Spec-tom-etry. I know you're a New York boy but you can do it sparky. LOL
Don’t correct people if you’re wrong…. spek
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